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Q83GH8 (DEF_TROWT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Peptide deformylase
Short name=PDF
EC=3.5.1.88
Alternative name(s):
Polypeptide deformylase
Gene names
Name:def
Ordered Locus Names:TWT_310
OrganismTropheryma whipplei (strain Twist) (Whipple's bacillus) [Complete proteome] [HAMAP]
Taxonomic identifier203267 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163

Catalytic activity

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163

Cofactor

Binds 1 Fe2+ ion By similarity. HAMAP MF_00163

Sequence similarities

Belongs to the polypeptide deformylase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandIron
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtranslation

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functioniron ion binding

Inferred from electronic annotation. Source: InterPro

peptide deformylase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 228228Peptide deformylase HAMAP MF_00163
PRO_0000082871

Sites

Active site1841 By similarity
Metal binding1411Iron By similarity
Metal binding1831Iron By similarity
Metal binding1871Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83GH8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: A02523288B7E07C6

FASTA22825,290
        10         20         30         40         50         60 
MSQDRRYTGC NTHSNTHSAQ DREKEGAVPK ISGGKILPIY ITGHAVLHAP AKPVTDFSGI 

        70         80         90        100        110        120 
QEIVRDMFAT MFAAPGVGLA GPQIGLGLRI FVYSYTEGDT LHQGVAINPD LLIPKGVPKR 

       130        140        150        160        170        180 
QTNKQQANNS TSCDEPDREG CLSFPGYQFP LERAPQVTLS AFDENKKPFT VHATGWLARI 

       190        200        210        220 
FQHEFDHLQG TLYVDRLAQK YSGEVRQAVL NNKWGIPGKY WVPQEPKE

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References

[1]"Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
Genome Res. 13:1800-1809(2003) [PubMed: 12902375] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Twist.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE014184 Genomic DNA. Translation: AAO44407.1.
RefSeqNP_787438.1. NC_004572.3.

3D structure databases

ProteinModelPortalQ83GH8.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ83GH8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1089563.
GenomeReviewsGene locus TWT_310 in contig AE014184_GR.
KEGGtwh:TWT310.
PATRIC23997121. VBITroWhi72342_0440.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0242.
HOGENOMHBG665227.
OMARITKVDE.
PhylomeDBQ83GH8.
ProtClustDBCLSK229782.

Enzyme and pathway databases

BioCycTWHI203267:TW310-MONOMER.

Family and domain databases

HAMAPMF_00163. Pep_deformylase.
[Tree]
InterProIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
Gene3DG3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KOK01462.
PANTHERPTHR10458. Fmet_deformylase. 1 hit.
PfamPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFPIRSF004749. Pep_def. 1 hit.
PRINTSPR01576. PDEFORMYLASE.
SUPFAMSSF56420. Fmet_deformylase. 1 hit.
TIGRFAMsTIGR00079. Pept_deformyl. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDEF_TROWT
AccessionPrimary (citable) accession number: Q83GH8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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