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Protein

Dihydroorotate dehydrogenase (quinone)

Gene

pyrD

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.UniRule annotation

Catalytic activityi

(S)-dihydroorotate + a quinone = orotate + a quinol.UniRule annotation

Cofactori

FMNUniRule annotationNote: Binds 1 FMN per subunit.UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes orotate from (S)-dihydroorotate (quinone route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydroorotate dehydrogenase (quinone) (pyrD)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes orotate from (S)-dihydroorotate (quinone route), the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66SubstrateUniRule annotation1
Binding sitei86FMN; via amide nitrogenUniRule annotation1
Binding sitei139FMNUniRule annotation1
Binding sitei172FMNUniRule annotation1
Binding sitei172SubstrateUniRule annotation1
Active sitei175NucleophileUniRule annotation1
Binding sitei177SubstrateUniRule annotation1
Binding sitei217FMNUniRule annotation1
Binding sitei245FMN; via carbonyl oxygenUniRule annotation1
Binding sitei268FMN; via amide nitrogenUniRule annotation1
Binding sitei297FMN; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 66FMNUniRule annotation5
Nucleotide bindingi318 – 319FMNUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyrimidine biosynthesis
LigandFlavoprotein, FMN

Enzyme and pathway databases

BioCyciCBUR227377:G1G0B-962-MONOMER
UniPathwayiUPA00070; UER00946

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroorotate dehydrogenase (quinone)UniRule annotation (EC:1.3.5.2UniRule annotation)
Alternative name(s):
DHOdehaseUniRule annotation
Short name:
DHODUniRule annotation
Short name:
DHODaseUniRule annotation
Dihydroorotate oxidaseUniRule annotation
Gene namesi
Name:pyrDUniRule annotation
Ordered Locus Names:CBU_0971
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001484351 – 347Dihydroorotate dehydrogenase (quinone)Add BLAST347

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_0971

Structurei

3D structure databases

ProteinModelPortaliQ83CY0
SMRiQ83CY0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni111 – 115Substrate bindingUniRule annotation5
Regioni246 – 247Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QYT Bacteria
COG0167 LUCA
HOGENOMiHOG000225103
KOiK00254
OMAiLQNAMGF

Family and domain databases

CDDicd04738 DHOD_2_like, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00225 DHO_dh_type2, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR005720 Dihydroorotate_DH
IPR012135 Dihydroorotate_DH_1_2
IPR005719 Dihydroorotate_DH_2
IPR001295 Dihydroorotate_DH_CS
PfamiView protein in Pfam
PF01180 DHO_dh, 1 hit
PIRSFiPIRSF000164 DHO_oxidase, 1 hit
TIGRFAMsiTIGR01036 pyrD_sub2, 1 hit
PROSITEiView protein in PROSITE
PS00911 DHODEHASE_1, 1 hit
PS00912 DHODEHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q83CY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYRYLRPWL FKLEPETAHA LTLNCLKWFY CYWLINRRLQ RFPQKPTVVF
60 70 80 90 100
GIEFPNPVGL AAGLDKNGEY MDELLGLGFG FIEVGAVTPK PQPGNSKPRI
110 120 130 140 150
FRLPQARALI NRMGFNNLGV DYLVEQLKRR KVKGIVGVNI GKNLTTPLEK
160 170 180 190 200
AHEDYQNCFE KLYSYVDYVT INISSPNTPE LRQLQSERYL ADLLTRLKED
210 220 230 240 250
QRRLEDQYHK RVPLFLKIAP DLTPEEIQTI ATLALQHRIE GIVATNTSCS
260 270 280 290 300
RQGTEKLPNA NEAGGLSGKP LFPMTLQVVK QLHSFLGDEI PIVAVGGIFS
310 320 330 340
GENAQTLINA GARLVQLYTG LIYEGPELVK NIVEFLTLSR QTREGIN
Length:347
Mass (Da):39,314
Last modified:June 1, 2003 - v1
Checksum:i116F7122D9C3704D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA Translation: AAO90493.1
RefSeqiNP_819979.1, NC_002971.3
WP_005768550.1, NZ_LM994641.1

Genome annotation databases

EnsemblBacteriaiAAO90493; AAO90493; CBU_0971
GeneIDi1208866
31483697
KEGGicbu:CBU_0971
PATRICifig|227377.7.peg.968

Similar proteinsi

Entry informationi

Entry nameiPYRD_COXBU
AccessioniPrimary (citable) accession number: Q83CY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2003
Last modified: February 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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