Reviewed, UniProtKB/Swiss-Prot Q83ES6 (EFTU_COXBU)

Names and origin

Protein names

Recommended name:
    Elongation factor Tu
      Short name=EF-Tu

Gene names

Name:	tufA
Synonyms:	tuf-1
Ordered Locus Names:	CBU_0221.2
ORF Names:	CBU_0221b
AND
Name:	tufB
Synonyms:	tuf-2
Ordered Locus Names:	CBU_0236

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. HAMAP MF_00118
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Developmental stage	Found in the large cell variant (LCV) stage, undetectable in the small cell variant (SCV) stage; at protein level. LCVs are more metabolically active than SCVs. Ref.2 Ref.3
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translational elongation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Elongation factor Tu HAMAP MF_00118		PRO_1000015645
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	136 – 139	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83ES6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8157B2CD749064FF	FASTA	397	43,526
	10 20 30 40 50 60 MSKEKFVREK PHVNVGTIGH VDHGKTTLTA ALTKVLSEKY GGEKKAFDQI DNAPEERARG 70 80 90 100 110 120 ITIATSHVEY QSDKRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSAAD GPMPQTREHI 130 140 150 160 170 180 VLAKQVGVPN IVVYLNKADM VDDKELLELV EMEVRDLLNS YDFPGDETPI IVGSALKALE 190 200 210 220 230 240 GDKSEVGEPS IIKLVETMDT YFPQPERAID KPFLMPIEDV FSISGRGTVV TGRVERGIIK 250 260 270 280 290 300 VGDEIEIVGI KDTTKTTCTG VEMFRKLLDE GQAGDNVGIL LRGTKREEVE RGQVLAKPGS 310 320 330 340 350 360 ITPHKKFEAE IYVLSKEEGG RHTPFLQGYR PQFYFRTTDV TGQLLSLPEG IEMVMPGDNV 370 380 390 KVTVELIAPV AMDEGLRFAV REGGRTVGAG VVTKIIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.
[2]	"Differential expression of translational elements by life cycle variants of Coxiella burnetii." Seshadri R., Hendrix L.R., Samuel J.E. Infect. Immun. 67:6026-6033(1999) [PubMed: 10531263] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-358, DEVELOPMENTAL STAGE. Strain: Nine Mile phase I / RSA 493.
[3]	"Proteome and antigen profiling of Coxiella burnetii developmental forms." Coleman S.A., Fischer E.R., Cockrell D.C., Voth D.E., Howe D., Mead D.J., Samuel J.E., Heinzen R.A. Infect. Immun. 75:290-298(2007) [PubMed: 17088354] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DEVELOPMENTAL STAGE. Strain: Nine Mile Crazy / RSA 514.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: ACI15245.1. AE016828 Genomic DNA. Translation: AAO89794.1. AF136604 Genomic DNA. Translation: AAD32649.1.
RefSeq	NP_819280.1. YP_002332954.1.
3D structure databases
HSSP	HSSP built from PDB template 1XB2 based on UniProtKB P49410.
SMR	Q83ES6. Positions 4-396.
ModBase	Search...
Genome annotation databases
GeneID	1208103. 1208117.
GenomeReviews	Gene locus CBU_0221.2 in contig AE016828_GR. Gene locus CBU_0221b in contig AE016828_GR.
KEGG	cbu:CBU_0236.
NMPDR	fig|227377.1.peg.224.
TIGR	CBU_0236.
Phylogenomic databases
HOGENOM	Q83ES6.
OMA	LMEAVDN
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0236-MON.
Family and domain databases
HAMAP	MF_00118. [Tree]
InterPro	IPR000795. ProtSyn_GTP_bd. IPR005225. Small_GTP_bd. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF31. Transl_elong_EFTu/EF1A_bac/org. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_COXBU

Accession

Primary (citable) accession number: Q83ES6
Secondary accession number(s): B5QS64, Q83ET7, Q9X6G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 1, 2003
Last modified:	November 24, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)