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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei269ATPUniRule annotation1
Binding sitei290ATP; via amide nitrogenUniRule annotation1
Binding sitei299ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi186 – 187ATPUniRule annotation2
Nucleotide bindingi211 – 212ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:CBU_1181
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001548361 – 392Probable tRNA sulfurtransferaseAdd BLAST392

Proteomic databases

PRIDEiQ83CD9.

Interactioni

Protein-protein interaction databases

STRINGi227377.CBU_1181.

Structurei

3D structure databases

ProteinModelPortaliQ83CD9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 166THUMPUniRule annotationAdd BLAST108

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D8I. Bacteria.
COG0301. LUCA.
HOGENOMiHOG000227470.
KOiK03151.
OMAiEAAYISH.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83CD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVILIKYG EIALKGKNRH LFESSIIENI RLAIGEGAPP IEQCRGRLYL
60 70 80 90 100
QLTTEKDISC YREALKRVFG VVGFALAYRL NLEINLEEME EVLIKHLRKL
110 120 130 140 150
ESKSLAFRVD TRRTVKSFPM DSMEINKKLG ALILQHFPKW QVNLNNPELT
160 170 180 190 200
IFIEVRDEGL FIYTTEDHED GLGGLPVGVG GRGLLLLSGG IDSPVAGWTL
210 220 230 240 250
LKRGMMIDAV YFHSFPYTGE KAKEKVIDLA RVLTSWKLRA INLHIPYFTK
260 270 280 290 300
IQETVNKMCP ESTWTIIHRR FMMRIAEKLT KSTYHTLITG ENLGQVASQT
310 320 330 340 350
IQNIAVINQA TNLPILRPLI SFDKNDIIKI AEKIGTFRIS KRPYEDCCAL
360 370 380 390
FAPKNPETKA KEEAILKAEE NLPLNELINE ALEKMETLRI KN
Length:392
Mass (Da):44,677
Last modified:June 1, 2003 - v1
Checksum:iECE42AFA1F6E0AE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90690.1.
RefSeqiNP_820176.1. NC_002971.3.
WP_010958056.1. NC_002971.3.

Genome annotation databases

EnsemblBacteriaiAAO90690; AAO90690; CBU_1181.
GeneIDi1209084.
KEGGicbu:CBU_1181.
PATRICi17931103. VBICoxBur82552_1178.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90690.1.
RefSeqiNP_820176.1. NC_002971.3.
WP_010958056.1. NC_002971.3.

3D structure databases

ProteinModelPortaliQ83CD9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227377.CBU_1181.

Proteomic databases

PRIDEiQ83CD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO90690; AAO90690; CBU_1181.
GeneIDi1209084.
KEGGicbu:CBU_1181.
PATRICi17931103. VBICoxBur82552_1178.

Phylogenomic databases

eggNOGiENOG4105D8I. Bacteria.
COG0301. LUCA.
HOGENOMiHOG000227470.
KOiK03151.
OMAiEAAYISH.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHII_COXBU
AccessioniPrimary (citable) accession number: Q83CD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.