Q83CG2 (TRPA_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase alpha chain
EC=4.2.1.20

Gene names

Name:	trpA
Ordered Locus Names:	CBU_1156

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate By similarity. HAMAP-Rule MF_00131
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP-Rule MF_00131
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP-Rule MF_00131
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpA family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	tryptophan synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Tryptophan synthase alpha chain HAMAP-Rule MF_00131		PRO_0000098774
Sites
	Active site	44	1	Proton acceptor By similarity
	Active site	55	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83CG2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E143E41C19C0E858	FASTA	267	28,924
	10 20 30 40 50 60 MNRIEQQFKK SPAYVAYLTA GDGGLERSLE SLLALAKGGV NILEVGVPFS DPVADGPVIQ 70 80 90 100 110 120 EASIRALAQG TTLHDVLTLI TSFRQHSEIP IILFTYFNPL LAAGDKIYQQ MKSAGVDGCL 130 140 150 160 170 180 VVDLPVEEAA PHLTACKTAK IAPILLISPS TTQERLKKIN EHGEGMLYYV CRPGTTGVRA 190 200 210 220 230 240 TLPENFPAKM NQIKSMTSLP IVTGFGIANR KMAAQALQYA DGFVIGSLFV KAIAEGISKN 250 260 ALTRLAQSLN PHYPNLRLIT PFRKKTF

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO90667.1.
RefSeq	NP_820153.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83CG2.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1156.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO90667; AAO90667; CBU_1156.
GeneID	1209058.
KEGG	cbu:CBU_1156.
PATRIC	17931051. VBICoxBur82552_1153.
Phylogenomic databases
eggNOG	COG0159.
HOGENOM	HOG000223814.
KO	K01695.
OMA	VFICPPN.
ProtClustDB	PRK13111.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1145-MONOMER.
UniPathway	UPA00035; UER00044.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00131. Trp_synth_alpha.
InterPro	IPR013785. Aldolase_TIM. IPR011060. RibuloseP-bd_barrel. IPR018204. Trp_synthase_alpha_AS. IPR002028. Trp_synthase_suA. [Graphical view]
Pfam	PF00290. Trp_syntA. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00262. trpA. 1 hit.
PROSITE	PS00167. TRP_SYNTHASE_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPA_COXBU

Accession

Primary (citable) accession number: Q83CG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program