Reviewed, UniProtKB/Swiss-Prot Q83C87 (MDH_COXBU)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	CBU_1241

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01517
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Malate dehydrogenase HAMAP MF_01517		PRO_0000113366
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity
	Nucleotide binding	129 – 131	3	NAD By similarity
Sites
	Active site	187	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	105	1	NAD By similarity
	Binding site	112	1	NAD By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83C87-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 6CA91ACB6E43ACB2	FASTA	328	35,469
	10 20 30 40 50 60 MAKHVKVAVT GAAGQIGYAL LFRLASGQAF GLDTTVDLHL LEIEPALPAL KGVVMELEDC 70 80 90 100 110 120 AFPLLRNMVV TSDPRVAFND VNWALLVGAA PRKAGMERKD LLEKNGSIFA GQGKAINENA 130 140 150 160 170 180 ASDVRIFVVG NPCNTNCLIA MNNAPDIPKD RFYAMTRLDQ NRAIGQLALK AGVDVPSVKN 190 200 210 220 230 240 MIIWGNHSST QYPDFYHATI DGKPATEVIR DKNWLLNDFI SVIQQRGAAV IKARGASSAA 250 260 270 280 290 300 SAANAALDSV WSLINTTPAD DNYSVALCAQ GQYGVDEGLI FSFPCRTENG VVSVIEEIEH 310 320 NEFGQQKLKE TLDELREERD AVEALGLI

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO90750.1.
RefSeq	NP_820236.1.
3D structure databases
HSSP	HSSP built from PDB template 1BMD based on UniProtKB P10584.
ModBase	Search...
Genome annotation databases
GeneID	1209146.
GenomeReviews	Gene locus CBU_1241 in contig AE016828_GR.
KEGG	cbu:CBU_1241.
NMPDR	fig|227377.1.peg.1180.
TIGR	CBU_1241.
Phylogenomic databases
HOGENOM	Q83C87.
OMA	Q83C87. MIIWGNH.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_1241-MON.
BRENDA	1.1.1.37. 256353.
Family and domain databases
HAMAP	MF_01517. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010945. Malate_DH_SF1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
ProDom	PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_COXBU

Accession

Primary (citable) accession number: Q83C87

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)