Q83BE0 (RUVB_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvB
EC=3.6.4.12

Gene names

Name:	ruvB
Ordered Locus Names:	CBU_1570

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing By similarity. HAMAP-Rule MF_00016
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00016
Subunit structure	Forms a complex with RuvA By similarity. HAMAP-Rule MF_00016
Sequence similarities	Belongs to the RuvB family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Holliday junction ATP-dependent DNA helicase RuvB HAMAP-Rule MF_00016		PRO_0000165525
Regions
	Nucleotide binding	64 – 71	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q83BE0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7A69DB0B71CC72FF	FASTA	351	39,151
	10 20 30 40 50 60 MDEKIETRLI GATVQSNEPD LERSIRPKRL SDYIGQAAVR EQMDIFIRAA TNRREALDHV 70 80 90 100 110 120 LIFGPPGLGK TTLAHIIAHE MQAGLKQTSG PVLEKAGDLA ALLTNLEPHD VLFIDEIHRL 130 140 150 160 170 180 NPAIEEVLYP ALEDFQLDII IGEGPSARSI KLDLPPFTLV GATTRAGLLT SPLRDRFGIV 190 200 210 220 230 240 QRLEFYRIPD LIHIVKRAAH ILNVVIDENG AGEIARRSRG TPRIANRLLR RVRDFAEVRA 250 260 270 280 290 300 NGMINESIAK EALDLLNVDI RGLDVMDRKL LETIIKKFQG GPVGIESLAA AISEERGTIE 310 320 330 340 350 DVIEPYLIQE GFILRTPRGR IATELTYQHF KLPLPTQSTL QENFDLLGKV E

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO91067.1.
RefSeq	NP_820553.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83BE0.
SMR	Q83BE0. Positions 24-336.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1570.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO91067; AAO91067; CBU_1570.
GeneID	1209480.
KEGG	cbu:CBU_1570.
PATRIC	17931903. VBICoxBur82552_1571.
Phylogenomic databases
eggNOG	COG2255.
HOGENOM	HOG000218623.
KO	K03551.
OMA	HIIAKEM.
ProtClustDB	PRK00080.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1549-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
HAMAP	MF_00016. DNA_helic_RuvB.
InterPro	IPR003593. AAA+_ATPase. IPR004605. DNA_helicase_Holl-junc_RuvB. IPR008823. DNA_helicase_Holl-junc_RuvB_C. IPR008824. DNA_helicase_Holl-junc_RuvB_N. IPR027417. P-loop_NTPase. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR13779:SF2. PTHR13779:SF2. 1 hit.
Pfam	PF05491. RuvB_C. 1 hit. PF05496. RuvB_N. 1 hit. [Graphical view]
ProDom	PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00635. ruvB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVB_COXBU

Accession

Primary (citable) accession number: Q83BE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2003
Last modified:	May 29, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program