Q83GN7 (RSMH_TROWT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	TWT_220

Organism

Tropheryma whipplei (strain Twist) (Whipple's bacillus) [Complete proteome] [HAMAP]

Taxonomic identifier

203267 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000108739
Regions
	Region	34 – 36	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	53	1	S-adenosyl-L-methionine By similarity
	Binding site	80	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	98	1	S-adenosyl-L-methionine By similarity
	Binding site	105	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83GN7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 57243EC6FA2FBD1B	FASTA	317	35,636
	10 20 30 40 50 60 MNGHLPVMLG EVCQLIEPVL GIGDVFIDAT LGAGGHSEAI LMSSQGALLY GIDRDAYALA 70 80 90 100 110 120 LARKRLSGFA DRCKFVHDTF DKFDKYLSNL HPKVFLFDLG MSSMQIDNPD RGFSYMKSGP 130 140 150 160 170 180 LDMRMNESDK ITAKEILNGY SETALIRIFR DYGQERYAKR IARQICKARS VSELVTTCQV 190 200 210 220 230 240 SQLIRDVCPP HIRKGHPAKR VFQALRIEVN SELLFLRTAL EKALDLLQVG GRIVVLSYHS 250 260 270 280 290 300 LEDRIVKHLF RSVSVSQLPK GFYINKDPEY KLIGKDLKNP KETEIANNPR ASSAHLRAVE 310 RVHSHSQAEA QIVEPRA

References

[1]

"Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome." Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M. Genome Res. 13:1800-1809(2003) [PubMed: 12902375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Twist.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014184 Genomic DNA. Translation: AAO44317.1.
RefSeq	NP_787348.1. NC_004572.3.
3D structure databases
ProteinModelPortal	Q83GN7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q83GN7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1089279.
GenomeReviews	Gene locus TWT_220 in contig AE014184_GR.
KEGG	twh:TWT220.
PATRIC	23996931. VBITroWhi72342_0349.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0275.
HOGENOM	HBG302779.
OMA	NPATRAF.
PhylomeDB	Q83GN7.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	TWHI203267:TW220-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_TROWT

Accession

Primary (citable) accession number: Q83GN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program