Reviewed, UniProtKB/Swiss-Prot Q83EQ2 (RPOA_COXBU)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase subunit alpha
      Short name=RNAP subunit alpha
    EC=2.7.7.6
Alternative name(s):
    Transcriptase subunit alpha
    RNA polymerase subunit alpha

Gene names

Name:	rpoA
Ordered Locus Names:	CBU_0263

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_00059
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00059
Subunit structure	Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Domain	The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements By similarity.
Sequence similarities	Belongs to the RNA polymerase alpha chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	DNA-directed RNA polymerase subunit alpha HAMAP MF_00059		PRO_0000175300
Regions
	Region	1 – 233	233	Alpha N-terminal domain (alpha-NTD) By similarity
	Region	247 – 327	81	Alpha C-terminal domain (alpha-CTD) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83EQ2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: AC77E3F3C3F664AC	FASTA	327	35,556
	10 20 30 40 50 60 MQNVLKSFLT PKNIQVQTIS PCHFRILLEP LERGFGHTLG NALRRILLSS MPGAAIVQAE 70 80 90 100 110 120 IDGVLHEYSS IEGVREDVVD VLLNLKGVAI KLEGREDAKL TLHKKGAGTV TAGDIQTESG 130 140 150 160 170 180 VKIVNPDHVI AHITKDGEIN MTLKAAMGRG YEPSSARSTG DQSRSVGLLL LDASYSPIRR 190 200 210 220 230 240 VTYSVENARV EKRTDLDKLI IDLETDGTLD PEEAIRFAAA VLQHQLAAFV DLKQESDRDG 250 260 270 280 290 300 GGKEGKVNPL LLRPVEDLEL TVRAANCLKA ESINYIGDLV QCTENDLLKT PNLGKKSLLE 310 320 IKSVLAQKGL SLGMDLKGWP PADLTDQ

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO89821.1.
RefSeq	NP_819307.1.
3D structure databases
SMR	Q83EQ2. Positions 248-327.
ModBase	Search...
Genome annotation databases
GeneID	1208144.
GenomeReviews	Gene locus CBU_0263 in contig AE016828_GR.
KEGG	cbu:CBU_0263.
NMPDR	fig|227377.1.peg.251.
TIGR	CBU_0263.
Phylogenomic databases
HOGENOM	HBG430844.
OMA	FGTTLGN.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0263-MON.
BRENDA	2.7.7.6. 256353.
Family and domain databases
HAMAP	MF_00059. [Tree]
InterPro	IPR011261. DNA-dir_RNA_pol_dimersation. IPR011262. DNA-dir_RNA_pol_insert. IPR009025. DNA-dir_RNA_pol_RBP11-like. IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3. IPR011260. RNAP_asu_C. IPR011773. RpoA. [Graphical view]
Gene3D	G3DSA:2.170.120.12. RNAP_insert. 1 hit.
Pfam	PF01000. RNA_pol_A_bac. 1 hit. PF03118. RNA_pol_A_CTD. 1 hit. PF01193. RNA_pol_L. 1 hit. [Graphical view]
ProDom	PD001179. RNAP_asu_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00662. RPOLD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOA_COXBU

Accession

Primary (citable) accession number: Q83EQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	June 1, 2003
Last modified:	December 15, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)