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Q83BY4 (RLME_COXBU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase E

EC=2.1.1.166
Alternative name(s):
23S rRNA Um2552 methyltransferase
rRNA (uridine-2'-O-)-methyltransferase
Gene names
Name:rlmE
Synonyms:ftsJ, rrmJ
Ordered Locus Names:CBU_1353
OrganismCoxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]
Taxonomic identifier227377 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit By similarity. HAMAP-Rule MF_01547

Catalytic activity

S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA. HAMAP-Rule MF_01547

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01547.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmE family.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionrRNA (uridine-2'-O-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 212212Ribosomal RNA large subunit methyltransferase E HAMAP-Rule MF_01547
PRO_0000155489

Sites

Active site1621Proton acceptor By similarity
Binding site571S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site591S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site771S-adenosyl-L-methionine By similarity
Binding site931S-adenosyl-L-methionine By similarity
Binding site1221S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q83BY4 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: D5E1DD704C8FE8B7

FASTA21223,862
        10         20         30         40         50         60 
MTHSKRWLEE HEKDPYVKRA KKEGYPSRAA YKLLEIHQKY KLFKPSMNVI DLGAAPGGWS 

        70         80         90        100        110        120 
QVAKDLVGPK GVVIAIDLLP MQSMLDVIFI QGDFNEPEIF NQLEAIVAKK TLTGQVDLVI 

       130        140        150        160        170        180 
SDMAPNISGI KNVDQSRSLH LVELAWDCAQ KLLARGGTFL VKVFQGPGVD RFLINLRPYF 

       190        200        210 
NQVKFLKPSA SRSRSSEIYI LAGEFLGYNQ RV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016828 Genomic DNA. Translation: AAO90856.1.
RefSeqNP_820342.1. NC_002971.3.

3D structure databases

ProteinModelPortalQ83BY4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING227377.CBU_1353.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO90856; AAO90856; CBU_1353.
GeneID1209259.
KEGGcbu:CBU_1353.
PATRIC17931449. VBICoxBur82552_1348.

Phylogenomic databases

eggNOGCOG0293.
HOGENOMHOG000162366.
KOK02427.
OMAQYVVSQI.
OrthoDBEOG657JC0.
ProtClustDBCLSK914723.

Enzyme and pathway databases

BioCycCBUR227377:GJ7S-1343-MONOMER.

Family and domain databases

HAMAPMF_01547. RNA_methyltr_E.
InterProIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
[Graphical view]
PANTHERPTHR10920. PTHR10920. 1 hit.
PfamPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFPIRSF005461. 23S_rRNA_mtase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRLME_COXBU
AccessionPrimary (citable) accession number: Q83BY4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Coxiella burnetii

Coxiella burnetii (strain RSA 493): entries and gene names