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Q83ES6

- EFTU_COXBU

UniProt

Q83ES6 - EFTU_COXBU

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Protein

Elongation factor Tu

Gene

tufA

more
Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi81 – 855GTPUniRule annotation
Nucleotide bindingi136 – 1394GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: InterPro
  2. GTP binding Source: UniProtKB-HAMAP
  3. translation elongation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBUR227377:GJ7S-228-MONOMER.
CBUR227377:GJ7S-242-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufAUniRule annotation
Synonyms:tuf-1
Ordered Locus Names:CBU_0221.2
ORF Names:CBU_0221b
AND
Name:tufBUniRule annotation
Synonyms:tuf-2
Ordered Locus Names:CBU_0236
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000002671: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397Elongation factor TuPRO_1000015645Add
BLAST

Proteomic databases

PRIDEiQ83ES6.

Expressioni

Developmental stagei

Found in the large cell variant (LCV) stage, undetectable in the small cell variant (SCV) stage; at protein level. LCVs are more metabolically active than SCVs.2 Publications

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_0236.

Structurei

3D structure databases

ProteinModelPortaliQ83ES6.
SMRiQ83ES6. Positions 2-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 206197tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 268G1By similarity
Regioni60 – 645G2By similarity
Regioni81 – 844G3By similarity
Regioni136 – 1394G4By similarity
Regioni174 – 1763G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
KOiK02358.
OMAiGTEMCMP.
OrthoDBiEOG6R5C6X.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83ES6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKEKFVREK PHVNVGTIGH VDHGKTTLTA ALTKVLSEKY GGEKKAFDQI
60 70 80 90 100
DNAPEERARG ITIATSHVEY QSDKRHYAHV DCPGHADYVK NMITGAAQMD
110 120 130 140 150
GAILVVSAAD GPMPQTREHI VLAKQVGVPN IVVYLNKADM VDDKELLELV
160 170 180 190 200
EMEVRDLLNS YDFPGDETPI IVGSALKALE GDKSEVGEPS IIKLVETMDT
210 220 230 240 250
YFPQPERAID KPFLMPIEDV FSISGRGTVV TGRVERGIIK VGDEIEIVGI
260 270 280 290 300
KDTTKTTCTG VEMFRKLLDE GQAGDNVGIL LRGTKREEVE RGQVLAKPGS
310 320 330 340 350
ITPHKKFEAE IYVLSKEEGG RHTPFLQGYR PQFYFRTTDV TGQLLSLPEG
360 370 380 390
IEMVMPGDNV KVTVELIAPV AMDEGLRFAV REGGRTVGAG VVTKIIE
Length:397
Mass (Da):43,526
Last modified:June 1, 2003 - v1
Checksum:i8157B2CD749064FF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016828 Genomic DNA. Translation: ACI15245.1.
AE016828 Genomic DNA. Translation: AAO89794.1.
AF136604 Genomic DNA. Translation: AAD32649.1.
RefSeqiNP_819280.1. NC_002971.3.
YP_002332954.1. NC_002971.3.

Genome annotation databases

EnsemblBacteriaiAAO89794; AAO89794; CBU_0236.
ACI15245; ACI15245; CBU_0221b.
GeneIDi1208103.
1208117.
KEGGicbu:CBU_0221b.
cbu:CBU_0236.
PATRICi17929135. VBICoxBur82552_0217.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016828 Genomic DNA. Translation: ACI15245.1 .
AE016828 Genomic DNA. Translation: AAO89794.1 .
AF136604 Genomic DNA. Translation: AAD32649.1 .
RefSeqi NP_819280.1. NC_002971.3.
YP_002332954.1. NC_002971.3.

3D structure databases

ProteinModelPortali Q83ES6.
SMRi Q83ES6. Positions 2-396.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 227377.CBU_0236.

Proteomic databases

PRIDEi Q83ES6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO89794 ; AAO89794 ; CBU_0236 .
ACI15245 ; ACI15245 ; CBU_0221b .
GeneIDi 1208103.
1208117.
KEGGi cbu:CBU_0221b.
cbu:CBU_0236.
PATRICi 17929135. VBICoxBur82552_0217.

Phylogenomic databases

eggNOGi COG0050.
HOGENOMi HOG000229290.
KOi K02358.
OMAi GTEMCMP.
OrthoDBi EOG6R5C6X.

Enzyme and pathway databases

BioCyci CBUR227377:GJ7S-228-MONOMER.
CBUR227377:GJ7S-242-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_00118_B. EF_Tu_B.
InterProi IPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RSA 493 / Nine Mile phase I.
  2. "Differential expression of translational elements by life cycle variants of Coxiella burnetii."
    Seshadri R., Hendrix L.R., Samuel J.E.
    Infect. Immun. 67:6026-6033(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-358, DEVELOPMENTAL STAGE.
    Strain: RSA 493 / Nine Mile phase I.
  3. "Proteome and antigen profiling of Coxiella burnetii developmental forms."
    Coleman S.A., Fischer E.R., Cockrell D.C., Voth D.E., Howe D., Mead D.J., Samuel J.E., Heinzen R.A.
    Infect. Immun. 75:290-298(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DEVELOPMENTAL STAGE.
    Strain: Nine Mile Crazy / RSA 514.

Entry informationi

Entry nameiEFTU_COXBU
AccessioniPrimary (citable) accession number: Q83ES6
Secondary accession number(s): B5QS64, Q83ET7, Q9X6G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3