Q83EH9 (K6PF_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	CBU_0341

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.
Sequence caution	The sequence AAO89897.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_1000072055
Regions
	Nucleotide binding	24 – 28	5	ATP By similarity
	Nucleotide binding	158 – 162	5	ATP By similarity
	Nucleotide binding	175 – 191	17	ATP By similarity
Sites
	Active site	131	1	Proton acceptor By similarity
	Metal binding	189	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	191	1	Magnesium By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity
	Binding site	257	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83EH9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: DC1EFC0038E87573	FASTA	326	35,012
	10 20 30 40 50 60 MTEIKKLIVL TSGGDAPGMN AAIRAVVRTA LHYQFEVYGA TAGFAGLVNG QVVPLNSRAV 70 80 90 100 110 120 ANCIQRGGTI LKTGRFENFR FKAVRDKARE FLKKLQIDAM IVLGGNGSFA GASKLYQEGG 130 140 150 160 170 180 PQMIGIPCTI DNDIQGTDYC IGFDTACNTA LQAIDKIRDT AFSHERNFLI EVMGRSSGFI 190 200 210 220 230 240 AVNVGIAGGA EIIALPEFHV DIDTLTQKIK KQHGKKSASI IVAAEANQPG HSFEVAKQIK 250 260 270 280 290 300 EKTGIEYRVC VLGHTQRGGT PTVKDRVLAS LMGAQAIEAL KKGLTEKMIA YQNGKIAVAP 310 320 LPDPDNGTRY FADEALLRVN NIICAM

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO89897.2. Different initiation.
RefSeq	NP_819383.2. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83EH9.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_0341.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO89897; AAO89897; CBU_0341.
GeneID	1208223.
KEGG	cbu:CBU_0341.
PATRIC	17929375. VBICoxBur82552_0335.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248870.
KO	K00850.
OMA	EFKTVEG.
ProtClustDB	CLSK914001.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-346-MONOMER.
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_COXBU

Accession

Primary (citable) accession number: Q83EH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 1, 2003
Last modified:	May 29, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program