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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).UniRule annotation

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi333ZincUniRule annotation1
Metal bindingi384Zinc; via tele nitrogenUniRule annotation1
Metal bindingi510Zinc; via pros nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:CBU_1326
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001009711 – 651Threonine--tRNA ligaseAdd BLAST651

Proteomic databases

PRIDEiQ83C10.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_1326.

Structurei

3D structure databases

ProteinModelPortaliQ83C10.
SMRiQ83C10.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni242 – 533CatalyticUniRule annotationAdd BLAST292

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C22. Bacteria.
COG0441. LUCA.
HOGENOMiHOG000003880.
KOiK01868.
OMAiFYYDFAY.

Family and domain databases

CDDicd00771. ThrRS_core. 1 hit.
Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth. 1 hit.
InterProiView protein in InterPro
IPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR036621. Anticodon-bd_dom_sf.
IPR012675. Beta-grasp_dom_sf.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR033728. ThrRS_core.
IPR012947. tRNA_SAD.
PANTHERiPTHR11451. PTHR11451. 1 hit.
PfamiView protein in Pfam
PF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiView protein in SMART
SM00863. tRNA_SAD. 1 hit.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiView protein in PROSITE
PS50862. AA_TRNA_LIGASE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83C10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTIQLPDGS VKQFSKPVSV QAVAESIGTS LAKPALAGKI DDHLVDVSFI
60 70 80 90 100
IDKDVSLRII TEKDPEGLEV IRHSAAHLLA HAVKELFPKA EVTIGPVVED
110 120 130 140 150
GFYYDFAFER SFTPEDLEKI EEKMRSLAKA DLAVERRVLS RDEALTLFKK
160 170 180 190 200
MGERYKVEII RDIPKEEILT AYQQGDFIDL CRGPHVPRTG MLKAFKLTKL
210 220 230 240 250
AGAYWRGDSN NEMLQRIYGT AWADTKALKA YLYRLEEAEK RDHRLLAKKM
260 270 280 290 300
DLFHFQPESP GNVFWHPNGW SIILQMREYI RHITHKYGYQ EVHTPQLIDA
310 320 330 340 350
SLWDKSGHLE KFGDDIFSLP LEPQQYVIKP MSCPAHVQIF NQGVKSYRDL
360 370 380 390 400
PLRYAEFGAC HRNEPSGTLH GLMRLRGFVQ DDAHIFCTED QIQSEVSAFI
410 420 430 440 450
DQLHEVYADF GFTEVIHKLS TRPEKRVGSD EVWTKAEQAL AEALNRKGVE
460 470 480 490 500
WDILPGEGAF YGPKIEFSLR DCLGRIWQCG TVQVDFSVPE RLGAHYIAED
510 520 530 540 550
GSKKPPVMIH RAILGSFERF LGILLEESAG KLPLWLAPVQ VVVMNITDRQ
560 570 580 590 600
ADYVGQTVEN LQNLGIRAHS DLRNEKIGFK IREHTIARVP YLVVIGDREV
610 620 630 640 650
ADKTLSVRAL EDEASTTITL EEFARQLKAE ISQRSRKSPA PSPLFPVGGE

S
Length:651
Mass (Da):73,784
Last modified:June 1, 2003 - v1
Checksum:i8BADE57CA2D780D2
GO

Sequence cautioni

The sequence AAO90830 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90830.2. Different initiation.
RefSeqiNP_820316.2. NC_002971.3.

Genome annotation databases

EnsemblBacteriaiAAO90830; AAO90830; CBU_1326.
GeneIDi1209232.
KEGGicbu:CBU_1326.
PATRICifig|227377.7.peg.1317.

Similar proteinsi

Entry informationi

Entry nameiSYT_COXBU
AccessioniPrimary (citable) accession number: Q83C10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2003
Last modified: November 22, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families