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Q83C10 (SYT_COXBU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Threonine--tRNA ligase

EC=6.1.1.3
Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS
Gene names
Name:thrS
Ordered Locus Names:CBU_1326
OrganismCoxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]
Taxonomic identifier227377 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella

Protein attributes

Sequence length651 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP-Rule MF_00184

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00184

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00184

Subcellular location

Cytoplasm HAMAP-Rule MF_00184.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAO90830.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processthreonyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

threonine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 651651Threonine--tRNA ligase HAMAP-Rule MF_00184
PRO_0000100971

Regions

Region242 – 533292Catalytic HAMAP-Rule MF_00184

Sites

Metal binding3331Zinc; catalytic By similarity
Metal binding3841Zinc; catalytic By similarity
Metal binding5101Zinc; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83C10 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 8BADE57CA2D780D2

FASTA65173,784
        10         20         30         40         50         60 
MPTIQLPDGS VKQFSKPVSV QAVAESIGTS LAKPALAGKI DDHLVDVSFI IDKDVSLRII 

        70         80         90        100        110        120 
TEKDPEGLEV IRHSAAHLLA HAVKELFPKA EVTIGPVVED GFYYDFAFER SFTPEDLEKI 

       130        140        150        160        170        180 
EEKMRSLAKA DLAVERRVLS RDEALTLFKK MGERYKVEII RDIPKEEILT AYQQGDFIDL 

       190        200        210        220        230        240 
CRGPHVPRTG MLKAFKLTKL AGAYWRGDSN NEMLQRIYGT AWADTKALKA YLYRLEEAEK 

       250        260        270        280        290        300 
RDHRLLAKKM DLFHFQPESP GNVFWHPNGW SIILQMREYI RHITHKYGYQ EVHTPQLIDA 

       310        320        330        340        350        360 
SLWDKSGHLE KFGDDIFSLP LEPQQYVIKP MSCPAHVQIF NQGVKSYRDL PLRYAEFGAC 

       370        380        390        400        410        420 
HRNEPSGTLH GLMRLRGFVQ DDAHIFCTED QIQSEVSAFI DQLHEVYADF GFTEVIHKLS 

       430        440        450        460        470        480 
TRPEKRVGSD EVWTKAEQAL AEALNRKGVE WDILPGEGAF YGPKIEFSLR DCLGRIWQCG 

       490        500        510        520        530        540 
TVQVDFSVPE RLGAHYIAED GSKKPPVMIH RAILGSFERF LGILLEESAG KLPLWLAPVQ 

       550        560        570        580        590        600 
VVVMNITDRQ ADYVGQTVEN LQNLGIRAHS DLRNEKIGFK IREHTIARVP YLVVIGDREV 

       610        620        630        640        650 
ADKTLSVRAL EDEASTTITL EEFARQLKAE ISQRSRKSPA PSPLFPVGGE S 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016828 Genomic DNA. Translation: AAO90830.2. Different initiation.
RefSeqNP_820316.2. NC_002971.3.

3D structure databases

ProteinModelPortalQ83C10.
SMRQ83C10. Positions 241-637.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227377.CBU_1326.

Proteomic databases

PRIDEQ83C10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO90830; AAO90830; CBU_1326.
GeneID1209232.
KEGGcbu:CBU_1326.
PATRIC17931387. VBICoxBur82552_1317.

Phylogenomic databases

eggNOGCOG0441.
HOGENOMHOG000003880.
KOK01868.
OMACVGHIKV.
OrthoDBEOG61KBFJ.
ProtClustDBPRK00413.

Enzyme and pathway databases

BioCycCBUR227377:GJ7S-1317-MONOMER.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPMF_00184. Thr_tRNA_synth.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSPR01047. TRNASYNTHTHR.
SMARTSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00418. thrS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYT_COXBU
AccessionPrimary (citable) accession number: Q83C10
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Coxiella burnetii

Coxiella burnetii (strain RSA 493): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries