Q83BY4 (RLME_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase E
EC=2.1.1.166

Alternative name(s):
23S rRNA Um2552 methyltransferase
rRNA (uridine-2'-O-)-methyltransferase

Gene names

Name:	rlmE
Synonyms:	ftsJ, rrmJ
Ordered Locus Names:	CBU_1353

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit By similarity. HAMAP-Rule MF_01547
Catalytic activity	S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA. HAMAP-Rule MF_01547
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01547.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmE family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	rRNA (uridine-2'-O-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Ribosomal RNA large subunit methyltransferase E HAMAP-Rule MF_01547		PRO_0000155489
Sites
	Active site	162	1	Proton acceptor By similarity
	Binding site	57	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	59	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	77	1	S-adenosyl-L-methionine By similarity
	Binding site	93	1	S-adenosyl-L-methionine By similarity
	Binding site	122	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83BY4 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D5E1DD704C8FE8B7	FASTA	212	23,862
	10 20 30 40 50 60 MTHSKRWLEE HEKDPYVKRA KKEGYPSRAA YKLLEIHQKY KLFKPSMNVI DLGAAPGGWS 70 80 90 100 110 120 QVAKDLVGPK GVVIAIDLLP MQSMLDVIFI QGDFNEPEIF NQLEAIVAKK TLTGQVDLVI 130 140 150 160 170 180 SDMAPNISGI KNVDQSRSLH LVELAWDCAQ KLLARGGTFL VKVFQGPGVD RFLINLRPYF 190 200 210 NQVKFLKPSA SRSRSSEIYI LAGEFLGYNQ RV

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO90856.1.
RefSeq	NP_820342.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83BY4.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1353.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO90856; AAO90856; CBU_1353.
GeneID	1209259.
KEGG	cbu:CBU_1353.
PATRIC	17931449. VBICoxBur82552_1348.
Phylogenomic databases
eggNOG	COG0293.
HOGENOM	HOG000162366.
KO	K02427.
OMA	NDPYVQR.
ProtClustDB	CLSK914723.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1343-MONOMER.
Family and domain databases
HAMAP	MF_01547. RNA_methyltr_E.
InterPro	IPR015507. rRNA-MeTfrase_E. IPR002877. rRNA_MeTrfase_FtsJ_dom. [Graphical view]
PANTHER	PTHR10920. PTHR10920. 1 hit.
Pfam	PF01728. FtsJ. 1 hit. [Graphical view]
PIRSF	PIRSF005461. 23S_rRNA_mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLME_COXBU

Accession

Primary (citable) accession number: Q83BY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program