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Protein

Adenylosuccinate synthetase

Gene

purA

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.UniRule annotation

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase (purA)
  2. Adenylosuccinate lyase (purB)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei12Proton acceptorUniRule annotation1
Metal bindingi12MagnesiumUniRule annotation1
Metal bindingi39Magnesium; via carbonyl oxygenUniRule annotation1
Active sitei40Proton donorUniRule annotation1
Binding sitei128IMPUniRule annotation1
Binding sitei142IMP; shared with dimeric partnerUniRule annotation1
Binding sitei223IMPUniRule annotation1
Binding sitei238IMPUniRule annotation1
Binding sitei302IMPUniRule annotation1
Binding sitei304GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 17GTPUniRule annotation7
Nucleotide bindingi39 – 41GTPUniRule annotation3
Nucleotide bindingi330 – 332GTPUniRule annotation3
Nucleotide bindingi412 – 414GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00075; UER00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)
Short name:
AMPSaseUniRule annotation
Short name:
AdSSUniRule annotation
Alternative name(s):
IMP--aspartate ligaseUniRule annotation
Gene namesi
Name:purAUniRule annotation
Ordered Locus Names:CBU_0998
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

Q83CV4:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000951721 – 435Adenylosuccinate synthetaseAdd BLAST435

Proteomic databases

PRIDEiQ83CV4.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_0998.

Structurei

3D structure databases

ProteinModelPortaliQ83CV4.
SMRiQ83CV4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 15IMP bindingUniRule annotation4
Regioni37 – 40IMP bindingUniRule annotation4
Regioni298 – 304Substrate bindingUniRule annotation7

Sequence similaritiesi

Belongs to the adenylosuccinate synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C91. Bacteria.
COG0104. LUCA.
HOGENOMiHOG000260959.
KOiK01939.
OMAiGVSKAYT.

Family and domain databases

CDDicd03108. AdSS. 1 hit.
HAMAPiMF_00011. Adenylosucc_synth. 1 hit.
InterProiView protein in InterPro
IPR018220. Adenylosuccin_syn_GTP-bd.
IPR033128. Adenylosuccin_syn_Lys_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027417. P-loop_NTPase.
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiView protein in Pfam
PF00709. Adenylsucc_synt. 1 hit.
SMARTiView protein in SMART
SM00788. Adenylsucc_synt. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiView protein in PROSITE
PS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83CV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIVILGTQW GDEGKGKIVD MLTEDVAAVV RFQGGHNAGH TLIIDGEKTI
60 70 80 90 100
LRLIPSGILR EGVLCLIGNG VVLSPPALME EIEELNAKGI PVTEQLRISS
110 120 130 140 150
ACNLLLPYHV ALDKAREAEL GTKAIGTTGR GIGPAYEDKV ARRGIRAMDL
160 170 180 190 200
LHPDQLLEKI KKATAYHNIQ LEHYYHQTPL DYQSIYNQLM EFREKIKPMI
210 220 230 240 250
GDVSALLGNL RRQNKHIIFE GAQGSLLDID LGTYPYVTSS NTTAGSAATG
260 270 280 290 300
SGFGPLYFDR VLGITKAYVT RVGAGPFPTE LTNEEGKKMA KRGNEFGSVT
310 320 330 340 350
GRPRRCGWFD VISMRRTIQI NSLTGIVLTK LDVLDEFAKI HLCTAYRCDG
360 370 380 390 400
EVVNEPPFDQ SLLESCEPVY EEMPGWQTST YGLTDYSEMP KEARNYISRL
410 420 430
EELLGVPITI ISTGPDRKHT IVRQAVFNQV ITAKG
Length:435
Mass (Da):47,989
Last modified:June 1, 2003 - v1
Checksum:i8F836A0BBC28D306
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90519.1.
RefSeqiNP_820005.1. NC_002971.3.
WP_010957943.1. NC_002971.4.

Genome annotation databases

EnsemblBacteriaiAAO90519; AAO90519; CBU_0998.
GeneIDi1208894.
KEGGicbu:CBU_0998.
PATRICifig|227377.7.peg.991.

Similar proteinsi

Entry informationi

Entry nameiPURA_COXBU
AccessioniPrimary (citable) accession number: Q83CV4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2003
Last modified: June 7, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families