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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei185Thioimide intermediateUniRule annotation1
Active sitei192Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi89 – 90NADPHUniRule annotation2
Nucleotide bindingi253 – 254NADPHUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:CBU_0151
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001630281 – 278NADPH-dependent 7-cyano-7-deazaguanine reductaseAdd BLAST278

Proteomic databases

PRIDEiQ83F02.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_0151.

Structurei

3D structure databases

ProteinModelPortaliQ83F02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 89Substrate bindingUniRule annotation3
Regioni224 – 225Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R1K. Bacteria.
COG0780. LUCA.
COG2904. LUCA.
HOGENOMiHOG000273755.
KOiK06879.
OMAiQAFELSW.

Family and domain databases

HAMAPiMF_00817. QueF_type2. 1 hit.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83F02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLRVLHEK SELGKTTVYP KEYAPHLLLP IPRDLNRKTL NVNVSEPPPF
60 70 80 90 100
YGYDLWNAYE LSWLNEKGKP FAARGEFIIP ATSSHLIESK SFKLYLNSFN
110 120 130 140 150
NERFADAAAV SQTMKRDLSK RVNESVTVNF ILHETEIPVA YSPKGSLLDV
160 170 180 190 200
LDIAIDTYSP DPNLLSTSQE TVTETLYSHL LKSNCPVTGQ PDWGSIEIHY
210 220 230 240 250
TGPKIDHVQL LKYIISYRNH EEFHEACVER FFMDILRHCR PQELTVQARY
260 270
TRRGGLDINP YRSTNPTFSV QNHRSFRQ
Length:278
Mass (Da):32,018
Last modified:June 1, 2003 - v1
Checksum:i10530DB1A19EF396
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO89715.1.
RefSeqiNP_819201.1. NC_002971.3.
WP_010957407.1. NZ_CCYB01000063.1.

Genome annotation databases

EnsemblBacteriaiAAO89715; AAO89715; CBU_0151.
GeneIDi1208022.
KEGGicbu:CBU_0151.
PATRICi17928983. VBICoxBur82552_0152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO89715.1.
RefSeqiNP_819201.1. NC_002971.3.
WP_010957407.1. NZ_CCYB01000063.1.

3D structure databases

ProteinModelPortaliQ83F02.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227377.CBU_0151.

Proteomic databases

PRIDEiQ83F02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO89715; AAO89715; CBU_0151.
GeneIDi1208022.
KEGGicbu:CBU_0151.
PATRICi17928983. VBICoxBur82552_0152.

Phylogenomic databases

eggNOGiENOG4107R1K. Bacteria.
COG0780. LUCA.
COG2904. LUCA.
HOGENOMiHOG000273755.
KOiK06879.
OMAiQAFELSW.

Enzyme and pathway databases

UniPathwayiUPA00392.

Family and domain databases

HAMAPiMF_00817. QueF_type2. 1 hit.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUEF_COXBU
AccessioniPrimary (citable) accession number: Q83F02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.