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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.

Cofactori

Fe2+Note: Binds 1 Fe2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi112Iron1
Metal bindingi116Iron1
Binding sitei168Substrate1
Binding sitei181Substrate; via amide nitrogen1
Binding sitei273Substrate1
Metal bindingi301Iron1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandIron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferase (EC:2.3.1.234)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD
tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
Gene namesi
Name:tsaD
Synonyms:gcp
Ordered Locus Names:CBU_1240
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003033401 – 339tRNA N6-adenosine threonylcarbamoyltransferaseAdd BLAST339

Proteomic databases

PRIDEiQ83C88.

Interactioni

Protein-protein interaction databases

STRINGi227377.CBU_1240.

Structurei

3D structure databases

ProteinModelPortaliQ83C88.
SMRiQ83C88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 139Substrate binding5

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
KOiK01409.
OMAiHLEGHIY.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiView protein in InterPro
IPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
PANTHERiPTHR11735. PTHR11735. 1 hit.
PTHR11735:SF6. PTHR11735:SF6. 1 hit.
PfamiView protein in Pfam
PF00814. Peptidase_M22. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
PROSITEiView protein in PROSITE
PS01016. GLYCOPROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83C88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCVLGVETS CDETAVALYD GERGLLAHRV YSQIAIHAEY GGVVPELASR
60 70 80 90 100
DHIRKILPLI KAALDDAALS KENIDGIAYT KGPGLIGALM VGASVAKSLA
110 120 130 140 150
YAWRVPVVGV HHMEAHLMAL QLEESRPAYP FIALLVSGGH TMLVHVEQPG
160 170 180 190 200
RYKILGESVD DAAGEAFDKT AKLLGLPYPG GPALARLAEQ GEPKRFIFPR
210 220 230 240 250
PMVNQPHLNF SFSGLKTHAV NCFKQYGGEE QTRADIACAF ENAVVDTLII
260 270 280 290 300
KCLRALEKTG INTLVLVGGV AANKKLRERL GQVAVKRAAQ IYYPRQEFCT
310 320 330
DNGAMVAYTG WLRLNAGEKE DKIIRVKPRW SMAELNIIN
Length:339
Mass (Da):36,955
Last modified:June 1, 2003 - v1
Checksum:iD964BA6EECDDCEFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90749.1.
RefSeqiNP_820235.1. NC_002971.3.
WP_010958097.1. NC_002971.4.

Genome annotation databases

EnsemblBacteriaiAAO90749; AAO90749; CBU_1240.
GeneIDi1209145.
KEGGicbu:CBU_1240.
PATRICifig|227377.7.peg.1231.

Similar proteinsi

Entry informationi

Entry nameiTSAD_COXBU
AccessioniPrimary (citable) accession number: Q83C88
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2003
Last modified: November 22, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families