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Q83G52

- SYI_TROWT

UniProt

Q83G52 - SYI_TROWT

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Tropheryma whipplei (strain Twist) (Whipple's bacillus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei635 – 6351ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciTWHI203267:GJDK-509-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:TWT_477
    OrganismiTropheryma whipplei (strain Twist) (Whipple's bacillus)
    Taxonomic identifieri203267 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma
    ProteomesiUP000002200: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10561056Isoleucine--tRNA ligasePRO_0000098571Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi203267.TWT477.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83G52.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi63 – 7311"HIGH" regionAdd
    BLAST
    Motifi632 – 6365"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q83G52-1 [UniParc]FASTAAdd to Basket

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    MCDQGEVSSQ NSSDYKEQRP TPRPNLPKIE ESVLAFWSSD KTFEASLEQR     50
    QHGKRWVFYD GPPFANGLPH FGHLLTGYIK DAIPRYQTMR GQYVPRVFGW 100
    DTHGLPAELE AMKRLGITEK SQIESMGIAS FNEAARKSVL TYVDQWEEYV 150
    NRQARWVDFK NGYKTLDLDY MESVLWAFKT LYKKGVIYEG YKVLPYCWND 200
    QTPLSNHELR MDDEVYKQRL DDSLTVTFPL IGQKAKTCGL DGVAALAWTT 250
    TPWTLPSNMA LIVSPNVEYV VVSSARQNSN SDFLLCKSSL DSYAECLGYE 300
    SGQDARASIR RTLLGKEIEG IHYKPLFDYY ADLHNAFTIL SDNYVDVTEG 350
    TGIVHASPAH GEDDKRVCDA FGVPTVVSIN DAACFTDVIS NYAGMHIFDA 400
    NAVIRSDLSR DGRILRHESY KHSYPHCWRC RSPLIYKAVT SWFFRITDSV 450
    NRMLELNQQI NWVPKSVKNG QFAKWLSSAK DWSISRTRYW GTPIPVWKSD 500
    NPEYPRIDCY GSLKELEDDF GIKLTDLHRP EIDRLTRPNP DDPTGASTMR 550
    RVPDVLDVWF DAASMPFAQL HYPFENIERF EANKSADFIV EYAGQIRGWF 600
    YLLHAMSTAL FDGVAFKNAI CHGIVLGDDG QKASKSLRNY PDVYDVFENE 650
    GSDAVRWYLI SSSILRGGSL IVSRKKIQDA IRQYITPLWS SWYFFHIYSE 700
    AARPGGYKAR FSVDSQDILD RYILSKTGLL VEDVTRFMDS FDMASAALQL 750
    RDFVAVLTNW YIRRSRDRFW DGSDTGAFDT LYTVLETLCR LGAVFVPMVS 800
    EHVYKCLTNS RSVHLSDWPD VATFPNETGL VETMDRVRKI CSTGLSLRKR 850
    LGIKARQPLS SAHIRVAQVG SLAQYKDIIS GELNVKTVSI EEGSCTQRML 900
    KILPRVAGPR LAGDVQTVIA AARRGDWTDH DGHVTAGGIP LLENEYQLVA 950
    GAQDSKNSQP LPFGGSVTLD TRIDETLRSE GVARDTVRQI QIARKEKDLN 1000
    ITDRISLEVC VPDEQVKNNL LAFSELICKE TLCDRLDILV KKGIDGITVS 1050
    LEKFRQ 1056
    Length:1,056
    Mass (Da):119,751
    Last modified:December 20, 2005 - v2
    Checksum:iD1D531A27E790967
    GO

    Sequence cautioni

    The sequence AAO44574.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014184 Genomic DNA. Translation: AAO44574.1. Different initiation.
    RefSeqiNP_787605.1. NC_004572.3.

    Genome annotation databases

    EnsemblBacteriaiAAO44574; AAO44574; TWT_477.
    GeneIDi1089357.
    KEGGitwh:TWT477.
    PATRICi23997503. VBITroWhi72342_0621.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014184 Genomic DNA. Translation: AAO44574.1 . Different initiation.
    RefSeqi NP_787605.1. NC_004572.3.

    3D structure databases

    ProteinModelPortali Q83G52.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203267.TWT477.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO44574 ; AAO44574 ; TWT_477 .
    GeneIDi 1089357.
    KEGGi twh:TWT477.
    PATRICi 23997503. VBITroWhi72342_0621.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci TWHI203267:GJDK-509-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
      Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
      Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Twist.

    Entry informationi

    Entry nameiSYI_TROWT
    AccessioniPrimary (citable) accession number: Q83G52
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3