Reviewed, UniProtKB/Swiss-Prot Q83F16 (MURB_COXBU)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	CBU_0137

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179205
Regions
	Domain	27 – 225	199	FAD-binding PCMH-type
Sites
	Active site	190	1	By similarity
	Active site	239	1	Proton donor By similarity
	Active site	309	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83F16-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 645EEF641A099AB6	FASTA	316	34,632
	10 20 30 40 50 60 MDKENFTRLR GELFCDHPLA RYTSWRVGGK AERFYRPADL FDLQDFLTQL PSDEPLTWLG 70 80 90 100 110 120 LGSNVLIRDG GIKGTVILTL NRLKELSVVN SQLVFREKSG TEDFFSGNGK TIIRAEAGVT 130 140 150 160 170 180 CAKLAKFCVS QGLEDGAFFA GIPGTVGGAL AMNAGAFGGE TWRTVIGVET MNHQGEILKR 190 200 210 220 230 240 TPDEFKIHYR QVEGLENQFF IAGYFCFNHG DPDKAKTAIN ALLKKRNLSQ PIGKYSCGSV 250 260 270 280 290 300 FRNPPGDYAA RLIESAGLKG KSIGNAEVSE KHANFILNKG NASAADIEAL IHYVAQHVSQ 310 IHGIQLVKEV HIIGRS

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO89701.1.
RefSeq	NP_819187.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1208008.
GenomeReviews	Gene locus CBU_0137 in contig AE016828_GR.
KEGG	cbu:CBU_0137.
NMPDR	fig|227377.1.peg.131.
TIGR	CBU_0137.
Phylogenomic databases
HOGENOM	Q83F16.
OMA	QPIREKT
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0137-MON.
BRENDA	1.1.1.158. 256353.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_COXBU

Accession

Primary (citable) accession number: Q83F16

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	June 1, 2003
Last modified:	November 24, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)