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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Tropheryma whipplei (strain Twist) (Whipple's bacillus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei574 – 5741ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTWHI203267:GJDK-410-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:TWT_385
OrganismiTropheryma whipplei (strain Twist) (Whipple's bacillus)
Taxonomic identifieri203267 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma
ProteomesiUP000002200 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 806806Leucine--tRNA ligasePRO_0000152111Add
BLAST

Proteomic databases

PRIDEiQ83GC6.

Interactioni

Protein-protein interaction databases

STRINGi203267.TWT385.

Structurei

3D structure databases

ProteinModelPortaliQ83GC6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi54 – 6411"HIGH" regionAdd
BLAST
Motifi571 – 5755"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83GC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHALRALDTL CENMEYNFRA LEEKWAPIWE RDRLFEVDEN DSETPRKYVL
60 70 80 90 100
DMFSYPSGDL HMGHAETYAY GDFIARYWRH RGYNVLHPVG WDSFGLPAEN
110 120 130 140 150
AAIKHGSDPK VWTYRNIDQQ ARSMRLYAAS FDWSRRLHTS DPEYYRWNQW
160 170 180 190 200
LFLKLYKHGL AYRKKAWVNW DPSDRTVLAN EQVLPDGTSE RSGALVVKKK
210 220 230 240 250
LTQWFLRITA YADRLLDDLS MLENNWPERV ITMQRNWIGR SEGVSIEFNI
260 270 280 290 300
PTLKRPVTVF TTRPETIFGV TYLALAFDSE VTEELASKSG VLGELLELRH
310 320 330 340 350
NIDKTHEGVR GLDLKSFAIH PLTGQSVPIF AASYILSDYA KGAVMSVPGH
360 370 380 390 400
DTRDERFAVR YNLPIVKIME DNRLISSGKY SGQSVTQARE NITRDLCAKS
410 420 430 440 450
LGRREISYRL RDWLISRQRY WGTPIPILYD SNGSEIPVEE DDLPVLLPDS
460 470 480 490 500
EGIDLTPSGL SPLGGIHDWV NLHKAGSLFR RDTDTMDTFF DSSWYFLRYL
510 520 530 540 550
NPDCDTAPFT LEKAKKWGPV DQYCGGVEHA VLHLLYARFI TKFLYDIGFV
560 570 580 590 600
DFKEPFLRLI NQGMVVLNGA KMSKSKGNIV EFSKEVSQHG VDVIRFALIF
610 620 630 640 650
SGPPEEDIDW KDVSMTGAAR FLSRCIQTAK EVPKRTADLS LGDIELRKHT
660 670 680 690 700
HSLLNDIDWL VDSYRFNVIA ARLMDLLNIT RKKIQTIGAD NPAIREAIET
710 720 730 740 750
IAIALDMFSP YTAEEMWEIL GNKYSVSKAL FPEVDTTFLE QKTTCAIVQI
760 770 780 790 800
DGRLRGRLNV LTNITTEQLV HSARSLPAIE HALSGRSVKR VICVPPKLVN

FVVEPK
Length:806
Mass (Da):92,300
Last modified:May 31, 2003 - v1
Checksum:i6A6153B7178A397B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014184 Genomic DNA. Translation: AAO44482.1.
RefSeqiNP_787513.1. NC_004572.3.

Genome annotation databases

EnsemblBacteriaiAAO44482; AAO44482; TWT_385.
GeneIDi1089449.
KEGGitwh:TWT385.
PATRICi23997291. VBITroWhi72342_0522.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014184 Genomic DNA. Translation: AAO44482.1.
RefSeqiNP_787513.1. NC_004572.3.

3D structure databases

ProteinModelPortaliQ83GC6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203267.TWT385.

Proteomic databases

PRIDEiQ83GC6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO44482; AAO44482; TWT_385.
GeneIDi1089449.
KEGGitwh:TWT385.
PATRICi23997291. VBITroWhi72342_0522.

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciTWHI203267:GJDK-410-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
    Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
    Genome Res. 13:1800-1809(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Twist.

Entry informationi

Entry nameiSYL_TROWT
AccessioniPrimary (citable) accession number: Q83GC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2004
Last sequence update: May 31, 2003
Last modified: March 31, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.