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Q83F67

- SYP_COXBU

UniProt

Q83F67 - SYP_COXBU

Protein

Proline--tRNA ligase

Gene

proS

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

    Catalytic activityi

    ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. proline-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. prolyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCBUR227377:GJ7S-81-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
    Alternative name(s):
    Prolyl-tRNA synthetaseUniRule annotation
    Short name:
    ProRSUniRule annotation
    Gene namesi
    Name:proSUniRule annotation
    Ordered Locus Names:CBU_0081
    OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
    Taxonomic identifieri227377 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
    ProteomesiUP000002671: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 566566Proline--tRNA ligasePRO_0000248680Add
    BLAST

    Proteomic databases

    PRIDEiQ83F67.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi227377.CBU_0081.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83F67.
    SMRiQ83F67. Positions 3-223.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0442.
    HOGENOMiHOG000076893.
    KOiK01881.
    OMAiMHQAYCN.
    OrthoDBiEOG6TTVMR.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPiMF_01569. Pro_tRNA_synth_type1.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q83F67-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLMKVSQFFL ATVKETPADA VLASHQLMIR AGMLRKLASG LYTWLPLGLR    50
    VLQKVADVVR EEMNRAGALE LLMPIVQPAS LWQESGRWEA YGAELLRIMD 100
    RHQNGFCFGP THEEVITDIA RQELKSYKQL PLNFYQIQTK FRDEIRPRFG 150
    VMRSREFLMK DAYSFDLDEK GMQAAYEKMF DAYRRIFTRL GLNFRAVLAD 200
    TGAIGGDYSH EFQVLADVGE DTVVYSDESD YAANIEKAAA QAPQGERVKP 250
    VAEMKKIATP GVRTIKQLAD KANILPEKGV KTLIVKGDES SLIALILRGD 300
    HELNDVKAQH LPGVAFPLQF ADEKEIREAI GCGPGSLGPV NLPIPFIVDR 350
    DAAQLVDFSC GANEDDFHWI NVNWERDVPL GSVADIRKVV EGDISPDGKG 400
    RLRFARGIEV GQVFQLGDKY SRKMNATVVD ELGKSRYLQM GCYGIGVSRT 450
    VAAAIEQNHD ERGIIWPMPM APFFIALVPV NMHKSYRVRE ACEKLYNELI 500
    DAGYEVLWDD RKERPGVMFA DMDLIGIPHR LVISESGLDR GIVEYKARKS 550
    KEAENVSLEN VLSVFR 566
    Length:566
    Mass (Da):63,589
    Last modified:June 1, 2003 - v1
    Checksum:i46EAB65336DC0383
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO89648.1.
    RefSeqiNP_819134.1. NC_002971.3.
    WP_010957364.1. NC_002971.3.

    Genome annotation databases

    EnsemblBacteriaiAAO89648; AAO89648; CBU_0081.
    GeneIDi1207951.
    KEGGicbu:CBU_0081.
    PATRICi17928841. VBICoxBur82552_0083.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO89648.1 .
    RefSeqi NP_819134.1. NC_002971.3.
    WP_010957364.1. NC_002971.3.

    3D structure databases

    ProteinModelPortali Q83F67.
    SMRi Q83F67. Positions 3-223.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 227377.CBU_0081.

    Proteomic databases

    PRIDEi Q83F67.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO89648 ; AAO89648 ; CBU_0081 .
    GeneIDi 1207951.
    KEGGi cbu:CBU_0081.
    PATRICi 17928841. VBICoxBur82552_0083.

    Phylogenomic databases

    eggNOGi COG0442.
    HOGENOMi HOG000076893.
    KOi K01881.
    OMAi MHQAYCN.
    OrthoDBi EOG6TTVMR.

    Enzyme and pathway databases

    BioCyci CBUR227377:GJ7S-81-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPi MF_01569. Pro_tRNA_synth_type1.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view ]
    PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view ]
    PRINTSi PR01046. TRNASYNTHPRO.
    SUPFAMi SSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RSA 493 / Nine Mile phase I.

    Entry informationi

    Entry nameiSYP_COXBU
    AccessioniPrimary (citable) accession number: Q83F67
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Coxiella burnetii
      Coxiella burnetii (strain RSA 493): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3