Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-phosphoshikimate 1-carboxyvinyltransferase

Gene

aroA

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (CBU_0982)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26Shikimate-3-phosphateUniRule annotation1 Publication1
Binding sitei123PhosphoenolpyruvateUniRule annotation1 Publication1
Active sitei315Proton acceptorUniRule annotation1 Publication1
Binding sitei342Shikimate-3-phosphateUniRule annotation1 Publication1
Active sitei343Proton donorUniRule annotation1
Binding sitei346PhosphoenolpyruvateUniRule annotation1 Publication1
Binding sitei387PhosphoenolpyruvateUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation
Short name:
EPSP synthaseUniRule annotation
Short name:
EPSPSUniRule annotation
Gene namesi
Name:aroAUniRule annotation
Ordered Locus Names:CBU_0526
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000717371 – 4383-phosphoshikimate 1-carboxyvinyltransferaseAdd BLAST438

Proteomic databases

PRIDEiQ83E11.

Interactioni

Subunit structurei

Homodimer or homotetramer.3 Publications

Protein-protein interaction databases

STRINGi227377.CBU_0526.

Structurei

Secondary structure

1438
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 5Combined sources5
Beta strandi12 – 16Combined sources5
Helixi21 – 33Combined sources13
Beta strandi34 – 42Combined sources9
Helixi47 – 58Combined sources12
Beta strandi62 – 66Combined sources5
Helixi67 – 69Combined sources3
Beta strandi71 – 75Combined sources5
Helixi95 – 105Combined sources11
Beta strandi108 – 115Combined sources8
Helixi120 – 122Combined sources3
Helixi126 – 134Combined sources9
Beta strandi138 – 142Combined sources5
Beta strandi145 – 151Combined sources7
Beta strandi160 – 162Combined sources3
Beta strandi164 – 166Combined sources3
Helixi168 – 178Combined sources11
Beta strandi181 – 188Combined sources8
Helixi196 – 203Combined sources8
Beta strandi215 – 218Combined sources4
Helixi234 – 246Combined sources13
Beta strandi251 – 258Combined sources8
Helixi261 – 263Combined sources3
Helixi265 – 273Combined sources9
Beta strandi276 – 285Combined sources10
Beta strandi288 – 296Combined sources9
Helixi307 – 309Combined sources3
Helixi310 – 313Combined sources4
Helixi314 – 316Combined sources3
Helixi317 – 325Combined sources9
Beta strandi327 – 333Combined sources7
Helixi337 – 341Combined sources5
Beta strandi342 – 344Combined sources3
Helixi346 – 356Combined sources11
Beta strandi360 – 364Combined sources5
Beta strandi367 – 371Combined sources5
Beta strandi378 – 381Combined sources4
Helixi386 – 398Combined sources13
Beta strandi399 – 401Combined sources3
Beta strandi403 – 406Combined sources4
Helixi409 – 413Combined sources5
Helixi418 – 425Combined sources8
Beta strandi429 – 433Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ROIX-ray2.20A/B1-438[»]
3SLHX-ray1.70A/B/C/D1-438[»]
3TR1X-ray2.00A1-438[»]
4EGRX-ray2.50A/B/C/D/E/F1-438[»]
4GFPX-ray2.70A1-438[»]
4ZNDX-ray2.55A1-438[»]
ProteinModelPortaliQ83E11.
SMRiQ83E11.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 22Shikimate-3-phosphate bindingUniRule annotation1 Publication2
Regioni93 – 96PhosphoenolpyruvateUniRule annotation2 Publications4

Sequence similaritiesi

Belongs to the EPSP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CMY. Bacteria.
COG0128. LUCA.
HOGENOMiHOG000247371.
KOiK00800.
OMAiMAFADCG.

Family and domain databases

CDDicd01556. EPSP_synthase. 1 hit.
Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth. 1 hit.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83E11-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYQTIPSQG LSGEICVPGD KSISHRAVLL AAIAEGQTQV DGFLMGADNL
60 70 80 90 100
AMVSALQQMG ASIQVIEDEN ILVVEGVGMT GLQAPPEALD CGNSGTAIRL
110 120 130 140 150
LSGLLAGQPF NTVLTGDSSL QRRPMKRIID PLTLMGAKID STGNVPPLKI
160 170 180 190 200
YGNPRLTGIH YQLPMASAQV KSCLLLAGLY ARGKTCITEP APSRDHTERL
210 220 230 240 250
LKHFHYTLQK DKQSICVSGG GKLKANDISI PGDISSAAFF IVAATITPGS
260 270 280 290 300
AIRLCRVGVN PTRLGVINLL KMMGADIEVT HYTEKNEEPT ADITVRHARL
310 320 330 340 350
KGIDIPPDQV PLTIDEFPVL LIAAAVAQGK TVLRDAAELR VKETDRIAAM
360 370 380 390 400
VDGLQKLGIA AESLPDGVII QGGTLEGGEV NSYDDHRIAM AFAVAGTLAK
410 420 430
GPVRIRNCDN VKTSFPNFVE LANEVGMNVK GVRGRGGF
Length:438
Mass (Da):46,432
Last modified:June 1, 2003 - v1
Checksum:iEF1C0FEEEBCD4D54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90072.1.
RefSeqiNP_819558.1. NC_002971.3.
WP_010957637.1. NC_002971.3.

Genome annotation databases

EnsemblBacteriaiAAO90072; AAO90072; CBU_0526.
GeneIDi1208411.
KEGGicbu:CBU_0526.
PATRICi17929753. VBICoxBur82552_0521.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90072.1.
RefSeqiNP_819558.1. NC_002971.3.
WP_010957637.1. NC_002971.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ROIX-ray2.20A/B1-438[»]
3SLHX-ray1.70A/B/C/D1-438[»]
3TR1X-ray2.00A1-438[»]
4EGRX-ray2.50A/B/C/D/E/F1-438[»]
4GFPX-ray2.70A1-438[»]
4ZNDX-ray2.55A1-438[»]
ProteinModelPortaliQ83E11.
SMRiQ83E11.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227377.CBU_0526.

Proteomic databases

PRIDEiQ83E11.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO90072; AAO90072; CBU_0526.
GeneIDi1208411.
KEGGicbu:CBU_0526.
PATRICi17929753. VBICoxBur82552_0521.

Phylogenomic databases

eggNOGiENOG4105CMY. Bacteria.
COG0128. LUCA.
HOGENOMiHOG000247371.
KOiK00800.
OMAiMAFADCG.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089.

Family and domain databases

CDDicd01556. EPSP_synthase. 1 hit.
Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth. 1 hit.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROA_COXBU
AccessioniPrimary (citable) accession number: Q83E11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.