Q83DX3 (MIAB_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
EC=2.-.-.-

Alternative name(s):
tRNA-i(6)A37 methylthiotransferase

Gene names

Name:	miaB
Ordered Locus Names:	CBU_0569

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subunit structure	Monomer By similarity. HAMAP-Rule MF_01864
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01864.
Sequence similarities	Belongs to the methylthiotransferase family. MiaB subfamily. Contains 1 MTTase N-terminal domain. Contains 1 TRAM domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB HAMAP-Rule MF_01864		PRO_0000374244
Regions
	Domain	2 – 119	118	MTTase N-terminal
	Domain	377 – 439	63	TRAM
Sites
	Metal binding	11	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	48	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	82	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	156	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	160	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	163	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83DX3 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A506BEB9F2EA897D	FASTA	439	49,148
	10 20 30 40 50 60 MKKLYLKTHG CQMNEYDSAK MADVLKFSHG LELTEDPAVA DVFLLNTCSV REKAQTKVFS 70 80 90 100 110 120 ELGRWRPFKE KRPHVVIGVG GCVASQEGET ILKQAPFVDI VFGPQTLHRL PDLLDSVIQK 130 140 150 160 170 180 RKSVVDITFP EIEKFDRLPQ PRAEGPSAFV SIMEGCSKYC TFCVVPYTRG EEISRPFDDV 190 200 210 220 230 240 IAEVASLCEQ GVREITLLGQ NVNDYRGLMH DGQVADLALL IHYLAAMDNI ERIRFTTSHP 250 260 270 280 290 300 SAFSENLIDA YAEEPKLANH LHLPVQSGSD RILAAMKRNY TVLEYKSKIR KLRAVRPDIS 310 320 330 340 350 360 LSSDFIIGFP GETDADFEAT MNLIHDMGFD HSFSFIYSPR PGTPAAQLPD DVPMAVKKER 370 380 390 400 410 420 LAILQNRINA KAAEISQSMV GTQQRILVTG PSKKYPDQLS GRTENNRVVN FNGDTPLIGQ 430 MVTIKIKEAR PYSLWGEIC

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO90113.1.
RefSeq	NP_819599.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83DX3.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_0569.
Proteomic databases
PRIDE	Q83DX3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO90113; AAO90113; CBU_0569.
GeneID	1208454.
KEGG	cbu:CBU_0569.
PATRIC	17929837. VBICoxBur82552_0563.
Phylogenomic databases
eggNOG	COG0621.
HOGENOM	HOG000224767.
KO	K06168.
OMA	GIDRIRY.
ProtClustDB	PRK14325.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-573-MONOMER.
Family and domain databases
Gene3D	3.80.30.20. 1 hit.
HAMAP	MF_01864. tRNA_metthiotr_MiaB.
InterPro	IPR006638. Elp3/MiaB/NifB. IPR023970. MeThioTfrase/rSAM. IPR005839. Methylthiotransferase. IPR020612. Methylthiotransferase_CS. IPR013848. Methylthiotransferase_N. IPR006463. MiaB_methiolase. IPR007197. rSAM. IPR023404. rSAM_horseshoe. IPR002792. TRAM_dom. [Graphical view]
PANTHER	PTHR11918. PTHR11918. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF01938. TRAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01574. miaB-methiolase. 1 hit. TIGR00089. TIGR00089. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. PS50926. TRAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIAB_COXBU

Accession

Primary (citable) accession number: Q83DX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program