Q83CV6 (CYOE_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protoheme IX farnesyltransferase
EC=2.5.1.-

Alternative name(s):
Heme B farnesyltransferase
Heme O synthase

Gene names

Name:	cyoE
Ordered Locus Names:	CBU_0996

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group By similarity. HAMAP-Rule MF_00154
Pathway	Porphyrin metabolism; heme O biosynthesis; heme O from protoheme: step 1/1. HAMAP-Rule MF_00154
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential HAMAP-Rule MF_00154.
Miscellaneous	Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature By similarity. HAMAP-Rule MF_00154
Sequence similarities	Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heme O biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protoheme IX farnesyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Protoheme IX farnesyltransferase HAMAP-Rule MF_00154		PRO_0000326889
Regions
	Transmembrane	28 – 48	21	Helical; Potential
	Transmembrane	52 – 72	21	Helical; Potential
	Transmembrane	102 – 122	21	Helical; Potential
	Transmembrane	123 – 143	21	Helical; Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Transmembrane	176 – 196	21	Helical; Potential
	Transmembrane	221 – 241	21	Helical; Potential
	Transmembrane	243 – 263	21	Helical; Potential
	Transmembrane	282 – 302	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q83CV6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4E36FD3728B88F1D	FASTA	305	34,456
	10 20 30 40 50 60 MRTRTEIVST TQTSATWRDY FQLCKPRVVL LMLLTAIVGM CLASPGIVSW RVFLFGNLGI 70 80 90 100 110 120 ALAASSAAAI NHLLEHHLDK LMRRTYRRPI VQGKINRKNA AIFAAILCIL SMIILIAFVN 130 140 150 160 170 180 LLTALLTFIT LIGYAGFYTL YLKHATPQNI VIGGLAGAAP PLLGWVAVTG HIDPPALILL 190 200 210 220 230 240 LIIFLWTPPH FWALAIHRID DYAKANIPML PNTHGIIYTK INILLYTLLL TAISFLPFVI 250 260 270 280 290 300 MTSGWIYFSS VCLLNLGFLY WAIRLLTSQR KEIPMRTFQY SIWYLMLLFT ALLVDHYVYL ALKLY

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO90517.1.
RefSeq	NP_820003.1. NC_002971.3.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_0996.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO90517; AAO90517; CBU_0996.
GeneID	1208892.
KEGG	cbu:CBU_0996.
PATRIC	17930711. VBICoxBur82552_0989.
Phylogenomic databases
eggNOG	COG0109.
HOGENOM	HOG000237291.
KO	K02301.
OMA	FCAIIGM.
ProtClustDB	PRK04375.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-987-MONOMER.
UniPathway	UPA00834; UER00712.
Family and domain databases
HAMAP	MF_00154. CyoE_CtaB.
InterPro	IPR006369. Protohaem_IX_farnesylTrfase. IPR000537. UbiA_prenyltransferase. [Graphical view]
Pfam	PF01040. UbiA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01473. cyoE_ctaB. 1 hit.
PROSITE	PS00943. UBIA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYOE_COXBU

Accession

Primary (citable) accession number: Q83CV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program