Q83ES9 (RS12_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
30S ribosomal protein S12

Gene names

Name:	rpsL
Ordered Locus Names:	CBU_0233

Organism

Coxiella burnetii

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	With S4 and S5 plays an important role in translational accuracy By similarity. HAMAP MF_00403_B Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit By similarity. HAMAP MF_00403_B
Subunit structure	Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex By similarity.
Sequence similarities	Belongs to the ribosomal protein S12P family.
Caution	Because the enzyme that would modify Asp-89 to 3-methylthioaspartic acid has not been found in the proteome of this organism, that modification is not predicted.
Sequence caution	The sequence AAO89791.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding tRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 124	124	30S ribosomal protein S12 HAMAP MF_00403_B		PRO_0000146215

Sequences

	Sequence		Length	Mass (Da)
	Q83ES9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: CB4EB41201253A9D	FASTA	124	13,753
	10 20 30 40 50 60 MARINQLVRK PRRARAKKSD VPALEGCPQR RGVCTRVYTT TPKKPNSALR KVARVRITNG 70 80 90 100 110 120 AEVTAYIGGE GHNLQEHSVV LIRGGRVKDL PGVRYHIVRG SLDTAGVSGR RRGRSKYGEK KPKE

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO89791.2. Different initiation.
RefSeq	NP_819277.2. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83ES9.
SMR	Q83ES9. Positions 2-123.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1208114.
GenomeReviews	Gene locus CBU_0233 in contig AE016828_GR.
KEGG	cbu:CBU_0233.
NMPDR	fig|227377.1.peg.221.
PATRIC	17929159. VBICoxBur82552_0228.
TIGR	CBU_0233.
Phylogenomic databases
HOGENOM	HBG747181.
OMA	KEVNAYI.
PhylomeDB	Q83ES9.
ProtClustDB	PRK05163.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0233-MONOMER.
Family and domain databases
HAMAP	MF_00403_B. Ribosomal_S12_B. [Tree]
InterPro	IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR006032. Ribosomal_S12/S23. IPR005679. Ribosomal_S12_bac. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K02950.
PANTHER	PTHR11652. Ribosomal_S12_23. 1 hit.
Pfam	PF00164. Ribosomal_S12. 1 hit. [Graphical view]
PIRSF	PIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTS	PR01034. RIBOSOMALS12.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00981. RpsL_bact. 1 hit.
PROSITE	PS00055. RIBOSOMAL_S12. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RS12_COXBU

Accession

Primary (citable) accession number: Q83ES9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program