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Q83EH9

- PFKA_COXBU

UniProt

Q83EH9 - PFKA_COXBU

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, CBU_0341
Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141ATP; via amide nitrogen By similarity
Metal bindingi106 – 1061Magnesium; catalytic By similarity
Active sitei131 – 1311Proton acceptor By similarity
Binding sitei158 – 1581Allosteric activator ADP By similarity
Binding sitei166 – 1661Substrate; shared with dimeric partner By similarity
Binding sitei215 – 2151Allosteric activator ADP By similarity
Binding sitei225 – 2251Substrate By similarity
Binding sitei248 – 2481Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 762ATP By similarity
Nucleotide bindingi105 – 1084ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBUR227377:GJ7S-346-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:CBU_0341
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000002671: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326ATP-dependent 6-phosphofructokinaseUniRule annotationPRO_1000072055Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_0341.

Structurei

3D structure databases

ProteinModelPortaliQ83EH9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 285Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni129 – 1313Substrate binding By similarity
Regioni173 – 1753Substrate binding By similarity
Regioni189 – 1913Allosteric activator ADP binding By similarity
Regioni216 – 2183Allosteric activator ADP binding By similarity
Regioni254 – 2574Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiGFGGRCV.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83EH9-1 [UniParc]FASTAAdd to Basket

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MTEIKKLIVL TSGGDAPGMN AAIRAVVRTA LHYQFEVYGA TAGFAGLVNG    50
QVVPLNSRAV ANCIQRGGTI LKTGRFENFR FKAVRDKARE FLKKLQIDAM 100
IVLGGNGSFA GASKLYQEGG PQMIGIPCTI DNDIQGTDYC IGFDTACNTA 150
LQAIDKIRDT AFSHERNFLI EVMGRSSGFI AVNVGIAGGA EIIALPEFHV 200
DIDTLTQKIK KQHGKKSASI IVAAEANQPG HSFEVAKQIK EKTGIEYRVC 250
VLGHTQRGGT PTVKDRVLAS LMGAQAIEAL KKGLTEKMIA YQNGKIAVAP 300
LPDPDNGTRY FADEALLRVN NIICAM 326
Length:326
Mass (Da):35,012
Last modified:June 1, 2003 - v1
Checksum:iDC1EFC0038E87573
GO

Sequence cautioni

The sequence AAO89897.2 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016828 Genomic DNA. Translation: AAO89897.2. Different initiation.
RefSeqiNP_819383.2. NC_002971.3.

Genome annotation databases

EnsemblBacteriaiAAO89897; AAO89897; CBU_0341.
GeneIDi1208223.
KEGGicbu:CBU_0341.
PATRICi17929375. VBICoxBur82552_0335.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016828 Genomic DNA. Translation: AAO89897.2 . Different initiation.
RefSeqi NP_819383.2. NC_002971.3.

3D structure databases

ProteinModelPortali Q83EH9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 227377.CBU_0341.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO89897 ; AAO89897 ; CBU_0341 .
GeneIDi 1208223.
KEGGi cbu:CBU_0341.
PATRICi 17929375. VBICoxBur82552_0335.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248870.
KOi K00850.
OMAi GFGGRCV.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci CBUR227377:GJ7S-346-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RSA 493 / Nine Mile phase I.

Entry informationi

Entry nameiPFKA_COXBU
AccessioniPrimary (citable) accession number: Q83EH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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