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Q83EH9 (PFKA_COXBU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:CBU_0341
OrganismCoxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]
Taxonomic identifier227377 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_00339

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.

Sequence caution

The sequence AAO89897.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termAllosteric enzyme
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_00339
PRO_1000072055

Regions

Nucleotide binding75 – 762ATP By similarity
Nucleotide binding105 – 1084ATP By similarity
Region24 – 285Allosteric activator ADP binding; shared with dimeric partner By similarity
Region129 – 1313Substrate binding By similarity
Region173 – 1753Substrate binding By similarity
Region189 – 1913Allosteric activator ADP binding By similarity
Region216 – 2183Allosteric activator ADP binding By similarity
Region254 – 2574Substrate binding By similarity

Sites

Active site1311Proton acceptor By similarity
Metal binding1061Magnesium; catalytic By similarity
Binding site141ATP; via amide nitrogen By similarity
Binding site1581Allosteric activator ADP By similarity
Binding site1661Substrate; shared with dimeric partner By similarity
Binding site2151Allosteric activator ADP By similarity
Binding site2251Substrate By similarity
Binding site2481Substrate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83EH9 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: DC1EFC0038E87573

FASTA32635,012
        10         20         30         40         50         60 
MTEIKKLIVL TSGGDAPGMN AAIRAVVRTA LHYQFEVYGA TAGFAGLVNG QVVPLNSRAV 

        70         80         90        100        110        120 
ANCIQRGGTI LKTGRFENFR FKAVRDKARE FLKKLQIDAM IVLGGNGSFA GASKLYQEGG 

       130        140        150        160        170        180 
PQMIGIPCTI DNDIQGTDYC IGFDTACNTA LQAIDKIRDT AFSHERNFLI EVMGRSSGFI 

       190        200        210        220        230        240 
AVNVGIAGGA EIIALPEFHV DIDTLTQKIK KQHGKKSASI IVAAEANQPG HSFEVAKQIK 

       250        260        270        280        290        300 
EKTGIEYRVC VLGHTQRGGT PTVKDRVLAS LMGAQAIEAL KKGLTEKMIA YQNGKIAVAP 

       310        320 
LPDPDNGTRY FADEALLRVN NIICAM 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016828 Genomic DNA. Translation: AAO89897.2. Different initiation.
RefSeqNP_819383.2. NC_002971.3.

3D structure databases

ProteinModelPortalQ83EH9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227377.CBU_0341.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO89897; AAO89897; CBU_0341.
GeneID1208223.
KEGGcbu:CBU_0341.
PATRIC17929375. VBICoxBur82552_0335.

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248870.
KOK00850.
OMAGFGGRCV.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycCBUR227377:GJ7S-346-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase_I_B1.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_COXBU
AccessionPrimary (citable) accession number: Q83EH9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Coxiella burnetii

Coxiella burnetii (strain RSA 493): entries and gene names