Reviewed, UniProtKB/Swiss-Prot Q83CV9 (DEF1_COXBU)

Names and origin

Protein names

Recommended name:
    Peptide deformylase 1
      Short name=PDF 1
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase 1

Gene names

Name:	def1
Ordered Locus Names:	CBU_0993

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Peptide deformylase 1 HAMAP MF_00163		PRO_0000082776
Sites
	Active site	136	1	By similarity
	Metal binding	92	1	Iron By similarity
	Metal binding	135	1	Iron By similarity
	Metal binding	139	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83CV9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 320ACF14062D0D0E	FASTA	170	19,336
	10 20 30 40 50 60 MFKILQYPDP RLKTAAQRVE KFDDALQKMI DEMFETHYAA TNCAALAATQ LDMENPKHIT 70 80 90 100 110 120 VIDFSPNKDQ PLCLVNAEII ERSGEHTEEE GCMSVGGGTF EKVTRAAKIK VRARDRYGKP 130 140 150 160 170 VEFEADGFMA KCIQHELDHL NGIIFLDRLS TLKRGRIDKR LGKLRRQGKV

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO90514.1.
RefSeq	NP_820000.1.
3D structure databases
HSSP	HSSP built from PDB template 1N5N based on UniProtKB Q9I7A8.
ModBase	Search...
Genome annotation databases
GeneID	1208889.
GenomeReviews	Gene locus CBU_0993 in contig AE016828_GR.
KEGG	cbu:CBU_0993.
NMPDR	fig|227377.1.peg.944.
TIGR	CBU_0993.
Phylogenomic databases
HOGENOM	Q83CV9.
OMA	FASQRTA.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_0993-MON.
BRENDA	3.5.1.88. 256353.
Family and domain databases
HAMAP	MF_00163. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
ProDom	PD003844. Fmet_deformylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF1_COXBU

Accession

Primary (citable) accession number: Q83CV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)