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Q83B44

- ENO_COXBU

UniProt

Q83B44 - ENO_COXBU

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Protein
Enolase
Gene
eno, CBU_1674
Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis By similarity.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei157 – 1571Substrate By similarity
Binding sitei166 – 1661Substrate By similarity
Active sitei207 – 2071Proton donor By similarity
Metal bindingi244 – 2441Magnesium By similarity
Metal bindingi285 – 2851Magnesium By similarity
Binding sitei285 – 2851Substrate By similarity
Metal bindingi312 – 3121Magnesium By similarity
Binding sitei312 – 3121Substrate By similarity
Active sitei337 – 3371Proton acceptor By similarity
Binding sitei337 – 3371Substrate (covalent); in inhibited form By similarity
Binding sitei388 – 3881Substrate By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. phosphopyruvate hydratase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCBUR227377:GJ7S-1645-MONOMER.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:eno
Ordered Locus Names:CBU_1674
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
ProteomesiUP000002671: Chromosome

Subcellular locationi

Cytoplasm By similarity. Secreted By similarity. Cell surface By similarity
Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface By similarity.UniRule annotation

GO - Cellular componenti

  1. cell surface Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-SubCell
  3. phosphopyruvate hydratase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428EnolaseUniRule annotation
PRO_0000133877Add
BLAST

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Protein-protein interaction databases

STRINGi227377.CBU_1674.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 1410
Beta strandi20 – 289
Beta strandi33 – 375
Helixi60 – 623
Helixi66 – 738
Helixi75 – 806
Helixi88 – 9912
Turni105 – 1073
Helixi109 – 12618
Helixi131 – 1366
Turni137 – 1393
Beta strandi146 – 1538
Beta strandi163 – 1708
Helixi177 – 19721
Helixi217 – 23014
Turni235 – 2373
Beta strandi240 – 2445
Helixi247 – 2493
Beta strandi250 – 2523
Beta strandi259 – 2613
Helixi265 – 27814
Beta strandi281 – 2855
Helixi293 – 30311
Turni304 – 3063
Beta strandi307 – 3126
Turni313 – 3175
Helixi319 – 3279
Beta strandi332 – 3365
Helixi338 – 3414
Helixi344 – 35613
Beta strandi360 – 3645
Helixi374 – 3818
Beta strandi385 – 3884
Beta strandi392 – 3943
Helixi395 – 41117
Helixi419 – 4224

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TQPX-ray2.20A/B1-428[»]
ProteinModelPortaliQ83B44.
SMRiQ83B44. Positions 3-423.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni364 – 3674Substrate binding By similarity

Sequence similaritiesi

Belongs to the enolase family.

Phylogenomic databases

eggNOGiCOG0148.
HOGENOMiHOG000072174.
KOiK01689.
OMAiRSEIKGQ.
OrthoDBiEOG65J589.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N_like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q83B44-1 [UniParc]FASTAAdd to Basket

« Hide

MTATITDINA HEILDSRANP TLEVRVTLSS QAYGCAAVPS GASTGEREAV    50
ELRDNDLERY GGKGVLQAVE NVNGPIRDAL LGQDPRSQEE IDRIMIELDG 100
TENKANLGAN AILGVSLAVA YAAANNADLP LYRYLGGDGG PFSMPVPMMN 150
IINGGAHATN NLDFQEFMIV PVGAPTFAEA LRYGAEVFHA LKKRLVSRGL 200
MSAVGDEGGF APDLPNNEAA FELILEAIED ANYVPGKDIY LALDAASSEL 250
YQNGRYDFEN NQLTSEEMID RLTEWTKKYP VISIEDGLSE NDWAGWKLLT 300
ERLENKVQLV GDDIFVTNPD ILEKGIKKNI ANAILVKLNQ IGTLTETLAT 350
VGLAKSNKYG VIISHRSGET EDTTIADLAV ATDARQIKTG SLCRSDRVAK 400
YNRLLQIERE LNDQAPYAGK EAFLFNRK 428
Length:428
Mass (Da):46,626
Last modified:June 1, 2003 - v1
Checksum:iB58295D02CC2BE61
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016828 Genomic DNA. Translation: AAO91170.1.
RefSeqiNP_820656.1. NC_002971.3.

Genome annotation databases

EnsemblBacteriaiAAO91170; AAO91170; CBU_1674.
GeneIDi1209585.
KEGGicbu:CBU_1674.
PATRICi17932089. VBICoxBur82552_1663.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016828 Genomic DNA. Translation: AAO91170.1 .
RefSeqi NP_820656.1. NC_002971.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3TQP X-ray 2.20 A/B 1-428 [» ]
ProteinModelPortali Q83B44.
SMRi Q83B44. Positions 3-423.
ModBasei Search...

Protein-protein interaction databases

STRINGi 227377.CBU_1674.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO91170 ; AAO91170 ; CBU_1674 .
GeneIDi 1209585.
KEGGi cbu:CBU_1674.
PATRICi 17932089. VBICoxBur82552_1663.

Phylogenomic databases

eggNOGi COG0148.
HOGENOMi HOG000072174.
KOi K01689.
OMAi RSEIKGQ.
OrthoDBi EOG65J589.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00187 .
BioCyci CBUR227377:GJ7S-1645-MONOMER.

Family and domain databases

Gene3Di 3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPi MF_00318. Enolase.
InterProi IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N_like.
[Graphical view ]
PANTHERi PTHR11902. PTHR11902. 1 hit.
Pfami PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF001400. Enolase. 1 hit.
PRINTSi PR00148. ENOLASE.
SUPFAMi SSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsi TIGR01060. eno. 1 hit.
PROSITEi PS00164. ENOLASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RSA 493 / Nine Mile phase I.

Entry informationi

Entry nameiENO_COXBU
AccessioniPrimary (citable) accession number: Q83B44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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