Q83DS5 (LPXB_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipid-A-disaccharide synthase
EC=2.4.1.182

Gene names

Name:	lpxB
Ordered Locus Names:	CBU_0620

Organism

Coxiella burnetii

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00392
Catalytic activity	UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP MF_00392
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 5/6. HAMAP MF_00392
Sequence similarities	Belongs to the LpxB family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lipid-A-disaccharide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Lipid-A-disaccharide synthase HAMAP MF_00392		PRO_0000190163

Sequences

	Sequence		Length	Mass (Da)
	Q83DS5 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6D1A160A53A58E7F	FASTA	376	41,955
	10 20 30 40 50 60 MSNKSVLLIA GEPSGDLLGA HLAQSLKSLE PNLKLAGMGG KRMREAGVEV FINADKLAVV 70 80 90 100 110 120 GLLEILRQFR DIRHAMQTLK RYFKKTPPDL VVFIDYPGFN LHMAKQAKKA GIKVLYYVSP 130 140 150 160 170 180 QIWAWRYGRI KKIKKYVDHM AVLFDFEEKL YQKENVPVSF VGHPLANAPT PSLSRNEICK 190 200 210 220 230 240 QFNLDPDKPI VALFPGSREQ EINKLLPMMV QAGKLIQTQI PTVQFILPLA LNLALDKIRP 250 260 270 280 290 300 FLSPEIKVIQ NDISYVLAIA HAAVAASGTV TLEIALQQVP LVIIYKVAPL TFWLGKKLIR 310 320 330 340 350 360 LSFIGLCNLV SPEPVAVELL QQDATPQAIA DEVFQLLNNH NYRQSIIGKL GHLRPQLDRG 370 NAAQNVAKVV HNLIFS

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO90164.1.
RefSeq	NP_819650.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83DS5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1208505.
GenomeReviews	Gene locus CBU_0620 in contig AE016828_GR.
KEGG	cbu:CBU_0620.
NMPDR	fig|227377.1.peg.594.
PATRIC	17929925. VBICoxBur82552_0607.
TIGR	CBU_0620.
Phylogenomic databases
HOGENOM	HBG584837.
OMA	AEFAFIC.
PhylomeDB	Q83DS5.
ProtClustDB	PRK00025.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0620-MONOMER.
Family and domain databases
HAMAP	MF_00392. LpxB. [Tree]
InterPro	IPR003835. Glyco_trans_19. [Graphical view]
KO	K00748.
Pfam	PF02684. LpxB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00215. LpxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXB_COXBU

Accession

Primary (citable) accession number: Q83DS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program