Q83F35 (RSMH_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	CBU_0116

Organism

Coxiella burnetii

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000108616
Regions
	Region	32 – 34	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	52	1	S-adenosyl-L-methionine By similarity
	Binding site	78	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	100	1	S-adenosyl-L-methionine By similarity
	Binding site	107	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83F35 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 836DCCF0D6BF77DE	FASTA	307	34,923
	10 20 30 40 50 60 MEAHKPVLFD EVMEGLAIRP DGIYVDGTFG RGGHSFGILQ RLGPNGRLMA MDKDPDAVAV 70 80 90 100 110 120 ANKALFEDAR FSIVHETFAN LQKAVRDRGW EGKVNGILLD IGVSSPQLED AKRGFSFSKD 130 140 150 160 170 180 GPLDMRMNPK QSMDAASWIN QAAMEDIRRV LWNYGEERFA KRIAQAIVNA REEKPITRTQ 190 200 210 220 230 240 ELSDIVIKAY PQREIKKHPA TRTFQAIRIF INRELDELRE CLPQCLETLA VGGRLCVISF 250 260 270 280 290 300 HSLEDRLVKR FIQKESRDHL PREIPILAKD IKHRLKPLGS LIRPTEAEIK KNPRARSARL RIVEKLS

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO89680.1.
RefSeq	NP_819166.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83F35.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1207987.
GenomeReviews	Gene locus CBU_0116 in contig AE016828_GR.
KEGG	cbu:CBU_0116.
NMPDR	fig|227377.1.peg.110.
PATRIC	17928917. VBICoxBur82552_0119.
TIGR	CBU_0116.
Phylogenomic databases
HOGENOM	HBG302779.
OMA	NPATRAF.
PhylomeDB	Q83F35.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0116-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_COXBU

Accession

Primary (citable) accession number: Q83F35

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program