Reviewed, UniProtKB/Swiss-Prot Q83F20 (MURD_COXBU)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	CBU_0131

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000109006
Regions
	Nucleotide binding	113 – 119	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q83F20-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2B15F09C8E53E2A6	FASTA	442	48,773
	10 20 30 40 50 60 MSSESLTVIV GLGKTGLSCA QFLAAKNQPF AVMDSREEPP EWENFIKTYP RVELIRGQFS 70 80 90 100 110 120 EKLLNEAQEI ILSPGVSLQE PLIAKQAAQG KSIIGDIELF ARNVNKPIIA ITGSNGKTTV 130 140 150 160 170 180 TTVVGLMMKA AGRNVSVCGN IGEPVLEQIT PEPDYYVLEL SSFQLETTFS LRSQAATILN 190 200 210 220 230 240 ISEDHMNRYA TLQDYLRAKQ RIYTDCFIPI VNADEPEIWR HLPFNKKPLS FGLNNAADFS 250 260 270 280 290 300 LAEHNQKTSI AYQGKILMPI QELKLNARHH LQNALAALAL GTAAKIPIEN MLHVLRDFSG 310 320 330 340 350 360 IRHRCQWVRK YKEIDYYNDS KGTNVGATRA AIESLGQAAK GQLILIAGGQ GKGADFSPLK 370 380 390 400 410 420 DVVKRYVKQV ILIGEDAPLL EKTLKEITVI KHADSMNEAV KRSTQAAKAG DIVLLSPACA 430 440 SFDMFTNYEH RGDVFTETVE AL

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO89695.1.
RefSeq	NP_819181.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1208002.
GenomeReviews	Gene locus CBU_0131 in contig AE016828_GR.
KEGG	cbu:CBU_0131.
NMPDR	fig|227377.1.peg.125.
TIGR	CBU_0131.
Phylogenomic databases
HOGENOM	Q83F20.
OMA	VKYYNDS
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0131-MON.
BRENDA	6.3.2.9. 256353.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_COXBU

Accession

Primary (citable) accession number: Q83F20

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2003
Last modified:	November 24, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)