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Q83ES6 (EFTU_COXBU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongation factor Tu

Short name=EF-Tu
Gene names
Name:tufA
Synonyms:tuf-1
Ordered Locus Names:CBU_0221.2
ORF Names:CBU_0221b
AND
Name:tufB
Synonyms:tuf-2
Ordered Locus Names:CBU_0236
OrganismCoxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]
Taxonomic identifier227377 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. HAMAP-Rule MF_00118

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00118

Subcellular location

Cytoplasm HAMAP-Rule MF_00118.

Developmental stage

Found in the large cell variant (LCV) stage, undetectable in the small cell variant (SCV) stage; at protein level. LCVs are more metabolically active than SCVs. Ref.2 Ref.3

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionElongation factor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

GTPase activity

Inferred from electronic annotation. Source: InterPro

translation elongation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 397397Elongation factor Tu HAMAP-Rule MF_00118
PRO_1000015645

Regions

Nucleotide binding19 – 268GTP By similarity
Nucleotide binding81 – 855GTP By similarity
Nucleotide binding136 – 1394GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83ES6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 8157B2CD749064FF

FASTA39743,526
        10         20         30         40         50         60 
MSKEKFVREK PHVNVGTIGH VDHGKTTLTA ALTKVLSEKY GGEKKAFDQI DNAPEERARG 

        70         80         90        100        110        120 
ITIATSHVEY QSDKRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSAAD GPMPQTREHI 

       130        140        150        160        170        180 
VLAKQVGVPN IVVYLNKADM VDDKELLELV EMEVRDLLNS YDFPGDETPI IVGSALKALE 

       190        200        210        220        230        240 
GDKSEVGEPS IIKLVETMDT YFPQPERAID KPFLMPIEDV FSISGRGTVV TGRVERGIIK 

       250        260        270        280        290        300 
VGDEIEIVGI KDTTKTTCTG VEMFRKLLDE GQAGDNVGIL LRGTKREEVE RGQVLAKPGS 

       310        320        330        340        350        360 
ITPHKKFEAE IYVLSKEEGG RHTPFLQGYR PQFYFRTTDV TGQLLSLPEG IEMVMPGDNV 

       370        380        390 
KVTVELIAPV AMDEGLRFAV REGGRTVGAG VVTKIIE 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii."
Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. expand/collapse author list , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F.
Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RSA 493 / Nine Mile phase I.
[2]"Differential expression of translational elements by life cycle variants of Coxiella burnetii."
Seshadri R., Hendrix L.R., Samuel J.E.
Infect. Immun. 67:6026-6033(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-358, DEVELOPMENTAL STAGE.
Strain: RSA 493 / Nine Mile phase I.
[3]"Proteome and antigen profiling of Coxiella burnetii developmental forms."
Coleman S.A., Fischer E.R., Cockrell D.C., Voth D.E., Howe D., Mead D.J., Samuel J.E., Heinzen R.A.
Infect. Immun. 75:290-298(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DEVELOPMENTAL STAGE.
Strain: Nine Mile Crazy / RSA 514.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016828 Genomic DNA. Translation: ACI15245.1.
AE016828 Genomic DNA. Translation: AAO89794.1.
AF136604 Genomic DNA. Translation: AAD32649.1.
RefSeqNP_819280.1. NC_002971.3.
YP_002332954.1. NC_002971.3.

3D structure databases

ProteinModelPortalQ83ES6.
SMRQ83ES6. Positions 2-396.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227377.CBU_0236.

Proteomic databases

PRIDEQ83ES6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO89794; AAO89794; CBU_0236.
ACI15245; ACI15245; CBU_0221b.
GeneID1208103.
1208117.
KEGGcbu:CBU_0221b.
cbu:CBU_0236.
PATRIC17929135. VBICoxBur82552_0217.

Phylogenomic databases

eggNOGCOG0050.
HOGENOMHOG000229290.
KOK02358.
OMAVTPHTEF.
OrthoDBEOG6R5C6X.
ProtClustDBPRK00049.

Enzyme and pathway databases

BioCycCBUR227377:GJ7S-228-MONOMER.
CBUR227377:GJ7S-242-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
HAMAPMF_00118_B. EF_Tu_B.
InterProIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEFTU_COXBU
AccessionPrimary (citable) accession number: Q83ES6
Secondary accession number(s): B5QS64, Q83ET7, Q9X6G1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Coxiella burnetii

Coxiella burnetii (strain RSA 493): entries and gene names