Q83GP4 (SYE_TROWT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Synonyms:	gltS
Ordered Locus Names:	TWT_209

Organism

Tropheryma whipplei (strain Twist) (Whipple's bacillus) [Complete proteome] [HAMAP]

Taxonomic identifier

203267 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119689
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00022_B
	Motif	255 – 259	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	258	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83GP4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 798470EDC1A71F42	FASTA	480	54,801
	10 20 30 40 50 60 MRDPRVRVRF CPSPTGAPHL GLVRTALFNW VFARKHGGGF IFRIEDTDAT RNREESCSQL 70 80 90 100 110 120 IDTLKWLGLD WDEGPDKGGQ FGPYYQSQRG DIYQEVLDKL LAADLAYESF STKEEIEKRN 130 140 150 160 170 180 LEAGRPIQLG YDNYDRTLSE QTKAAMREAG RTPIIRLRMD DENIAFEDLV KGEVVFTDPI 190 200 210 220 230 240 PDFALTRASG EPLYTLVNPV DDAFMKITHV LRGEDLLSST PRQIALYKAL ITIGITDYVP 250 260 270 280 290 300 FFGHLPIVMG EGNRKLSKRN PESDFYFYKA RGFIREGLLN YLSLLGWSIS NSRDTFSLSE 310 320 330 340 350 360 MIHAFDVRDV RGNPARFDYK KCLAINAYHL RELNVEDFFL RLVPFVEEML GIPLSFEQKN 370 380 390 400 410 420 SLRAICPFVQ GRVQLLTEAA EMVRFLLVDN ISVDFAVTDK EIDVLRHCLA LLNLLDTWES 430 440 450 460 470 480 AQIASCIKQA IEQFDLQPKR IFSILRLAIT GRRVSPPLFE SMQILGRSAS LNRVETFVTN

References

[1]

"Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome." Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M. Genome Res. 13:1800-1809(2003) [PubMed: 12902375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Twist.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014184 Genomic DNA. Translation: AAO44306.1.
RefSeq	NP_787337.1. NC_004572.3.
3D structure databases
ProteinModelPortal	Q83GP4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q83GP4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1482479.
GenomeReviews	Gene locus TWT_209 in contig AE014184_GR.
KEGG	twh:TWT209.
PATRIC	23996903. VBITroWhi72342_0336.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	IEWFNLD.
PhylomeDB	Q83GP4.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	TWHI203267:TW209-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_TROWT

Accession

Primary (citable) accession number: Q83GP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program