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Q83ES6

- EFTU_COXBU

UniProt

Q83ES6 - EFTU_COXBU

Protein

Elongation factor Tu

Gene

tufA

more
Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 268GTPUniRule annotation
    Nucleotide bindingi81 – 855GTPUniRule annotation
    Nucleotide bindingi136 – 1394GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: InterPro
    2. GTP binding Source: UniProtKB-HAMAP
    3. translation elongation factor activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Elongation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCBUR227377:GJ7S-228-MONOMER.
    CBUR227377:GJ7S-242-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor TuUniRule annotation
    Short name:
    EF-TuUniRule annotation
    Gene namesi
    Name:tufAUniRule annotation
    Synonyms:tuf-1
    Ordered Locus Names:CBU_0221.2
    ORF Names:CBU_0221b
    AND
    Name:tufBUniRule annotation
    Synonyms:tuf-2
    Ordered Locus Names:CBU_0236
    OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
    Taxonomic identifieri227377 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
    ProteomesiUP000002671: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 397397Elongation factor TuPRO_1000015645Add
    BLAST

    Proteomic databases

    PRIDEiQ83ES6.

    Expressioni

    Developmental stagei

    Found in the large cell variant (LCV) stage, undetectable in the small cell variant (SCV) stage; at protein level. LCVs are more metabolically active than SCVs.2 Publications

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi227377.CBU_0236.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83ES6.
    SMRiQ83ES6. Positions 2-396.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 206197tr-type GAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni19 – 268G1By similarity
    Regioni60 – 645G2By similarity
    Regioni81 – 844G3By similarity
    Regioni136 – 1394G4By similarity
    Regioni174 – 1763G5By similarity

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0050.
    HOGENOMiHOG000229290.
    KOiK02358.
    OMAiGTEMCMP.
    OrthoDBiEOG6R5C6X.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00118_B. EF_Tu_B.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q83ES6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKEKFVREK PHVNVGTIGH VDHGKTTLTA ALTKVLSEKY GGEKKAFDQI    50
    DNAPEERARG ITIATSHVEY QSDKRHYAHV DCPGHADYVK NMITGAAQMD 100
    GAILVVSAAD GPMPQTREHI VLAKQVGVPN IVVYLNKADM VDDKELLELV 150
    EMEVRDLLNS YDFPGDETPI IVGSALKALE GDKSEVGEPS IIKLVETMDT 200
    YFPQPERAID KPFLMPIEDV FSISGRGTVV TGRVERGIIK VGDEIEIVGI 250
    KDTTKTTCTG VEMFRKLLDE GQAGDNVGIL LRGTKREEVE RGQVLAKPGS 300
    ITPHKKFEAE IYVLSKEEGG RHTPFLQGYR PQFYFRTTDV TGQLLSLPEG 350
    IEMVMPGDNV KVTVELIAPV AMDEGLRFAV REGGRTVGAG VVTKIIE 397
    Length:397
    Mass (Da):43,526
    Last modified:June 1, 2003 - v1
    Checksum:i8157B2CD749064FF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: ACI15245.1.
    AE016828 Genomic DNA. Translation: AAO89794.1.
    AF136604 Genomic DNA. Translation: AAD32649.1.
    RefSeqiNP_819280.1. NC_002971.3.
    YP_002332954.1. NC_002971.3.

    Genome annotation databases

    EnsemblBacteriaiAAO89794; AAO89794; CBU_0236.
    ACI15245; ACI15245; CBU_0221b.
    GeneIDi1208103.
    1208117.
    KEGGicbu:CBU_0221b.
    cbu:CBU_0236.
    PATRICi17929135. VBICoxBur82552_0217.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: ACI15245.1 .
    AE016828 Genomic DNA. Translation: AAO89794.1 .
    AF136604 Genomic DNA. Translation: AAD32649.1 .
    RefSeqi NP_819280.1. NC_002971.3.
    YP_002332954.1. NC_002971.3.

    3D structure databases

    ProteinModelPortali Q83ES6.
    SMRi Q83ES6. Positions 2-396.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 227377.CBU_0236.

    Proteomic databases

    PRIDEi Q83ES6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO89794 ; AAO89794 ; CBU_0236 .
    ACI15245 ; ACI15245 ; CBU_0221b .
    GeneIDi 1208103.
    1208117.
    KEGGi cbu:CBU_0221b.
    cbu:CBU_0236.
    PATRICi 17929135. VBICoxBur82552_0217.

    Phylogenomic databases

    eggNOGi COG0050.
    HOGENOMi HOG000229290.
    KOi K02358.
    OMAi GTEMCMP.
    OrthoDBi EOG6R5C6X.

    Enzyme and pathway databases

    BioCyci CBUR227377:GJ7S-228-MONOMER.
    CBUR227377:GJ7S-242-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00118_B. EF_Tu_B.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RSA 493 / Nine Mile phase I.
    2. "Differential expression of translational elements by life cycle variants of Coxiella burnetii."
      Seshadri R., Hendrix L.R., Samuel J.E.
      Infect. Immun. 67:6026-6033(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-358, DEVELOPMENTAL STAGE.
      Strain: RSA 493 / Nine Mile phase I.
    3. "Proteome and antigen profiling of Coxiella burnetii developmental forms."
      Coleman S.A., Fischer E.R., Cockrell D.C., Voth D.E., Howe D., Mead D.J., Samuel J.E., Heinzen R.A.
      Infect. Immun. 75:290-298(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, DEVELOPMENTAL STAGE.
      Strain: Nine Mile Crazy / RSA 514.

    Entry informationi

    Entry nameiEFTU_COXBU
    AccessioniPrimary (citable) accession number: Q83ES6
    Secondary accession number(s): B5QS64, Q83ET7, Q9X6G1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Coxiella burnetii
      Coxiella burnetii (strain RSA 493): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3