Reviewed, UniProtKB/Swiss-Prot Q83FA4 (HEMH_COXBU)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	CBU_0042

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Ferrochelatase HAMAP MF_00323		PRO_0000175135
Sites
	Metal binding	204	1	Iron By similarity
	Metal binding	306	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83FA4-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: F2449F58B940F9AD	FASTA	354	40,554
	10 20 30 40 50 60 MAKQAIKRAK ILAVKNNNKI GVLLINLGTP DEPSVPAVRR YLRQFLSDPK VIDVPSLVRW 70 80 90 100 110 120 IIVHLCILPF RPKRSAKLYQ KIWMPEGSPL LVYSEMLRER VGETLGDDFC VALGMRYGKP 130 140 150 160 170 180 SIETALKKLQ EAQCRQLIVL PLFPQYSTST TASALEEVRA KNSFKEMTVI DRFFEEPHYI 190 200 210 220 230 240 DSMTTLIHEN LNEFQPDYFL FSYHGLPERH LVKSGCQLAI CNRKNNCSPI SSSNENCYRA 250 260 270 280 290 300 QCFETSRLIA KKLNLTDQQY GVAFQSRLGR AKWIEPYTDK YLIELSKKGI KKLMVVCPSF 310 320 330 340 350 PVDCLETLEE IGIRAQSQWQ RLDGETLKLI PSLNAHPQWV NAIAKMAKKS LQLF

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO89611.2.
RefSeq	NP_819097.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1207904.
GenomeReviews	Gene locus CBU_0042 in contig AE016828_GR.
KEGG	cbu:CBU_0042.
NMPDR	fig|227377.1.peg.41.
TIGR	CBU_0042.
Phylogenomic databases
HOGENOM	Q83FA4.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0042-MON.
BRENDA	4.99.1.1. 256353.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_COXBU

Accession

Primary (citable) accession number: Q83FA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	December 16, 2008
Last modified:	November 24, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)