Q83E97 (SYK_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysine--tRNA ligase
EC=6.1.1.6

Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS

Gene names

Name:	lysS
Ordered Locus Names:	CBU_0430

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Lysine--tRNA ligase HAMAP-Rule MF_00252		PRO_0000152620
Sites
	Metal binding	409	1	Magnesium 1 By similarity
	Metal binding	416	1	Magnesium 1 By similarity
	Metal binding	416	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83E97 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8092BC68989DAC00	FASTA	498	57,739
	10 20 30 40 50 60 MELKDQIKEE NEQIAQRKLK LKKRREEGQA YPNDFKRDSL AADLHAVYDQ FDSGALTAKA 70 80 90 100 110 120 IRVKMAGRMM TRRIMGKASF AHIQDMKGRM QIYVTRDSLP QGVYSDFKSW DLGDIVGIEG 130 140 150 160 170 180 ELFKTKTEEL SVKVDQIRLL TKALRPMPDK FHGLHDQEQR FRQRYLDLIV NESSRHLFQT 190 200 210 220 230 240 RSQVIAQIRR FLDDRGYIEV ETPMMHPLPG GAAARPFETH HNAMNMDLFL RIAPELYLKR 250 260 270 280 290 300 LVVGGFEKVY EINRNFRNEG ISTRHNPEFT MLEFYQAYAT YEDMMMLTES MIRHLAEKIF 310 320 330 340 350 360 GVMEIKYQGV RIDLNKPFPR LSLRDAILQF NPGITPDQID HLETARELAH KYEIATPAHY 370 380 390 400 410 420 GLGKIQTELF EKLVEEKLQQ PIFITHFPKE VSPLSRANEE NDFITDRFEF YVGGREIANG 430 440 450 460 470 480 FSELNDPEDQ AARFREQLKA RNAGDLEAMS FDEDYITALE YGLPPTAGEG IGIDRLVMLF 490 TDNASIRDVI LFPLLRSK

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO89981.1.
RefSeq	NP_819467.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83E97.
SMR	Q83E97. Positions 7-497.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_0430.
Proteomic databases
PRIDE	Q83E97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO89981; AAO89981; CBU_0430.
GeneID	1208314.
KEGG	cbu:CBU_0430.
PATRIC	17929547. VBICoxBur82552_0419.
Phylogenomic databases
eggNOG	COG1190.
HOGENOM	HOG000236578.
KO	K04567.
OMA	EHKLEQP.
ProtClustDB	PRK00484.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-428-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_00252. Lys_tRNA_synth_class2.
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR002313. Lys-tRNA-ligase_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. PTHR22594. 1 hit. PTHR22594:SF4. PTHR22594:SF4. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00499. lysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK_COXBU

Accession

Primary (citable) accession number: Q83E97

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program