Q83C10 (SYT_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	CBU_1326

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP-Rule MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm HAMAP-Rule MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAO90830.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Threonine--tRNA ligase HAMAP-Rule MF_00184		PRO_0000100971
Regions
	Region	242 – 533	292	Catalytic HAMAP-Rule MF_00184
Sites
	Metal binding	333	1	Zinc; catalytic By similarity
	Metal binding	384	1	Zinc; catalytic By similarity
	Metal binding	510	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83C10 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8BADE57CA2D780D2	FASTA	651	73,784
	10 20 30 40 50 60 MPTIQLPDGS VKQFSKPVSV QAVAESIGTS LAKPALAGKI DDHLVDVSFI IDKDVSLRII 70 80 90 100 110 120 TEKDPEGLEV IRHSAAHLLA HAVKELFPKA EVTIGPVVED GFYYDFAFER SFTPEDLEKI 130 140 150 160 170 180 EEKMRSLAKA DLAVERRVLS RDEALTLFKK MGERYKVEII RDIPKEEILT AYQQGDFIDL 190 200 210 220 230 240 CRGPHVPRTG MLKAFKLTKL AGAYWRGDSN NEMLQRIYGT AWADTKALKA YLYRLEEAEK 250 260 270 280 290 300 RDHRLLAKKM DLFHFQPESP GNVFWHPNGW SIILQMREYI RHITHKYGYQ EVHTPQLIDA 310 320 330 340 350 360 SLWDKSGHLE KFGDDIFSLP LEPQQYVIKP MSCPAHVQIF NQGVKSYRDL PLRYAEFGAC 370 380 390 400 410 420 HRNEPSGTLH GLMRLRGFVQ DDAHIFCTED QIQSEVSAFI DQLHEVYADF GFTEVIHKLS 430 440 450 460 470 480 TRPEKRVGSD EVWTKAEQAL AEALNRKGVE WDILPGEGAF YGPKIEFSLR DCLGRIWQCG 490 500 510 520 530 540 TVQVDFSVPE RLGAHYIAED GSKKPPVMIH RAILGSFERF LGILLEESAG KLPLWLAPVQ 550 560 570 580 590 600 VVVMNITDRQ ADYVGQTVEN LQNLGIRAHS DLRNEKIGFK IREHTIARVP YLVVIGDREV 610 620 630 640 650 ADKTLSVRAL EDEASTTITL EEFARQLKAE ISQRSRKSPA PSPLFPVGGE S

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO90830.2. Different initiation.
RefSeq	NP_820316.2. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83C10.
SMR	Q83C10. Positions 241-637.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1326.
Proteomic databases
PRIDE	Q83C10.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO90830; AAO90830; CBU_1326.
GeneID	1209232.
KEGG	cbu:CBU_1326.
PATRIC	17931387. VBICoxBur82552_1317.
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HOG000003880.
KO	K01868.
OMA	KITYKIR.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1317-MONOMER.
Family and domain databases
Gene3D	3.10.20.30. 1 hit. 3.40.50.800. 1 hit.
HAMAP	MF_00184. Thr_tRNA_synth.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_dom. IPR012676. TGS-like. IPR002320. Thr-tRNA-ligase_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_COXBU

Accession

Primary (citable) accession number: Q83C10

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program