Q83D14 (GLPK_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Synonyms:	gplK
Ordered Locus Names:	CBU_0932

Organism

Coxiella burnetii

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.
Sequence caution	The sequence AAO90459.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Glycerol kinase HAMAP MF_00186		PRO_0000059449
Regions
	Nucleotide binding	409 – 413	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83D14 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 829C49CD01B95969	FASTA	501	55,387
	10 20 30 40 50 60 MSSFILAIDQ GTTSTRAILF NEEAKLVHYH HVEITQYFPQ GGWVEHDPEE IWDSTLLCCR 70 80 90 100 110 120 NVLEEASLRA ADIAALGISN QRETTILWDR HTGQPLYRAI GWQDRRTVNF CEQLASQAGV 130 140 150 160 170 180 LAKFVEKTGL ILDPYFSCSK IKWILDNIKG AYEKAKRGEL AFGTVDSYLL WKFTGGKCHA 190 200 210 220 230 240 TDATNASRTG LFNINQQRWD DELLTLFDIP KSLLPTVLDN CAQFGFTDLD LLGHKIPITA 250 260 270 280 290 300 MIGDQQAAAV GQACIKPGMV KSTYGTGCFM LLNTGDQIIH SRNRLLATIA YRLNDTVTYG 310 320 330 340 350 360 LEGSIFIAGA AVKWLRDPLH LIEKANDSES MASSVEDTGG VYLVPAFTGL GAPYWDPNAR 370 380 390 400 410 420 GALFGLTRNT QREHIVRAAL EAVCYQSKDL VRAILNDGAN LTTLRVDGGM AANNWLLQFL 430 440 450 460 470 480 SDILGVNVDR SRCIESSALG TAFLAGLGAG LFDSLEEMTG LWQADRHFIP QMDPKKREEL 490 500 YDGWQKAVEK TLTPAAPLLF P

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO90459.2. Different initiation.
RefSeq	NP_819945.2. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83D14.
SMR	Q83D14. Positions 4-492.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1208825.
GenomeReviews	Gene locus CBU_0932 in contig AE016828_GR.
KEGG	cbu:CBU_0932.
NMPDR	fig|227377.1.peg.889.
PATRIC	17930571. VBICoxBur82552_0922.
TIGR	CBU_0932.
Phylogenomic databases
HOGENOM	HBG511469.
OMA	CYQTRDL.
PhylomeDB	Q83D14.
ProtClustDB	CLSK914422.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0932-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_COXBU

Accession

Primary (citable) accession number: Q83D14

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program