Q83BE2 (RUVA_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvA
EC=3.6.4.12

Gene names

Name:	ruvA
Ordered Locus Names:	CBU_1568

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB By similarity. HAMAP-Rule MF_00031
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00031
Subunit structure	Forms a complex with RuvB By similarity. HAMAP-Rule MF_00031
Sequence similarities	Belongs to the RuvA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Holliday junction helicase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW four-way junction helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Holliday junction ATP-dependent DNA helicase RuvA HAMAP-Rule MF_00031		PRO_0000094627

Sequences

	Sequence		Length	Mass (Da)
	Q83BE2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1C48A1F35D1CC2DE	FASTA	200	22,225
	10 20 30 40 50 60 MIGHLRGIIV EKQPPYLLLE VAGVGYEITA PLSTFYHLPE PQEEILLYTH LIVREDAHTL 70 80 90 100 110 120 YGFHNDHERR LFRALIKVNG VGPKLALAIL SGIGPDEFVH CVLNQNIDQL VRIPGVGRKT 130 140 150 160 170 180 AERLVIETKD GLSRWHTNDT PSPEGLRSSN TQPTQDAISA LMALGYKPQE AKRAIDAIQK 190 200 PDLSAETLIR LALKQMVLGT

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO91065.1.
RefSeq	NP_820551.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83BE2.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1568.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO91065; AAO91065; CBU_1568.
GeneID	1209478.
KEGG	cbu:CBU_1568.
PATRIC	17931899. VBICoxBur82552_1569.
Phylogenomic databases
eggNOG	COG0632.
HOGENOM	HOG000057116.
KO	K03550.
OMA	ALEREWF.
ProtClustDB	PRK00116.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1547-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_00031. DNA_helic_RuvA.
InterPro	IPR011114. DNA_helicas_Holl-junc_RuvA_C. IPR013849. DNA_helicase_Holl-junc_RuvA_I. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR000085. RuvA. IPR010994. RuvA_2-like. [Graphical view]
Pfam	PF07499. RuvA_C. 1 hit. PF01330. RuvA_N. 1 hit. [Graphical view]
SMART	SM00278. HhH1. 2 hits. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47781. RuvA_2_like. 1 hit. SSF46929. RuvA_C-like. 1 hit.
TIGRFAMs	TIGR00084. ruvA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVA_COXBU

Accession

Primary (citable) accession number: Q83BE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2003
Last modified:	May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program