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Protein

dITP/XTP pyrophosphatase

Gene

CBU_0043

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation
ITP + H2O = IMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40MagnesiumUniRule annotation1
Active sitei69Proton acceptorUniRule annotation1
Metal bindingi69MagnesiumUniRule annotation1
Binding sitei70Substrate; via amide nitrogenUniRule annotation1
Binding sitei177SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:CBU_0043
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001781601 – 200dITP/XTP pyrophosphataseAdd BLAST200

Proteomic databases

PRIDEiQ83FA3.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_0043.

Structurei

Secondary structure

1200
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi11 – 20Combined sources10
Turni21 – 23Combined sources3
Beta strandi24 – 30Combined sources7
Helixi31 – 34Combined sources4
Helixi46 – 61Combined sources16
Beta strandi65 – 74Combined sources10
Helixi75 – 77Combined sources3
Helixi82 – 84Combined sources3
Turni85 – 89Combined sources5
Helixi95 – 109Combined sources15
Beta strandi115 – 127Combined sources13
Beta strandi134 – 144Combined sources11
Beta strandi151 – 153Combined sources3
Helixi157 – 159Combined sources3
Turni163 – 165Combined sources3
Beta strandi166 – 168Combined sources3
Turni169 – 171Combined sources3
Helixi174 – 180Combined sources7
Helixi182 – 196Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TQUX-ray1.90A/B/C/D1-200[»]
ProteinModelPortaliQ83FA3.
SMRiQ83FA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni8 – 13Substrate bindingUniRule annotation6
Regioni154 – 157Substrate bindingUniRule annotation4
Regioni182 – 183Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
HOGENOMiHOG000293319.
KOiK02428.
OMAiYDPIFQP.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR020922. dITP/XTP_pyrophosphatase.
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83FA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEIVLASQN SSKLAEMQEL LRDLEIKFIP QTEFSVPDIE ETGSTFVENA
60 70 80 90 100
IIKARHAAKQ TGLPALADDS GLTIAALNSA PGVFSSRYAG KNATDAERIQ
110 120 130 140 150
KVLEALEAAD DSDRSASFHC VIALMENEND PAPLICHGVW EGEIAREPRG
160 170 180 190 200
KNGFGYDPIF YVPSHQRTAA ELDPQEKNAI SHRGQALEQL STVLTEAFLV
Length:200
Mass (Da):21,777
Last modified:June 1, 2003 - v1
Checksum:iA35DF16EE7025113
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO89612.1.
RefSeqiNP_819098.1. NC_002971.3.
WP_005769347.1. NC_002971.4.

Genome annotation databases

EnsemblBacteriaiAAO89612; AAO89612; CBU_0043.
GeneIDi1207905.
31484640.
KEGGicbu:CBU_0043.
PATRICifig|227377.7.peg.44.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIXTPA_COXBU
AccessioniPrimary (citable) accession number: Q83FA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2003
Last modified: June 7, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families