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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (CBU_0982)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ShikimateUniRule annotation1
Active sitei65Proton acceptorUniRule annotation1
Binding sitei102ShikimateUniRule annotation1
Binding sitei215NADP; via carbonyl oxygenUniRule annotation1
Binding sitei217ShikimateUniRule annotation1
Binding sitei239NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi127 – 131NADPUniRule annotation5
Nucleotide bindingi151 – 156NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciCBUR227377:G1G0B-13-MONOMER
UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:CBU_0010
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000212791 – 272Shikimate dehydrogenase (NADP(+))Add BLAST272

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_0010

Structurei

3D structure databases

ProteinModelPortaliQ83FD0
SMRiQ83FD0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 16Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237876
KOiK00014
OMAiFGNPIKH

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

Q83FD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKYAVIGNP VEHSLSPVIF QAFEKQTNHS FDYLKIKAPV NGFAAAVKKF
60 70 80 90 100
HDEGGKGANI TLPFKEEAYQ LADKRSQEAN EAHVASALQF REDGTIYAVN
110 120 130 140 150
YDGLGLVQDL TRNHNITLTQ KSILIVGAGG ATRGILGPLL NAAPEKIVIV
160 170 180 190 200
NRTPSKAHAL AKIFHLRGEI QGGGFDELEP MRYDVIIHAT SLGHQGKFPP
210 220 230 240 250
LPDGLIGSQS CCYDLSYGKI ASPFLQWAKD QGAKYNFDGL GMLVEHNAAV
260 270
FYLWFGIYPD TNPVIEMLQA HL
Length:272
Mass (Da):29,794
Last modified:June 1, 2003 - v1
Checksum:iF1DA0DAB51DADB73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA Translation: AAO89580.1
RefSeqiNP_819066.1, NC_002971.3
WP_010957324.1, NZ_LM994641.1

Genome annotation databases

EnsemblBacteriaiAAO89580; AAO89580; CBU_0010
GeneIDi1207872
KEGGicbu:CBU_0010
PATRICifig|227377.7.peg.12

Similar proteinsi

Entry informationi

Entry nameiAROE_COXBU
AccessioniPrimary (citable) accession number: Q83FD0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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