Q83FA3 (NTPA_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

CBU_0043

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP-Rule MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP-Rule MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleoside triphosphate catabolic process Inferred from electronic annotation. Source: InterPro purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 200

200

Non-canonical purine NTP pyrophosphatase HAMAP-Rule MF_01405

PRO_0000178160

Regions

Region

8 – 13

6

Substrate binding By similarity

Region

69 – 70

2

Substrate binding By similarity

Sites

Metal binding

40

1

Magnesium or manganese By similarity

Metal binding

69

1

Magnesium or manganese By similarity

Binding site

157

1

Substrate By similarity

Binding site

177

1

Substrate By similarity

Binding site

183

1

Substrate By similarity

Secondary structure

1

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200

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Helix

11 – 20

10

Turn

21 – 23

3

Beta strand

24 – 30

7

Helix

31 – 34

4

Helix

46 – 61

16

Beta strand

65 – 74

10

Helix

75 – 77

3

Helix

82 – 84

3

Turn

85 – 89

5

Helix

95 – 109

15

Beta strand

115 – 127

13

Beta strand

134 – 144

11

Beta strand

151 – 153

3

Helix

157 – 159

3

Turn

163 – 165

3

Beta strand

166 – 168

3

Turn

169 – 171

3

Helix

174 – 180

7

Helix

182 – 196

15

Sequences

	Sequence		Length	Mass (Da)
	Q83FA3 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A35DF16EE7025113	FASTA	200	21,777
	10 20 30 40 50 60 MLEIVLASQN SSKLAEMQEL LRDLEIKFIP QTEFSVPDIE ETGSTFVENA IIKARHAAKQ 70 80 90 100 110 120 TGLPALADDS GLTIAALNSA PGVFSSRYAG KNATDAERIQ KVLEALEAAD DSDRSASFHC 130 140 150 160 170 180 VIALMENEND PAPLICHGVW EGEIAREPRG KNGFGYDPIF YVPSHQRTAA ELDPQEKNAI 190 200 SHRGQALEQL STVLTEAFLV

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE016828 Genomic DNA. Translation: AAO89612.1.

RefSeq

NP_819098.1. NC_002971.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3TQU	X-ray	1.90	A/B/C/D	1-200	[»]

ProteinModelPortal

Q83FA3.

SMR

Q83FA3. Positions 1-195.

ModBase

Search...

Protein-protein interaction databases

STRING

227377.CBU_0043.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAO89612; AAO89612; CBU_0043.

GeneID

1207905.

KEGG

cbu:CBU_0043.

PATRIC

17928763. VBICoxBur82552_0044.

Phylogenomic databases

eggNOG

COG0127.

HOGENOM

HOG000293319.

KO

K02428.

OMA

DGTLVWP.

ProtClustDB

PRK00120.

Enzyme and pathway databases

BioCyc

CBUR227377:GJ7S-47-MONOMER.

Family and domain databases

HAMAP

MF_01405. Non_canon_purine_NTPase.

InterPro

IPR002637. Ham1p-like.
IPR020922. Nucleoside-triphosphatase.
[Graphical view]

PANTHER

PTHR11067. PTHR11067. 1 hit.

Pfam

PF01725. Ham1p_like. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00042. TIGR00042. 1 hit.

ProtoNet

Search...

Entry information

Entry name

NTPA_COXBU

Accession

Primary (citable) accession number: Q83FA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program