Q83FT2 (NTPA_TROWT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

TWT_621

Organism

Tropheryma whipplei (strain Twist) (Whipple's bacillus) [Complete proteome] [HAMAP]

Taxonomic identifier

203267 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Tropheryma

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178259
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	65 – 66	2	Substrate binding By similarity
Sites
	Metal binding	65	1	Magnesium or manganese By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83FT2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2E5370D8F58D27B6	FASTA	193	21,103
	10 20 30 40 50 60 MEIVFVSENE NKIAEAREIL LPLGFQSIFC GVTCRETGLT FTENAVLKAQ AAVGSVKDVP 70 80 90 100 110 120 IMADDSGICV DALNGMPGVL SSRWSQDGRN IDLLLWQMRD VPDVHRTAHF VCSIACVMPN 130 140 150 160 170 180 TEVRTVSSVW HGRILHAPDG TGGFGYDPVF LPDGYSVSAA GLGSDLKNRI SHRYKALRLM 190 SSLLKRTYFS CHA

References

[1]

"Tropheryma whipplei twist: a human pathogenic Actinobacteria with a reduced genome." Raoult D., Ogata H., Audic S., Robert C., Suhre K., Drancourt M., Claverie J.-M. Genome Res. 13:1800-1809(2003) [PubMed: 12902375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Twist.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014184 Genomic DNA. Translation: AAO44718.1.
RefSeq	NP_787749.1. NC_004572.3.
3D structure databases
ProteinModelPortal	Q83FT2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q83FT2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1482056.
GenomeReviews	Gene locus TWT_621 in contig AE014184_GR.
KEGG	twh:TWT621.
PATRIC	23997871. VBITroWhi72342_0796.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0127.
HOGENOM	HBG697237.
OMA	ESSTRLT.
PhylomeDB	Q83FT2.
ProtClustDB	CLSK230153.
Enzyme and pathway databases
BioCyc	TWHI203267:TW621-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_TROWT

Accession

Primary (citable) accession number: Q83FT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program