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Q83CD9 (THII_COXBU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable tRNA sulfurtransferase

EC=2.8.1.4
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase
Gene names
Name:thiI
Ordered Locus Names:CBU_1181
OrganismCoxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]
Taxonomic identifier227377 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS By similarity. HAMAP-Rule MF_00021

Catalytic activity

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide. HAMAP-Rule MF_00021

[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP. HAMAP-Rule MF_00021

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP-Rule MF_00021

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00021.

Sequence similarities

Belongs to the ThiI family.

Contains 1 THUMP domain.

Ontologies

Keywords
   Biological processThiamine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
tRNA-binding
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA thio-modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

thiamine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

thiamine diphosphate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

sulfurtransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 392392Probable tRNA sulfurtransferase HAMAP-Rule MF_00021
PRO_0000154836

Regions

Domain59 – 166108THUMP
Nucleotide binding186 – 1872ATP By similarity
Nucleotide binding211 – 2122ATP By similarity

Sites

Binding site2691ATP By similarity
Binding site2901ATP; via amide nitrogen By similarity
Binding site2991ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83CD9 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: ECE42AFA1F6E0AE7

FASTA39244,677
        10         20         30         40         50         60 
MKKVILIKYG EIALKGKNRH LFESSIIENI RLAIGEGAPP IEQCRGRLYL QLTTEKDISC 

        70         80         90        100        110        120 
YREALKRVFG VVGFALAYRL NLEINLEEME EVLIKHLRKL ESKSLAFRVD TRRTVKSFPM 

       130        140        150        160        170        180 
DSMEINKKLG ALILQHFPKW QVNLNNPELT IFIEVRDEGL FIYTTEDHED GLGGLPVGVG 

       190        200        210        220        230        240 
GRGLLLLSGG IDSPVAGWTL LKRGMMIDAV YFHSFPYTGE KAKEKVIDLA RVLTSWKLRA 

       250        260        270        280        290        300 
INLHIPYFTK IQETVNKMCP ESTWTIIHRR FMMRIAEKLT KSTYHTLITG ENLGQVASQT 

       310        320        330        340        350        360 
IQNIAVINQA TNLPILRPLI SFDKNDIIKI AEKIGTFRIS KRPYEDCCAL FAPKNPETKA 

       370        380        390 
KEEAILKAEE NLPLNELINE ALEKMETLRI KN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016828 Genomic DNA. Translation: AAO90690.1.
RefSeqNP_820176.1. NC_002971.3.

3D structure databases

ProteinModelPortalQ83CD9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227377.CBU_1181.

Proteomic databases

PRIDEQ83CD9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO90690; AAO90690; CBU_1181.
GeneID1209084.
KEGGcbu:CBU_1181.
PATRIC17931103. VBICoxBur82552_1178.

Phylogenomic databases

eggNOGCOG0301.
HOGENOMHOG000227470.
KOK03151.
OMAAQKIDTF.
OrthoDBEOG6TBHGR.
ProtClustDBPRK01565.

Enzyme and pathway databases

BioCycCBUR227377:GJ7S-1167-MONOMER.
UniPathwayUPA00060.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00021. ThiI.
InterProIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_C_dom.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsTIGR00342. TIGR00342. 1 hit.
PROSITEPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTHII_COXBU
AccessionPrimary (citable) accession number: Q83CD9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Coxiella burnetii

Coxiella burnetii (strain RSA 493): entries and gene names