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Q83CD9

- THII_COXBU

UniProt

Q83CD9 - THII_COXBU

Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

    Catalytic activityi

    L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei269 – 2691ATPUniRule annotation
    Binding sitei290 – 2901ATP; via amide nitrogenUniRule annotation
    Binding sitei299 – 2991ATPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi186 – 1872ATPUniRule annotation
    Nucleotide bindingi211 – 2122ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfurtransferase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. tRNA thio-modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciCBUR227377:GJ7S-1167-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
    Thiamine biosynthesis protein ThiIUniRule annotation
    tRNA 4-thiouridine synthaseUniRule annotation
    Gene namesi
    Name:thiIUniRule annotation
    Ordered Locus Names:CBU_1181
    OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
    Taxonomic identifieri227377 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
    ProteomesiUP000002671: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 392392Probable tRNA sulfurtransferasePRO_0000154836Add
    BLAST

    Proteomic databases

    PRIDEiQ83CD9.

    Interactioni

    Protein-protein interaction databases

    STRINGi227377.CBU_1181.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83CD9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini59 – 166108THUMPUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThiI family.UniRule annotation
    Contains 1 THUMP domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0301.
    HOGENOMiHOG000227470.
    KOiK03151.
    OMAiAQMKGPD.
    OrthoDBiEOG6TBHGR.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00021. ThiI.
    InterProiIPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view]
    PfamiPF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view]
    SMARTiSM00981. THUMP. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
    PROSITEiPS51165. THUMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q83CD9-1 [UniParc]FASTAAdd to Basket

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    MKKVILIKYG EIALKGKNRH LFESSIIENI RLAIGEGAPP IEQCRGRLYL    50
    QLTTEKDISC YREALKRVFG VVGFALAYRL NLEINLEEME EVLIKHLRKL 100
    ESKSLAFRVD TRRTVKSFPM DSMEINKKLG ALILQHFPKW QVNLNNPELT 150
    IFIEVRDEGL FIYTTEDHED GLGGLPVGVG GRGLLLLSGG IDSPVAGWTL 200
    LKRGMMIDAV YFHSFPYTGE KAKEKVIDLA RVLTSWKLRA INLHIPYFTK 250
    IQETVNKMCP ESTWTIIHRR FMMRIAEKLT KSTYHTLITG ENLGQVASQT 300
    IQNIAVINQA TNLPILRPLI SFDKNDIIKI AEKIGTFRIS KRPYEDCCAL 350
    FAPKNPETKA KEEAILKAEE NLPLNELINE ALEKMETLRI KN 392
    Length:392
    Mass (Da):44,677
    Last modified:June 1, 2003 - v1
    Checksum:iECE42AFA1F6E0AE7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO90690.1.
    RefSeqiNP_820176.1. NC_002971.3.

    Genome annotation databases

    EnsemblBacteriaiAAO90690; AAO90690; CBU_1181.
    GeneIDi1209084.
    KEGGicbu:CBU_1181.
    PATRICi17931103. VBICoxBur82552_1178.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO90690.1 .
    RefSeqi NP_820176.1. NC_002971.3.

    3D structure databases

    ProteinModelPortali Q83CD9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 227377.CBU_1181.

    Proteomic databases

    PRIDEi Q83CD9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO90690 ; AAO90690 ; CBU_1181 .
    GeneIDi 1209084.
    KEGGi cbu:CBU_1181.
    PATRICi 17931103. VBICoxBur82552_1178.

    Phylogenomic databases

    eggNOGi COG0301.
    HOGENOMi HOG000227470.
    KOi K03151.
    OMAi AQMKGPD.
    OrthoDBi EOG6TBHGR.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci CBUR227377:GJ7S-1167-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00021. ThiI.
    InterProi IPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view ]
    Pfami PF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view ]
    SMARTi SM00981. THUMP. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00342. TIGR00342. 1 hit.
    PROSITEi PS51165. THUMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RSA 493 / Nine Mile phase I.

    Entry informationi

    Entry nameiTHII_COXBU
    AccessioniPrimary (citable) accession number: Q83CD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Coxiella burnetii
      Coxiella burnetii (strain RSA 493): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3