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Q83EH9

- PFKA_COXBU

UniProt

Q83EH9 - PFKA_COXBU

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141ATP; via amide nitrogenUniRule annotation
    Metal bindingi106 – 1061Magnesium; catalyticUniRule annotation
    Active sitei131 – 1311Proton acceptorUniRule annotation
    Binding sitei158 – 1581Allosteric activator ADPUniRule annotation
    Binding sitei166 – 1661Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei215 – 2151Allosteric activator ADPUniRule annotation
    Binding sitei225 – 2251SubstrateUniRule annotation
    Binding sitei248 – 2481Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 762ATPUniRule annotation
    Nucleotide bindingi105 – 1084ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCBUR227377:GJ7S-346-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:CBU_0341
    OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
    Taxonomic identifieri227377 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
    ProteomesiUP000002671: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326ATP-dependent 6-phosphofructokinasePRO_1000072055Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi227377.CBU_0341.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83EH9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni24 – 285Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni129 – 1313Substrate bindingUniRule annotation
    Regioni173 – 1753Substrate bindingUniRule annotation
    Regioni189 – 1913Allosteric activator ADP bindingUniRule annotation
    Regioni216 – 2183Allosteric activator ADP bindingUniRule annotation
    Regioni254 – 2574Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248870.
    KOiK00850.
    OMAiGFGGRCV.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q83EH9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEIKKLIVL TSGGDAPGMN AAIRAVVRTA LHYQFEVYGA TAGFAGLVNG    50
    QVVPLNSRAV ANCIQRGGTI LKTGRFENFR FKAVRDKARE FLKKLQIDAM 100
    IVLGGNGSFA GASKLYQEGG PQMIGIPCTI DNDIQGTDYC IGFDTACNTA 150
    LQAIDKIRDT AFSHERNFLI EVMGRSSGFI AVNVGIAGGA EIIALPEFHV 200
    DIDTLTQKIK KQHGKKSASI IVAAEANQPG HSFEVAKQIK EKTGIEYRVC 250
    VLGHTQRGGT PTVKDRVLAS LMGAQAIEAL KKGLTEKMIA YQNGKIAVAP 300
    LPDPDNGTRY FADEALLRVN NIICAM 326
    Length:326
    Mass (Da):35,012
    Last modified:June 1, 2003 - v1
    Checksum:iDC1EFC0038E87573
    GO

    Sequence cautioni

    The sequence AAO89897.2 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO89897.2. Different initiation.
    RefSeqiNP_819383.2. NC_002971.3.

    Genome annotation databases

    EnsemblBacteriaiAAO89897; AAO89897; CBU_0341.
    GeneIDi1208223.
    KEGGicbu:CBU_0341.
    PATRICi17929375. VBICoxBur82552_0335.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO89897.2 . Different initiation.
    RefSeqi NP_819383.2. NC_002971.3.

    3D structure databases

    ProteinModelPortali Q83EH9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 227377.CBU_0341.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO89897 ; AAO89897 ; CBU_0341 .
    GeneIDi 1208223.
    KEGGi cbu:CBU_0341.
    PATRICi 17929375. VBICoxBur82552_0335.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248870.
    KOi K00850.
    OMAi GFGGRCV.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci CBUR227377:GJ7S-346-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RSA 493 / Nine Mile phase I.

    Entry informationi

    Entry nameiPFKA_COXBU
    AccessioniPrimary (citable) accession number: Q83EH9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Coxiella burnetii
      Coxiella burnetii (strain RSA 493): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3