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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of GMP from XMP.

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (guaA)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Nucleophile1
Active sitei1811
Active sitei1831

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi235 – 241ATP7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processGMP biosynthesis, Purine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:guaA
Ordered Locus Names:CBU_1341
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001401201 – 524GMP synthase [glutamine-hydrolyzing]Add BLAST524

Proteomic databases

PRIDEiQ83BZ6.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi227377.CBU_1341.

Structurei

Secondary structure

1524
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 14Combined sources7
Helixi20 – 30Combined sources11
Beta strandi33 – 38Combined sources6
Beta strandi43 – 46Combined sources4
Turni47 – 49Combined sources3
Beta strandi52 – 56Combined sources5
Turni75 – 77Combined sources3
Beta strandi78 – 80Combined sources3
Beta strandi82 – 85Combined sources4
Helixi87 – 95Combined sources9
Beta strandi96 – 98Combined sources3
Beta strandi108 – 117Combined sources10
Turni119 – 122Combined sources4
Beta strandi136 – 145Combined sources10
Beta strandi155 – 160Combined sources6
Beta strandi163 – 169Combined sources7
Beta strandi171 – 173Combined sources3
Beta strandi175 – 180Combined sources6
Beta strandi182 – 184Combined sources3
Helixi190 – 199Combined sources10
Helixi211 – 225Combined sources15
Beta strandi230 – 233Combined sources4
Turni235 – 237Combined sources3
Helixi238 – 251Combined sources14
Helixi252 – 254Combined sources3
Beta strandi255 – 260Combined sources6
Helixi269 – 277Combined sources9
Beta strandi284 – 288Combined sources5
Helixi291 – 295Combined sources5
Beta strandi296 – 300Combined sources5
Helixi304 – 321Combined sources18
Turni322 – 327Combined sources6
Beta strandi330 – 332Combined sources3
Helixi337 – 341Combined sources5
Turni374 – 377Combined sources4
Helixi380 – 390Combined sources11
Helixi394 – 397Combined sources4
Helixi406 – 408Combined sources3
Beta strandi409 – 411Combined sources3
Helixi418 – 435Combined sources18
Turni436 – 438Combined sources3
Helixi440 – 442Combined sources3
Beta strandi443 – 455Combined sources13
Beta strandi466 – 474Combined sources9
Helixi489 – 502Combined sources14
Beta strandi503 – 505Combined sources3
Beta strandi509 – 512Combined sources4
Turni516 – 518Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TQIX-ray2.84A/B/C/D1-524[»]
ProteinModelPortaliQ83BZ6.
SMRiQ83BZ6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ83BZ6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 207Glutamine amidotransferase type-1Add BLAST199
Domaini208 – 399GMPS ATP-PPaseAdd BLAST192

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CM0. Bacteria.
COG0518. LUCA.
COG0519. LUCA.
HOGENOMiHOG000223964.
KOiK01951.
OMAiKRKIIGH.

Family and domain databases

CDDicd01742. GATase1_GMP_Synthase. 1 hit.
cd01997. GMP_synthase_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_GATase.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83BZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKDIHQHRI LILDFGSQYA QLIARRVREI GVYCELMPCD IDEETIRDFN
60 70 80 90 100
PHGIILSGGP ETVTLSHTLR APAFIFEIGC PVLGICYGMQ TMAYQLGGKV
110 120 130 140 150
NRTAKAEFGH AQLRVLNPAF LFDGIEDQVS PQGEPLLDVW MSHGDIVSEL
160 170 180 190 200
PPGFEATACT DNSPLAAMAD FKRRFFGLQF HPEVTHTPQG HRILAHFVIH
210 220 230 240 250
ICQCIPNWTT KHIIEDSIRD IQEKVGKEQV IVGLSGGVDS AVTATLVHKA
260 270 280 290 300
IGDQLVCVLV DTGLLRLNEV DEVLNVFQKH LGAKVICVDA KDRFMKALKG
310 320 330 340 350
ISDPEEKRKI AGEQFIRVFE EQAKKLNVKW LGQGTIYPDV IESAKTKTGK
360 370 380 390 400
GHIIKTHHNV GGLPLNMELK LIEPLRELFK DEVRKLGLEL GLPADLIYRH
410 420 430 440 450
PFPGPGLAIR ILGEVSAEYI NILKQADAIF IEELKKSDYY HQVSQAFAVF
460 470 480 490 500
MPLKSVGVKG DARHYGYIIA LRAVKTVDFM TAQWADLPHE FLSKVSHRIV
510 520
NEIKEVSRVV YDMTNKPPAT IEWE
Length:524
Mass (Da):58,682
Last modified:June 1, 2003 - v1
Checksum:iF45A43498D93317D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90844.1.
RefSeqiNP_820330.1. NC_002971.3.
WP_010958163.1. NC_002971.4.

Genome annotation databases

EnsemblBacteriaiAAO90844; AAO90844; CBU_1341.
GeneIDi1209247.
KEGGicbu:CBU_1341.
PATRICifig|227377.7.peg.1333.

Similar proteinsi

Entry informationi

Entry nameiGUAA_COXBU
AccessioniPrimary (citable) accession number: Q83BZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2003
Last modified: June 7, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references