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Protein

Dihydroorotate dehydrogenase (quinone)

Gene

pyrD

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

Catalytic activityi

(S)-dihydroorotate + a quinone = orotate + a quinol.

Cofactori

FMNNote: Binds 1 FMN per subunit.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes orotate from (S)-dihydroorotate (quinone route).
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydroorotate dehydrogenase (quinone) (pyrD)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes orotate from (S)-dihydroorotate (quinone route), the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66Substrate1
Binding sitei86FMN; via amide nitrogen1
Binding sitei139FMN1
Binding sitei172FMN1
Binding sitei172Substrate1
Active sitei175Nucleophile1
Binding sitei177Substrate1
Binding sitei217FMN1
Binding sitei245FMN; via carbonyl oxygen1
Binding sitei268FMN; via amide nitrogen1
Binding sitei297FMN; via amide nitrogen1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 66FMN5
Nucleotide bindingi318 – 319FMN2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyrimidine biosynthesis
LigandFlavoprotein, FMN

Enzyme and pathway databases

UniPathwayiUPA00070; UER00946.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroorotate dehydrogenase (quinone) (EC:1.3.5.2)
Alternative name(s):
DHOdehase
Short name:
DHOD
Short name:
DHODase
Dihydroorotate oxidase
Gene namesi
Name:pyrD
Ordered Locus Names:CBU_0971
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cell membrane ; Peripheral membrane protein

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001484351 – 347Dihydroorotate dehydrogenase (quinone)Add BLAST347

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi227377.CBU_0971.

Structurei

3D structure databases

ProteinModelPortaliQ83CY0.
SMRiQ83CY0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni111 – 115Substrate binding5
Regioni246 – 247Substrate binding2

Sequence similaritiesi

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Phylogenomic databases

eggNOGiENOG4107QYT. Bacteria.
COG0167. LUCA.
HOGENOMiHOG000225103.
KOiK00254.
OMAiLQNAMGF.

Family and domain databases

CDDicd04738. DHOD_2_like. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00225. DHO_dh_type2. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
PfamiView protein in Pfam
PF01180. DHO_dh. 1 hit.
PIRSFiPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsiTIGR01036. pyrD_sub2. 1 hit.
PROSITEiView protein in PROSITE
PS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83CY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYRYLRPWL FKLEPETAHA LTLNCLKWFY CYWLINRRLQ RFPQKPTVVF
60 70 80 90 100
GIEFPNPVGL AAGLDKNGEY MDELLGLGFG FIEVGAVTPK PQPGNSKPRI
110 120 130 140 150
FRLPQARALI NRMGFNNLGV DYLVEQLKRR KVKGIVGVNI GKNLTTPLEK
160 170 180 190 200
AHEDYQNCFE KLYSYVDYVT INISSPNTPE LRQLQSERYL ADLLTRLKED
210 220 230 240 250
QRRLEDQYHK RVPLFLKIAP DLTPEEIQTI ATLALQHRIE GIVATNTSCS
260 270 280 290 300
RQGTEKLPNA NEAGGLSGKP LFPMTLQVVK QLHSFLGDEI PIVAVGGIFS
310 320 330 340
GENAQTLINA GARLVQLYTG LIYEGPELVK NIVEFLTLSR QTREGIN
Length:347
Mass (Da):39,314
Last modified:June 1, 2003 - v1
Checksum:i116F7122D9C3704D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90493.1.
RefSeqiNP_819979.1. NC_002971.3.
WP_005768550.1. NZ_CDBG01000001.1.

Genome annotation databases

EnsemblBacteriaiAAO90493; AAO90493; CBU_0971.
GeneIDi1208866.
31483697.
KEGGicbu:CBU_0971.
PATRICifig|227377.7.peg.968.

Similar proteinsi

Entry informationi

Entry nameiPYRD_COXBU
AccessioniPrimary (citable) accession number: Q83CY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2003
Last modified: June 7, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families