Q83EA3 (PANC_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate synthetase
Short name=PS
EC=6.3.2.1

Alternative name(s):
Pantoate--beta-alanine ligase
Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	CBU_0423

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP-Rule MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP-Rule MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP-Rule MF_00158
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP-Rule MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Pantothenate synthetase HAMAP-Rule MF_00158		PRO_0000305432
Regions
	Nucleotide binding	29 – 36	8	ATP By similarity
	Nucleotide binding	145 – 148	4	ATP By similarity
	Nucleotide binding	182 – 185	4	ATP By similarity
Sites
	Active site	36	1	Proton donor By similarity
	Binding site	60	1	Beta-alanine By similarity
	Binding site	60	1	Pantoate By similarity
	Binding site	151	1	Pantoate By similarity
	Binding site	174	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83EA3 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2A8DD0535687A595	FASTA	257	29,479
	10 20 30 40 50 60 MTKVIEALSD WQSIRKTIND LSVGFVPTMG NLHAGHLSLL ERSKCENTIT VLSLFINPTQ 70 80 90 100 110 120 FNDKNDFKNY PRTLAQDIAM AEENGIDYVL APTDDALYPD QYAYKITNST INNQEAEFRP 130 140 150 160 170 180 RHFDGVLTVV MKLLLLVKPT RAYFGEKDYQ QLQLVKGLAE AFFLDTEIIG CKIVRNEFGL 190 200 210 220 230 240 PLSSRNRRLT EDQYQLAQRF SEIFHSDLSC DEIKNALIQE GIIVDYIEDY NERRFAAVHV 250 GDIRLIDNIP FAKDKKC

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO89975.1.
RefSeq	NP_819461.1. NC_002971.3.
3D structure databases
HSSP	HSSP built from PDB template 1IHO based on UniProtKB P31663.
ProteinModelPortal	Q83EA3.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_0423.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO89975; AAO89975; CBU_0423.
GeneID	1208307.
KEGG	cbu:CBU_0423.
PATRIC	17929533. VBICoxBur82552_0412.
Phylogenomic databases
eggNOG	COG0414.
HOGENOM	HOG000175517.
KO	K01918.
OMA	KENHYTV.
ProtClustDB	CLSK914057.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-421-MONOMER.
UniPathway	UPA00028; UER00005.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00158. PanC.
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR21299:SF1. PTHR21299:SF1. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_COXBU

Accession

Primary (citable) accession number: Q83EA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program