Q83C41 (GRPE_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein GrpE

Alternative name(s):
HSP-70 cofactor

Gene names

Name:	grpE
Ordered Locus Names:	CBU_1293

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding By similarity. HAMAP-Rule MF_01151
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GrpE family.
Sequence caution	The sequence AAO90799.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	adenyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Protein GrpE HAMAP-Rule MF_01151		PRO_0000113779

Sequences

	Sequence		Length	Mass (Da)
	Q83C41 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9A0B557667133AEF	FASTA	204	22,952
	10 20 30 40 50 60 MSSKNNPESE TKAKNKWEKV MEAEEEQEEG RGDGSQEMEP HREGLEFPSR EKLEGQLTRM 70 80 90 100 110 120 ERKVDEYKTQ YLRAQAEMDN LRKRIEREKA DIIKFGSKQL ITDLLPVADS LIHGLESPAS 130 140 150 160 170 180 EDPQVKSMRD GMSLTLDLLH NTLAKHGVQV INPNPGDPFD PALHEAMSVQ AVPDAKPDTI 190 200 IQVLQKGYQL NGRVLRAARV IVAG

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO90799.2. Different initiation.
RefSeq	NP_820285.2. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83C41.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1293.
Proteomic databases
PRIDE	Q83C41.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO90799; AAO90799; CBU_1293.
GeneID	1209198.
KEGG	cbu:CBU_1293.
PATRIC	17931327. VBICoxBur82552_1288.
Phylogenomic databases
eggNOG	COG0576.
HOGENOM	HOG000252084.
KO	K03687.
OMA	EQLKETM.
ProtClustDB	PRK14154.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1281-MONOMER.
Family and domain databases
Gene3D	2.30.22.10. 1 hit. 3.90.20.20. 1 hit.
HAMAP	MF_01151. GrpE.
InterPro	IPR000740. GrpE. IPR013805. GrpE_coiled_coil. IPR009012. GrpE_head. [Graphical view]
PANTHER	PTHR21237. PTHR21237. 1 hit.
Pfam	PF01025. GrpE. 1 hit. [Graphical view]
PRINTS	PR00773. GRPEPROTEIN.
SUPFAM	SSF51064. GrpE_head. 1 hit.
PROSITE	PS01071. GRPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRPE_COXBU

Accession

Primary (citable) accession number: Q83C41

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program