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Q83B44

- ENO_COXBU

UniProt

Q83B44 - ENO_COXBU

Protein

Enolase

Gene

eno

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

    Catalytic activityi

    2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei157 – 1571SubstrateUniRule annotation
    Binding sitei166 – 1661SubstrateUniRule annotation
    Active sitei207 – 2071Proton donorUniRule annotation
    Metal bindingi244 – 2441MagnesiumUniRule annotation
    Metal bindingi285 – 2851MagnesiumUniRule annotation
    Binding sitei285 – 2851SubstrateUniRule annotation
    Metal bindingi312 – 3121MagnesiumUniRule annotation
    Binding sitei312 – 3121SubstrateUniRule annotation
    Active sitei337 – 3371Proton acceptorUniRule annotation
    Binding sitei337 – 3371Substrate (covalent); in inhibited formUniRule annotation
    Binding sitei388 – 3881SubstrateUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. phosphopyruvate hydratase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciCBUR227377:GJ7S-1645-MONOMER.
    UniPathwayiUPA00109; UER00187.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
    Alternative name(s):
    2-phospho-D-glycerate hydro-lyaseUniRule annotation
    2-phosphoglycerate dehydrataseUniRule annotation
    Gene namesi
    Name:enoUniRule annotation
    Ordered Locus Names:CBU_1674
    OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
    Taxonomic identifieri227377 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
    ProteomesiUP000002671: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation. Secreted UniRule annotation. Cell surface UniRule annotation
    Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

    GO - Cellular componenti

    1. cell surface Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-SubCell
    3. phosphopyruvate hydratase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428EnolasePRO_0000133877Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

    Protein-protein interaction databases

    STRINGi227377.CBU_1674.

    Structurei

    Secondary structure

    1
    428
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 1410
    Beta strandi20 – 289
    Beta strandi33 – 375
    Helixi60 – 623
    Helixi66 – 738
    Helixi75 – 806
    Helixi88 – 9912
    Turni105 – 1073
    Helixi109 – 12618
    Helixi131 – 1366
    Turni137 – 1393
    Beta strandi146 – 1538
    Beta strandi163 – 1708
    Helixi177 – 19721
    Helixi217 – 23014
    Turni235 – 2373
    Beta strandi240 – 2445
    Helixi247 – 2493
    Beta strandi250 – 2523
    Beta strandi259 – 2613
    Helixi265 – 27814
    Beta strandi281 – 2855
    Helixi293 – 30311
    Turni304 – 3063
    Beta strandi307 – 3126
    Turni313 – 3175
    Helixi319 – 3279
    Beta strandi332 – 3365
    Helixi338 – 3414
    Helixi344 – 35613
    Beta strandi360 – 3645
    Helixi374 – 3818
    Beta strandi385 – 3884
    Beta strandi392 – 3943
    Helixi395 – 41117
    Helixi419 – 4224

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TQPX-ray2.20A/B1-428[»]
    ProteinModelPortaliQ83B44.
    SMRiQ83B44. Positions 3-423.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni364 – 3674Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the enolase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0148.
    HOGENOMiHOG000072174.
    KOiK01689.
    OMAiRSEIKGQ.
    OrthoDBiEOG65J589.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPiMF_00318. Enolase.
    InterProiIPR000941. Enolase.
    IPR020810. Enolase_C.
    IPR029065. Enolase_C-like.
    IPR020809. Enolase_CS.
    IPR020811. Enolase_N.
    IPR029017. Enolase_N_like.
    [Graphical view]
    PANTHERiPTHR11902. PTHR11902. 1 hit.
    PfamiPF00113. Enolase_C. 1 hit.
    PF03952. Enolase_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001400. Enolase. 1 hit.
    PRINTSiPR00148. ENOLASE.
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR01060. eno. 1 hit.
    PROSITEiPS00164. ENOLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q83B44-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTATITDINA HEILDSRANP TLEVRVTLSS QAYGCAAVPS GASTGEREAV    50
    ELRDNDLERY GGKGVLQAVE NVNGPIRDAL LGQDPRSQEE IDRIMIELDG 100
    TENKANLGAN AILGVSLAVA YAAANNADLP LYRYLGGDGG PFSMPVPMMN 150
    IINGGAHATN NLDFQEFMIV PVGAPTFAEA LRYGAEVFHA LKKRLVSRGL 200
    MSAVGDEGGF APDLPNNEAA FELILEAIED ANYVPGKDIY LALDAASSEL 250
    YQNGRYDFEN NQLTSEEMID RLTEWTKKYP VISIEDGLSE NDWAGWKLLT 300
    ERLENKVQLV GDDIFVTNPD ILEKGIKKNI ANAILVKLNQ IGTLTETLAT 350
    VGLAKSNKYG VIISHRSGET EDTTIADLAV ATDARQIKTG SLCRSDRVAK 400
    YNRLLQIERE LNDQAPYAGK EAFLFNRK 428
    Length:428
    Mass (Da):46,626
    Last modified:June 1, 2003 - v1
    Checksum:iB58295D02CC2BE61
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO91170.1.
    RefSeqiNP_820656.1. NC_002971.3.

    Genome annotation databases

    EnsemblBacteriaiAAO91170; AAO91170; CBU_1674.
    GeneIDi1209585.
    KEGGicbu:CBU_1674.
    PATRICi17932089. VBICoxBur82552_1663.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016828 Genomic DNA. Translation: AAO91170.1 .
    RefSeqi NP_820656.1. NC_002971.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TQP X-ray 2.20 A/B 1-428 [» ]
    ProteinModelPortali Q83B44.
    SMRi Q83B44. Positions 3-423.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 227377.CBU_1674.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO91170 ; AAO91170 ; CBU_1674 .
    GeneIDi 1209585.
    KEGGi cbu:CBU_1674.
    PATRICi 17932089. VBICoxBur82552_1663.

    Phylogenomic databases

    eggNOGi COG0148.
    HOGENOMi HOG000072174.
    KOi K01689.
    OMAi RSEIKGQ.
    OrthoDBi EOG65J589.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00187 .
    BioCyci CBUR227377:GJ7S-1645-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPi MF_00318. Enolase.
    InterProi IPR000941. Enolase.
    IPR020810. Enolase_C.
    IPR029065. Enolase_C-like.
    IPR020809. Enolase_CS.
    IPR020811. Enolase_N.
    IPR029017. Enolase_N_like.
    [Graphical view ]
    PANTHERi PTHR11902. PTHR11902. 1 hit.
    Pfami PF00113. Enolase_C. 1 hit.
    PF03952. Enolase_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001400. Enolase. 1 hit.
    PRINTSi PR00148. ENOLASE.
    SUPFAMi SSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsi TIGR01060. eno. 1 hit.
    PROSITEi PS00164. ENOLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RSA 493 / Nine Mile phase I.

    Entry informationi

    Entry nameiENO_COXBU
    AccessioniPrimary (citable) accession number: Q83B44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 15, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Coxiella burnetii
      Coxiella burnetii (strain RSA 493): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3