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Protein

Lysine--tRNA ligase

Gene

lysS

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 3 Mg2+ ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi409 – 4091Magnesium 1UniRule annotation
Metal bindingi416 – 4161Magnesium 1UniRule annotation
Metal bindingi416 – 4161Magnesium 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBUR227377:GJ7S-428-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligaseUniRule annotation (EC:6.1.1.6UniRule annotation)
Alternative name(s):
Lysyl-tRNA synthetaseUniRule annotation
Short name:
LysRSUniRule annotation
Gene namesi
Name:lysSUniRule annotation
Ordered Locus Names:CBU_0430
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Lysine--tRNA ligasePRO_0000152620Add
BLAST

Proteomic databases

PRIDEiQ83E97.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_0430.

Structurei

3D structure databases

ProteinModelPortaliQ83E97.
SMRiQ83E97. Positions 7-497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1190.
HOGENOMiHOG000236578.
KOiK04567.
OMAiHHNTLDI.
OrthoDBiEOG69PQ2M.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83E97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELKDQIKEE NEQIAQRKLK LKKRREEGQA YPNDFKRDSL AADLHAVYDQ
60 70 80 90 100
FDSGALTAKA IRVKMAGRMM TRRIMGKASF AHIQDMKGRM QIYVTRDSLP
110 120 130 140 150
QGVYSDFKSW DLGDIVGIEG ELFKTKTEEL SVKVDQIRLL TKALRPMPDK
160 170 180 190 200
FHGLHDQEQR FRQRYLDLIV NESSRHLFQT RSQVIAQIRR FLDDRGYIEV
210 220 230 240 250
ETPMMHPLPG GAAARPFETH HNAMNMDLFL RIAPELYLKR LVVGGFEKVY
260 270 280 290 300
EINRNFRNEG ISTRHNPEFT MLEFYQAYAT YEDMMMLTES MIRHLAEKIF
310 320 330 340 350
GVMEIKYQGV RIDLNKPFPR LSLRDAILQF NPGITPDQID HLETARELAH
360 370 380 390 400
KYEIATPAHY GLGKIQTELF EKLVEEKLQQ PIFITHFPKE VSPLSRANEE
410 420 430 440 450
NDFITDRFEF YVGGREIANG FSELNDPEDQ AARFREQLKA RNAGDLEAMS
460 470 480 490
FDEDYITALE YGLPPTAGEG IGIDRLVMLF TDNASIRDVI LFPLLRSK
Length:498
Mass (Da):57,739
Last modified:June 1, 2003 - v1
Checksum:i8092BC68989DAC00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO89981.1.
RefSeqiNP_819467.1. NC_002971.3.
WP_005771651.1. NZ_CCYB01000054.1.

Genome annotation databases

EnsemblBacteriaiAAO89981; AAO89981; CBU_0430.
GeneIDi1208314.
KEGGicbu:CBU_0430.
PATRICi17929547. VBICoxBur82552_0419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO89981.1.
RefSeqiNP_819467.1. NC_002971.3.
WP_005771651.1. NZ_CCYB01000054.1.

3D structure databases

ProteinModelPortaliQ83E97.
SMRiQ83E97. Positions 7-497.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227377.CBU_0430.

Proteomic databases

PRIDEiQ83E97.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO89981; AAO89981; CBU_0430.
GeneIDi1208314.
KEGGicbu:CBU_0430.
PATRICi17929547. VBICoxBur82552_0419.

Phylogenomic databases

eggNOGiCOG1190.
HOGENOMiHOG000236578.
KOiK04567.
OMAiHHNTLDI.
OrthoDBiEOG69PQ2M.

Enzyme and pathway databases

BioCyciCBUR227377:GJ7S-428-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00252. Lys_tRNA_synth_class2.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR00982. TRNASYNTHLYS.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00499. lysS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RSA 493 / Nine Mile phase I.

Entry informationi

Entry nameiSYK_COXBU
AccessioniPrimary (citable) accession number: Q83E97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: July 22, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.