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Q83G28 (DDL_TROWT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Synonyms:ddlA
Ordered Locus Names:TWT_505
OrganismTropheryma whipplei (strain Twist) (Whipple's bacillus) [Complete proteome] [HAMAP]
Taxonomic identifier203267 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeTropheryma

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 347347D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177900

Regions

Domain138 – 339202ATP-grasp
Nucleotide binding171 – 22656ATP By similarity

Sites

Metal binding2961Magnesium or manganese 1 By similarity
Metal binding3081Magnesium or manganese 1 By similarity
Metal binding3081Magnesium or manganese 2 By similarity
Metal binding3101Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83G28 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 126C40C59DCCF6EB

FASTA34738,729
        10         20         30         40         50         60 
MRRLLLLFGG RSQEHSVSYA SARALLEHIC DEWTVIPVGI TKHGDFVYCS TVSEQDYGEV 

        70         80         90        100        110        120 
IDNGNRVVWP SRAGCKKIEV VQARGKSFFV EFDLVFPLLH GVFGEDGTIQ GMLDLLDIPY 

       130        140        150        160        170        180 
VGSGVFASAA AMDKHYTKIL CSHAGIPVLP WYLIKGHAWH KNRDSFLKQI SDRFTFPLFV 

       190        200        210        220        230        240 
KPVDAGSSFG CTFVDFFEQL PVAIEHALQH GKSAIVEPAL DAPEVFCSLI ENNGVLQCSE 

       250        260        270        280        290        300 
LVFVKHSGSK FYDYSAKYLN PVEFVIPAQF DNTDGYAEIA SKVFFELGCR HYARIDFFVT 

       310        320        330        340 
ESGLVLNEIN TSPGLTQKSI FPSSFKSMQY SQVIETILAS AYCQVKR 

« Hide

References

[1]"Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome."
Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M.
Genome Res. 13:1800-1809(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Twist.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014184 Genomic DNA. Translation: AAO44602.1.
RefSeqNP_787633.1. NC_004572.3.

3D structure databases

ProteinModelPortalQ83G28.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203267.TWT505.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO44602; AAO44602; TWT_505.
GeneID1482489.
KEGGtwh:TWT505.
PATRIC23997571. VBITroWhi72342_0652.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
KOK01921.
OMAMEFCVLF.
OrthoDBEOG64BQ73.
ProtClustDBCLSK229604.

Enzyme and pathway databases

BioCycTWHI203267:GJDK-539-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_TROWT
AccessionPrimary (citable) accession number: Q83G28
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways