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Protein

Ribosomal RNA large subunit methyltransferase E

Gene

rlmE

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyluridine(2552) in 23S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei59 – 591S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei77 – 771S-adenosyl-L-methionineUniRule annotation
Binding sitei93 – 931S-adenosyl-L-methionineUniRule annotation
Binding sitei122 – 1221S-adenosyl-L-methionineUniRule annotation
Active sitei162 – 1621Proton acceptorUniRule annotation

GO - Molecular functioni

  1. rRNA (uridine-2'-O-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCBUR227377:GJ7S-1343-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase EUniRule annotation (EC:2.1.1.166UniRule annotation)
Alternative name(s):
23S rRNA Um2552 methyltransferaseUniRule annotation
rRNA (uridine-2'-O-)-methyltransferaseUniRule annotation
Gene namesi
Name:rlmEUniRule annotation
Synonyms:ftsJUniRule annotation, rrmJUniRule annotation
Ordered Locus Names:CBU_1353
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000002671: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212Ribosomal RNA large subunit methyltransferase EPRO_0000155489Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi227377.CBU_1353.

Structurei

3D structure databases

ProteinModelPortaliQ83BY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0293.
HOGENOMiHOG000162366.
KOiK02427.
OMAiQYVVSQI.
OrthoDBiEOG657JC0.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 1 hit.
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF005461. 23S_rRNA_mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83BY4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTHSKRWLEE HEKDPYVKRA KKEGYPSRAA YKLLEIHQKY KLFKPSMNVI
60 70 80 90 100
DLGAAPGGWS QVAKDLVGPK GVVIAIDLLP MQSMLDVIFI QGDFNEPEIF
110 120 130 140 150
NQLEAIVAKK TLTGQVDLVI SDMAPNISGI KNVDQSRSLH LVELAWDCAQ
160 170 180 190 200
KLLARGGTFL VKVFQGPGVD RFLINLRPYF NQVKFLKPSA SRSRSSEIYI
210
LAGEFLGYNQ RV
Length:212
Mass (Da):23,862
Last modified:June 1, 2003 - v1
Checksum:iD5E1DD704C8FE8B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90856.1.
RefSeqiNP_820342.1. NC_002971.3.

Genome annotation databases

EnsemblBacteriaiAAO90856; AAO90856; CBU_1353.
GeneIDi1209259.
KEGGicbu:CBU_1353.
PATRICi17931449. VBICoxBur82552_1348.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO90856.1.
RefSeqiNP_820342.1. NC_002971.3.

3D structure databases

ProteinModelPortaliQ83BY4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi227377.CBU_1353.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO90856; AAO90856; CBU_1353.
GeneIDi1209259.
KEGGicbu:CBU_1353.
PATRICi17931449. VBICoxBur82552_1348.

Phylogenomic databases

eggNOGiCOG0293.
HOGENOMiHOG000162366.
KOiK02427.
OMAiQYVVSQI.
OrthoDBiEOG657JC0.

Enzyme and pathway databases

BioCyciCBUR227377:GJ7S-1343-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01547. RNA_methyltr_E.
InterProiIPR015507. rRNA-MeTfrase_E.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR10920. PTHR10920. 1 hit.
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
PIRSFiPIRSF005461. 23S_rRNA_mtase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RSA 493 / Nine Mile phase I.

Entry informationi

Entry nameiRLME_COXBU
AccessioniPrimary (citable) accession number: Q83BY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.