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Q83DX3

- MIAB_COXBU

UniProt

Q83DX3 - MIAB_COXBU

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Protein
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Gene
miaB, CBU_0569
Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi11 – 111Iron-sulfur (4Fe-4S) By similarity
Metal bindingi48 – 481Iron-sulfur (4Fe-4S) By similarity
Metal bindingi82 – 821Iron-sulfur (4Fe-4S) By similarity
Metal bindingi156 – 1561Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi160 – 1601Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi163 – 1631Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. transferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBUR227377:GJ7S-573-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (EC:2.8.4.3)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
tRNA-i(6)A37 methylthiotransferase
Gene namesi
Name:miaB
Ordered Locus Names:CBU_0569
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000002671: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation
PRO_0000374244Add
BLAST

Proteomic databases

PRIDEiQ83DX3.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_0569.

Structurei

3D structure databases

ProteinModelPortaliQ83DX3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 119118MTTase N-terminal
Add
BLAST
Domaini377 – 43963TRAM
Add
BLAST

Sequence similaritiesi

Contains 1 TRAM domain.

Phylogenomic databases

eggNOGiCOG0621.
HOGENOMiHOG000224767.
KOiK06168.
OMAiIEVYKDT.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83DX3-1 [UniParc]FASTAAdd to Basket

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MKKLYLKTHG CQMNEYDSAK MADVLKFSHG LELTEDPAVA DVFLLNTCSV    50
REKAQTKVFS ELGRWRPFKE KRPHVVIGVG GCVASQEGET ILKQAPFVDI 100
VFGPQTLHRL PDLLDSVIQK RKSVVDITFP EIEKFDRLPQ PRAEGPSAFV 150
SIMEGCSKYC TFCVVPYTRG EEISRPFDDV IAEVASLCEQ GVREITLLGQ 200
NVNDYRGLMH DGQVADLALL IHYLAAMDNI ERIRFTTSHP SAFSENLIDA 250
YAEEPKLANH LHLPVQSGSD RILAAMKRNY TVLEYKSKIR KLRAVRPDIS 300
LSSDFIIGFP GETDADFEAT MNLIHDMGFD HSFSFIYSPR PGTPAAQLPD 350
DVPMAVKKER LAILQNRINA KAAEISQSMV GTQQRILVTG PSKKYPDQLS 400
GRTENNRVVN FNGDTPLIGQ MVTIKIKEAR PYSLWGEIC 439
Length:439
Mass (Da):49,148
Last modified:June 1, 2003 - v1
Checksum:iA506BEB9F2EA897D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016828 Genomic DNA. Translation: AAO90113.1.
RefSeqiNP_819599.1. NC_002971.3.

Genome annotation databases

EnsemblBacteriaiAAO90113; AAO90113; CBU_0569.
GeneIDi1208454.
KEGGicbu:CBU_0569.
PATRICi17929837. VBICoxBur82552_0563.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016828 Genomic DNA. Translation: AAO90113.1 .
RefSeqi NP_819599.1. NC_002971.3.

3D structure databases

ProteinModelPortali Q83DX3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 227377.CBU_0569.

Proteomic databases

PRIDEi Q83DX3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO90113 ; AAO90113 ; CBU_0569 .
GeneIDi 1208454.
KEGGi cbu:CBU_0569.
PATRICi 17929837. VBICoxBur82552_0563.

Phylogenomic databases

eggNOGi COG0621.
HOGENOMi HOG000224767.
KOi K06168.
OMAi IEVYKDT.
OrthoDBi EOG6P5ZD8.

Enzyme and pathway databases

BioCyci CBUR227377:GJ7S-573-MONOMER.

Family and domain databases

Gene3Di 3.80.30.20. 1 hit.
HAMAPi MF_01864. tRNA_metthiotr_MiaB.
InterProi IPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view ]
PANTHERi PTHR11918. PTHR11918. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
PROSITEi PS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RSA 493 / Nine Mile phase I.

Entry informationi

Entry nameiMIAB_COXBU
AccessioniPrimary (citable) accession number: Q83DX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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