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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathway: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (CBU_0982)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611ShikimateUniRule annotation
Active sitei65 – 651Proton acceptorUniRule annotation
Binding sitei102 – 1021ShikimateUniRule annotation
Binding sitei215 – 2151NADP; via carbonyl oxygenUniRule annotation
Binding sitei217 – 2171ShikimateUniRule annotation
Binding sitei239 – 2391NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi127 – 1315NADPUniRule annotation
Nucleotide bindingi151 – 1566NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciCBUR227377:GJ7S-13-MONOMER.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:CBU_0010
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000002671 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 272272Shikimate dehydrogenase (NADP(+))PRO_1000021279Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_0010.

Structurei

3D structure databases

ProteinModelPortaliQ83FD0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 163Shikimate bindingUniRule annotation

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0169.
HOGENOMiHOG000237876.
KOiK00014.
OMAiSASQIPY.
OrthoDBiEOG6RRKMV.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83FD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKYAVIGNP VEHSLSPVIF QAFEKQTNHS FDYLKIKAPV NGFAAAVKKF
60 70 80 90 100
HDEGGKGANI TLPFKEEAYQ LADKRSQEAN EAHVASALQF REDGTIYAVN
110 120 130 140 150
YDGLGLVQDL TRNHNITLTQ KSILIVGAGG ATRGILGPLL NAAPEKIVIV
160 170 180 190 200
NRTPSKAHAL AKIFHLRGEI QGGGFDELEP MRYDVIIHAT SLGHQGKFPP
210 220 230 240 250
LPDGLIGSQS CCYDLSYGKI ASPFLQWAKD QGAKYNFDGL GMLVEHNAAV
260 270
FYLWFGIYPD TNPVIEMLQA HL
Length:272
Mass (Da):29,794
Last modified:June 1, 2003 - v1
Checksum:iF1DA0DAB51DADB73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO89580.1.
RefSeqiNP_819066.1. NC_002971.3.
WP_010957324.1. NZ_CCNR01000009.1.

Genome annotation databases

EnsemblBacteriaiAAO89580; AAO89580; CBU_0010.
GeneIDi1207872.
KEGGicbu:CBU_0010.
PATRICi17928699. VBICoxBur82552_0012.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO89580.1.
RefSeqiNP_819066.1. NC_002971.3.
WP_010957324.1. NZ_CCNR01000009.1.

3D structure databases

ProteinModelPortaliQ83FD0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227377.CBU_0010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO89580; AAO89580; CBU_0010.
GeneIDi1207872.
KEGGicbu:CBU_0010.
PATRICi17928699. VBICoxBur82552_0012.

Phylogenomic databases

eggNOGiCOG0169.
HOGENOMiHOG000237876.
KOiK00014.
OMAiSASQIPY.
OrthoDBiEOG6RRKMV.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BioCyciCBUR227377:GJ7S-13-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RSA 493 / Nine Mile phase I.

Entry informationi

Entry nameiAROE_COXBU
AccessioniPrimary (citable) accession number: Q83FD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: May 27, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.