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Q83CA6

- DAPA_COXBU

UniProt

Q83CA6 - DAPA_COXBU

Protein

4-hydroxy-tetrahydrodipicolinate synthase

Gene

dapA

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).UniRule annotation

    Catalytic activityi

    Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei44 – 441Part of a proton relay during catalysisUniRule annotation
    Binding sitei45 – 451PyruvateUniRule annotation
    Sitei107 – 1071Part of a proton relay during catalysisUniRule annotation
    Active sitei133 – 1331Proton donor/acceptorUniRule annotation
    Active sitei161 – 1611Schiff-base intermediate with substrateUniRule annotation
    Binding sitei200 – 2001Pyruvate; via carbonyl oxygenUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
    2. amine-lyase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
    2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciCBUR227377:GJ7S-1209-MONOMER.
    UniPathwayiUPA00034; UER00017.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation (EC:4.3.3.7UniRule annotation)
    Short name:
    HTPA synthaseUniRule annotation
    Gene namesi
    Name:dapAUniRule annotation
    Ordered Locus Names:CBU_1222
    OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
    Taxonomic identifieri227377 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
    ProteomesiUP000002671: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2892894-hydroxy-tetrahydrodipicolinate synthasePRO_1000050182Add
    BLAST

    Proteomic databases

    PRIDEiQ83CA6.

    Interactioni

    Subunit structurei

    Homotetramer; dimer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi227377.CBU_1222.

    Structurei

    3D structure databases

    ProteinModelPortaliQ83CA6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DapA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0329.
    HOGENOMiHOG000173604.
    KOiK01714.
    OMAiMLYSGDD.
    OrthoDBiEOG6W7235.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00418. DapA.
    InterProiIPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    IPR020624. Dihydrodipicolinate_synth_CS.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.
    TIGRFAMsiTIGR00674. dapA. 1 hit.
    PROSITEiPS00665. DHDPS_1. 1 hit.
    PS00666. DHDPS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q83CA6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFNGSLVALV TPMQENGEID YSNLKELVEW HLENDTDGLV ILGTTGESPT    50
    ITAEERHKII RQVVDQVNKK IPIIVGTGAN STVHTIEMTQ QAMELGADAA 100
    LIVTPYYNKP TQEGLFQYFK TIAEAVPIAQ ILYNVPSRTA CDLLPETVIR 150
    IAKCSNVVGL KEATGDIQRV KQLKAEDLDL LSGDDKTAMD FMLAGGKGVI 200
    SVVANVVPKP YHAFCITAVS GNVELAKKEN DQLSPLYDSL FVESNPIPVK 250
    WALSQMGVIP KGIRLPLTPL SERYHAKVRE SLQQVGIKC 289
    Length:289
    Mass (Da):31,613
    Last modified:June 1, 2003 - v1
    Checksum:iE88266A24A2CEC6F
    GO

    Sequence cautioni

    The sequence AAO90731.2 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO90731.2. Different initiation.
    RefSeqiNP_820217.2. NC_002971.3.

    Genome annotation databases

    EnsemblBacteriaiAAO90731; AAO90731; CBU_1222.
    GeneIDi1209127.
    KEGGicbu:CBU_1222.
    PATRICi17931181. VBICoxBur82552_1215.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO90731.2 . Different initiation.
    RefSeqi NP_820217.2. NC_002971.3.

    3D structure databases

    ProteinModelPortali Q83CA6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 227377.CBU_1222.

    Proteomic databases

    PRIDEi Q83CA6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO90731 ; AAO90731 ; CBU_1222 .
    GeneIDi 1209127.
    KEGGi cbu:CBU_1222.
    PATRICi 17931181. VBICoxBur82552_1215.

    Phylogenomic databases

    eggNOGi COG0329.
    HOGENOMi HOG000173604.
    KOi K01714.
    OMAi MLYSGDD.
    OrthoDBi EOG6W7235.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00017 .
    BioCyci CBUR227377:GJ7S-1209-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00418. DapA.
    InterProi IPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    IPR020624. Dihydrodipicolinate_synth_CS.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    TIGRFAMsi TIGR00674. dapA. 1 hit.
    PROSITEi PS00665. DHDPS_1. 1 hit.
    PS00666. DHDPS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RSA 493 / Nine Mile phase I.

    Entry informationi

    Entry nameiDAPA_COXBU
    AccessioniPrimary (citable) accession number: Q83CA6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Coxiella burnetii
      Coxiella burnetii (strain RSA 493): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3