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Protein

Bifunctional protein HldE

Gene

hldE

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.UniRule annotation
Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.UniRule annotation

Catalytic activityi

D-glycero-beta-D-manno-heptose 7-phosphate + ATP = D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP.UniRule annotation
D-glycero-beta-D-manno-heptose 1-phosphate + ATP = ADP-D-glycero-beta-D-manno-heptose + diphosphate.UniRule annotation

Pathwayi: ADP-L-glycero-beta-D-manno-heptose biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional protein HldE (hldE), Bifunctional protein HldE (CBU_0678)
  2. no protein annotated in this organism
  3. Bifunctional protein HldE (hldE), Bifunctional protein HldE (CBU_0678)
  4. no protein annotated in this organism
This subpathway is part of the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate, the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei266UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 200ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Nucleotidyltransferase, Transferase
Biological processCarbohydrate metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00356; UER00437.
UPA00356; UER00439.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein HldEUniRule annotation
Including the following 2 domains:
D-beta-D-heptose 7-phosphate kinaseUniRule annotation (EC:2.7.1.167UniRule annotation)
Alternative name(s):
D-beta-D-heptose 7-phosphotransferaseUniRule annotation
D-glycero-beta-D-manno-heptose-7-phosphate kinaseUniRule annotation
D-beta-D-heptose 1-phosphate adenylyltransferaseUniRule annotation (EC:2.7.7.70UniRule annotation)
Alternative name(s):
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferaseUniRule annotation
Gene namesi
Name:hldEUniRule annotation
Synonyms:rfaE
Ordered Locus Names:CBU_1655
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000801061 – 475Bifunctional protein HldEAdd BLAST475

Proteomic databases

PRIDEiQ83B60.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi227377.CBU_1655.

Structurei

3D structure databases

ProteinModelPortaliQ83B60.
SMRiQ83B60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 321RibokinaseAdd BLAST321
Regioni346 – 475CytidylyltransferaseAdd BLAST130

Sequence similaritiesi

In the N-terminal section; belongs to the carbohydrate kinase PfkB family.UniRule annotation
In the C-terminal section; belongs to the cytidylyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DW0. Bacteria.
COG2870. LUCA.
HOGENOMiHOG000237584.
KOiK03272.
OMAiCDPKGKD.

Family and domain databases

CDDicd01172. RfaE_like. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01603. HldE. 1 hit.
InterProiView protein in InterPro
IPR023030. Bifunc_HldE.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF01467. CTP_transf_like. 1 hit.
PF00294. PfkB. 1 hit.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.
PROSITEiView protein in PROSITE
PS00583. PFKB_KINASES_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83B60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKIDISLY EKARIVVYGD IMLDRYWYGQ ASRISPEAPI PVVNVDQIEA
60 70 80 90 100
RPGGAANVAL NVAALGASVE LMGVVGDDQE SRELETLLNK KSINCHFHRL
110 120 130 140 150
NDHPTVTKLR VLGQNQQLLR LDFEKTLKHY EDKGLLQRYQ HSLSHAQAVI
160 170 180 190 200
LSDYAKGALF NVTAPIQRAR EKGLPVLVDP KSVDFSRYAG ATLLTPNLKE
210 220 230 240 250
FEAVVGHCRT DQELEFKARA LIHQYRFEAI LITRGKQGMM LIQKEGAAIN
260 270 280 290 300
LVAHAREVYD VTGAGDTVIA VMAASLAAGG DFYEAAQLAN LAAGLVVRKL
310 320 330 340 350
GAATVTVPEL RRALHQITAS HHGILSEKAL LLAVADARAH GETIVMTNGC
360 370 380 390 400
FDILHAGHVH YLEAAKAMGH RLIVAVNDDN SVRRLKGKDR PINSLQARME
410 420 430 440 450
VLTALRAIDW VVPFSEDTPA RLITEVLPNI LVKGGDYQPS QIAGGDEVVK
460 470
NGGKVLTIPI KEGFSTSRLV EKMLN
Length:475
Mass (Da):51,922
Last modified:June 1, 2003 - v1
Checksum:i1C4AC52C4A818130
GO

Sequence cautioni

Q83B60: The sequence AAO91151 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO91151.2. Different initiation.
RefSeqiNP_820637.2. NC_002971.3.

Genome annotation databases

EnsemblBacteriaiAAO91151; AAO91151; CBU_1655.
GeneIDi1209566.
KEGGicbu:CBU_1655.
PATRICifig|227377.7.peg.1646.

Similar proteinsi

Entry informationi

Entry nameiHLDE_COXBU
AccessioniPrimary (citable) accession number: Q83B60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2003
Last modified: July 5, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families