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Q83GA1 (GATA_TROWT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A

Short name=Glu-ADT subunit A
EC=6.3.5.7
Gene names
Name:gatA
Ordered Locus Names:TWT_412
OrganismTropheryma whipplei (strain Twist) (Whipple's bacillus) [Complete proteome] [HAMAP]
Taxonomic identifier203267 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120

Catalytic activity

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00120

Subunit structure

Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00120

Sequence similarities

Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 524524Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120
PRO_0000105226

Sites

Active site1091Charge relay system By similarity
Active site1841Charge relay system By similarity
Active site2081Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83GA1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 925927E8D616705A

FASTA52456,455
        10         20         30         40         50         60 
MIRRAPFLIL LRFCRMGKMA CSGYPPFQGR IVLSEMPAHR LAALIRSKEV SALEVAESFI 

        70         80         90        100        110        120 
DNIEASDSRI CAFLYTDFSY TRDVARRVDE ELKSATKLSP LAGVPIAVKD MILTRDMPTT 

       130        140        150        160        170        180 
AGSKILEGWI PPYNATVIER ISRARMPILG KTNQDEFGMG SSTEYSAYKT TRNPWDLSRT 

       190        200        210        220        230        240 
AGGSGGGSSA AVSASQAPLA LGTDTGGSIR LPAHCTGTVG IRPTYGSVSR YGVIALASSF 

       250        260        270        280        290        300 
DQVGPCSSNI LDAALLHEVI AGYDPADAVS IKDQDLNFSQ AAYEGANRGI SGVRLGFVNP 

       310        320        330        340        350        360 
SNWCNSKITD LFGRTLKSLE SEGAVLHEVQ FPNFDHAVQA YYLIMQAEAS SNLSRYDSIR 

       370        380        390        400        410        420 
FGPQEMAASA SGTVSKTRSI RFGPEVKRRI LLGTHILSAG YYDDFYMSAQ KIRLLVKRDF 

       430        440        450        460        470        480 
AKIFSLVDVL LLPTAPTPAF KLGEKIDHHT SMYKSDTATT PASLAGLPAG SIPMGVIDGL 

       490        500        510        520 
PVGLQIIAPG QFDSRVYSTG AAIEQIIGDI HAMKNTKHNT GQTA 

« Hide

References

[1]"Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Twist.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014184 Genomic DNA. Translation: AAO44509.1.
RefSeqNP_787540.1. NC_004572.3.

3D structure databases

ProteinModelPortalQ83GA1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203267.TWT412.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO44509; AAO44509; TWT_412.
GeneID1089420.
KEGGtwh:TWT412.
PATRIC23997359. VBITroWhi72342_0550.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0154.
KOK02433.
OMAMAMYLND.
OrthoDBEOG61P6R9.

Enzyme and pathway databases

BioCycTWHI203267:GJDK-443-MONOMER.

Family and domain databases

Gene3D3.90.1300.10. 1 hit.
HAMAPMF_00120. GatA.
InterProIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERPTHR11895. PTHR11895. 1 hit.
PfamPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMSSF75304. SSF75304. 1 hit.
TIGRFAMsTIGR00132. gatA. 1 hit.
PROSITEPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATA_TROWT
AccessionPrimary (citable) accession number: Q83GA1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families