Polyribonucleotide nucleotidyltransferase - Coxiella burnetii (strain RSA 493 / Nine Mile phase I)

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Q83D87 (PNP_COXBU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 63. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8

Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	CBU_0852

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella ›

Protein attributes

Sequence length	696 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP-Rule MF_01595
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01595.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: HAMAP polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 696

696

Polyribonucleotide nucleotidyltransferase HAMAP-Rule MF_01595

PRO_0000329612

Regions

Domain

556 – 615

Domain

625 – 693

S1 motif

Sequences

Sequence

Length

Mass (Da)

Tools

Q83D87 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 9E55E7C7CD00E52B
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FASTA

696

76,331

        10         20         30         40         50         60 
MNKIRKTFQY GKHEVTFETG EMARQATGAV VVRMGDTVLL VSVVAKKEAE EGRDFFPLTV 

        70         80         90        100        110        120 
NYQEKTYAAG KIPGGYFKRE GRPTEKETLT SRLIDRPLRP LFPKGFTNEV QVIATVLSVD 

       130        140        150        160        170        180 
SKVPTDIPAI LGASAAIGLS GIPFNGSLGA ARVGYRGGEY LLNPSLDELK DSALDLVVAG 

       190        200        210        220        230        240 
TRDAVLMVES EAQELPESVM LGAVLHGHQA MQVAIQAIAE FIQEAGGAKW EWEPPTVNTA 

       250        260        270        280        290        300 
LEKWVVEKSE APLKKAYQIQ EKTARQAQIQ AIRDQLLADR AAEREGEENA VNEHELAVIF 

       310        320        330        340        350        360 
HELERRIVRE QILTGQPRID GRDTKTVRPI TVKVGVLPRS HGSALFTRGE TQALVVTTLG 

       370        380        390        400        410        420 
TERDAQSIDD LDGDRQEEFI FHYNFPPFCV GEVGFMSGPK RREIGHGRLA KRAVVPVVPT 

       430        440        450        460        470        480 
LDKFPYVIRV VSEILESNGS SSMASVCGSS LALMDAGVPT KAPVAGIAMG LIKENDKYAV 

       490        500        510        520        530        540 
LSDILGDEDH LGDMDFKVAG TSNGVTALQM DIKIEGITKE IMEQALDQAK EGRLHILSIM 

       550        560        570        580        590        600 
NKVLDKPRSQ VSDLAPQYVT MKINPEKIRD VIGKGGVVIR EITEATNCAI DISDDGTIKI 

       610        620        630        640        650        660 
AAHTTEEGEA AKRRIEELTA EVELGKVYEG TVVKITDFGA FVQILPNTQG LVHISQIAQE 

       670        680        690 
RVENVRDYLE EGQVIRVKVI EIDRQGRVRL SMKQID

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References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii."
Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A.

Heidelberg J.F.
Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO90386.1.
RefSeq	NP_819872.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83D87.
SMR	Q83D87. Positions 620-694.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_0852.
Proteomic databases
PRIDE	Q83D87.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO90386; AAO90386; CBU_0852.
GeneID	1208745.
KEGG	cbu:CBU_0852.
PATRIC	17930401. VBICoxBur82552_0837.
Phylogenomic databases
eggNOG	COG1185.
HOGENOM	HOG000218326.
KO	K00962.
OMA	YTMRVVS.
ProtClustDB	PRK11824.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-847-MONOMER.
Family and domain databases
Gene3D	1.10.10.400. 1 hit. 2.40.50.140. 1 hit. 3.30.230.70. 2 hits.
HAMAP	MF_01595. PNPase.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH_dom. IPR004088. KH_dom_type_1. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR027408. PNPase/RNase_PH_dom. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR020568. Ribosomal_S5_D2-typ_fold. IPR022967. RNA-binding_domain_S1. [Graphical view]
PANTHER	PTHR11252. PTHR11252. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. SM00316. S1. 1 hit. [Graphical view]
SUPFAM	SSF46915. 3_ExoRNase. 1 hit. SSF55666. 3_ExoRNase. 2 hits. SSF50249. Nucleic_acid_OB. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMs	TIGR03591. polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_COXBU

Accession

Primary (citable) accession number: Q83D87

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	June 1, 2003
Last modified:	May 29, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Coxiella burnetii

Coxiella burnetii (strain RSA 493): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents