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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Tropheryma whipplei (strain Twist) (Whipple's bacillus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei109 – 1091Charge relay systemUniRule annotation
Active sitei184 – 1841Charge relay systemUniRule annotation
Active sitei208 – 2081Acyl-ester intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTWHI203267:GJDK-443-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:TWT_412
OrganismiTropheryma whipplei (strain Twist) (Whipple's bacillus)
Taxonomic identifieri203267 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesTropheryma
ProteomesiUP000002200 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Glutamyl-tRNA(Gln) amidotransferase subunit APRO_0000105226Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi203267.TWT412.

Structurei

3D structure databases

ProteinModelPortaliQ83GA1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0154.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiEOG61P6R9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83GA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRRAPFLIL LRFCRMGKMA CSGYPPFQGR IVLSEMPAHR LAALIRSKEV
60 70 80 90 100
SALEVAESFI DNIEASDSRI CAFLYTDFSY TRDVARRVDE ELKSATKLSP
110 120 130 140 150
LAGVPIAVKD MILTRDMPTT AGSKILEGWI PPYNATVIER ISRARMPILG
160 170 180 190 200
KTNQDEFGMG SSTEYSAYKT TRNPWDLSRT AGGSGGGSSA AVSASQAPLA
210 220 230 240 250
LGTDTGGSIR LPAHCTGTVG IRPTYGSVSR YGVIALASSF DQVGPCSSNI
260 270 280 290 300
LDAALLHEVI AGYDPADAVS IKDQDLNFSQ AAYEGANRGI SGVRLGFVNP
310 320 330 340 350
SNWCNSKITD LFGRTLKSLE SEGAVLHEVQ FPNFDHAVQA YYLIMQAEAS
360 370 380 390 400
SNLSRYDSIR FGPQEMAASA SGTVSKTRSI RFGPEVKRRI LLGTHILSAG
410 420 430 440 450
YYDDFYMSAQ KIRLLVKRDF AKIFSLVDVL LLPTAPTPAF KLGEKIDHHT
460 470 480 490 500
SMYKSDTATT PASLAGLPAG SIPMGVIDGL PVGLQIIAPG QFDSRVYSTG
510 520
AAIEQIIGDI HAMKNTKHNT GQTA
Length:524
Mass (Da):56,455
Last modified:June 1, 2003 - v1
Checksum:i925927E8D616705A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014184 Genomic DNA. Translation: AAO44509.1.
RefSeqiNP_787540.1. NC_004572.3.
WP_011102561.1. NC_004572.3.

Genome annotation databases

EnsemblBacteriaiAAO44509; AAO44509; TWT_412.
KEGGitwh:TWT412.
PATRICi23997359. VBITroWhi72342_0550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014184 Genomic DNA. Translation: AAO44509.1.
RefSeqiNP_787540.1. NC_004572.3.
WP_011102561.1. NC_004572.3.

3D structure databases

ProteinModelPortaliQ83GA1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203267.TWT412.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO44509; AAO44509; TWT_412.
KEGGitwh:TWT412.
PATRICi23997359. VBITroWhi72342_0550.

Phylogenomic databases

eggNOGiCOG0154.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiEOG61P6R9.

Enzyme and pathway databases

BioCyciTWHI203267:GJDK-443-MONOMER.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
    Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
    Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Twist.

Entry informationi

Entry nameiGATA_TROWT
AccessioniPrimary (citable) accession number: Q83GA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: June 24, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.