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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Tropheryma whipplei (strain Twist) (Whipple's bacillus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei258 – 2581ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTWHI203267:GJDK-224-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Synonyms:gltS
Ordered Locus Names:TWT_209
OrganismiTropheryma whipplei (strain Twist) (Whipple's bacillus)
Taxonomic identifieri203267 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma
ProteomesiUP000002200: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Glutamate--tRNA ligasePRO_0000119689Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi203267.TWT209.

Structurei

3D structure databases

ProteinModelPortaliQ83GP4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2211"HIGH" regionAdd
BLAST
Motifi255 – 2595"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
KOiK01885.
OMAiAFRCFCT.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83GP4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRDPRVRVRF CPSPTGAPHL GLVRTALFNW VFARKHGGGF IFRIEDTDAT
60 70 80 90 100
RNREESCSQL IDTLKWLGLD WDEGPDKGGQ FGPYYQSQRG DIYQEVLDKL
110 120 130 140 150
LAADLAYESF STKEEIEKRN LEAGRPIQLG YDNYDRTLSE QTKAAMREAG
160 170 180 190 200
RTPIIRLRMD DENIAFEDLV KGEVVFTDPI PDFALTRASG EPLYTLVNPV
210 220 230 240 250
DDAFMKITHV LRGEDLLSST PRQIALYKAL ITIGITDYVP FFGHLPIVMG
260 270 280 290 300
EGNRKLSKRN PESDFYFYKA RGFIREGLLN YLSLLGWSIS NSRDTFSLSE
310 320 330 340 350
MIHAFDVRDV RGNPARFDYK KCLAINAYHL RELNVEDFFL RLVPFVEEML
360 370 380 390 400
GIPLSFEQKN SLRAICPFVQ GRVQLLTEAA EMVRFLLVDN ISVDFAVTDK
410 420 430 440 450
EIDVLRHCLA LLNLLDTWES AQIASCIKQA IEQFDLQPKR IFSILRLAIT
460 470 480
GRRVSPPLFE SMQILGRSAS LNRVETFVTN
Length:480
Mass (Da):54,801
Last modified:June 1, 2003 - v1
Checksum:i798470EDC1A71F42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014184 Genomic DNA. Translation: AAO44306.1.
RefSeqiNP_787337.1. NC_004572.3.

Genome annotation databases

EnsemblBacteriaiAAO44306; AAO44306; TWT_209.
GeneIDi1482479.
KEGGitwh:TWT209.
PATRICi23996903. VBITroWhi72342_0336.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014184 Genomic DNA. Translation: AAO44306.1.
RefSeqiNP_787337.1. NC_004572.3.

3D structure databases

ProteinModelPortaliQ83GP4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203267.TWT209.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO44306; AAO44306; TWT_209.
GeneIDi1482479.
KEGGitwh:TWT209.
PATRICi23996903. VBITroWhi72342_0336.

Phylogenomic databases

eggNOGiCOG0008.
KOiK01885.
OMAiAFRCFCT.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciTWHI203267:GJDK-224-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
    Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
    Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Twist.

Entry informationi

Entry nameiSYE_TROWT
AccessioniPrimary (citable) accession number: Q83GP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: February 4, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.