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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei269ATPUniRule annotation1
Binding sitei290ATP; via amide nitrogenUniRule annotation1
Binding sitei299ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi186 – 187ATPUniRule annotation2
Nucleotide bindingi211 – 212ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processThiamine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCBUR227377:G1G0B-1164-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:CBU_1181
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001548361 – 392Probable tRNA sulfurtransferaseAdd BLAST392

Proteomic databases

PRIDEiQ83CD9

Interactioni

Protein-protein interaction databases

STRINGi227377.CBU_1181

Structurei

3D structure databases

ProteinModelPortaliQ83CD9
SMRiQ83CD9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 166THUMPUniRule annotationAdd BLAST108

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D8I Bacteria
COG0301 LUCA
HOGENOMiHOG000227470
KOiK03151
OMAiLENMYIV

Family and domain databases

CDDicd01712 ThiI, 1 hit
Gene3Di3.40.50.6201 hit
HAMAPiMF_00021 ThiI, 1 hit
InterProiView protein in InterPro
IPR014729 Rossmann-like_a/b/a_fold
IPR020536 ThiI_AANH
IPR004114 THUMP_dom
IPR003720 tRNA_STrfase
PfamiView protein in Pfam
PF02568 ThiI, 1 hit
PF02926 THUMP, 1 hit
SMARTiView protein in SMART
SM00981 THUMP, 1 hit
TIGRFAMsiTIGR00342 TIGR00342, 1 hit
PROSITEiView protein in PROSITE
PS51165 THUMP, 1 hit

Sequencei

Sequence statusi: Complete.

Q83CD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKVILIKYG EIALKGKNRH LFESSIIENI RLAIGEGAPP IEQCRGRLYL
60 70 80 90 100
QLTTEKDISC YREALKRVFG VVGFALAYRL NLEINLEEME EVLIKHLRKL
110 120 130 140 150
ESKSLAFRVD TRRTVKSFPM DSMEINKKLG ALILQHFPKW QVNLNNPELT
160 170 180 190 200
IFIEVRDEGL FIYTTEDHED GLGGLPVGVG GRGLLLLSGG IDSPVAGWTL
210 220 230 240 250
LKRGMMIDAV YFHSFPYTGE KAKEKVIDLA RVLTSWKLRA INLHIPYFTK
260 270 280 290 300
IQETVNKMCP ESTWTIIHRR FMMRIAEKLT KSTYHTLITG ENLGQVASQT
310 320 330 340 350
IQNIAVINQA TNLPILRPLI SFDKNDIIKI AEKIGTFRIS KRPYEDCCAL
360 370 380 390
FAPKNPETKA KEEAILKAEE NLPLNELINE ALEKMETLRI KN
Length:392
Mass (Da):44,677
Last modified:June 1, 2003 - v1
Checksum:iECE42AFA1F6E0AE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA Translation: AAO90690.1
RefSeqiNP_820176.1, NC_002971.3
WP_010958056.1, NC_002971.4

Genome annotation databases

EnsemblBacteriaiAAO90690; AAO90690; CBU_1181
GeneIDi1209084
31483884
KEGGicbu:CBU_1181
PATRICifig|227377.7.peg.1178

Similar proteinsi

Entry informationi

Entry nameiTHII_COXBU
AccessioniPrimary (citable) accession number: Q83CD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2003
Last modified: February 28, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome