Reviewed, UniProtKB/Swiss-Prot Q83B43 (KDSA_COXBU)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    KDO-8-phosphate synthetase
      Short name=KDO 8-P synthase
      Short name=KDOPS

Gene names

Name:	kdsA
Ordered Locus Names:	CBU_1675

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the kdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000187121

Sequences

	Sequence		Length	Mass (Da)
	Q83B43-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 56B00DBDEAC19389	FASTA	280	30,507
	10 20 30 40 50 60 MLMQIADFEI GLNNPLFLIA GPCVIESEAL VMDVAGELKS ITQQLDMPFI FKASFDKANR 70 80 90 100 110 120 SSHLSYRGPG IEKGLTILEK VKKTLEVPII TDVHEDTPLQ EVAAVVDVLQ TPAFLCRQSN 130 140 150 160 170 180 FIRSVAACGK PVNIKKGQFL APWEMKQVVA KAWATGNKKI MVCERGYSFG YNNLISDMRA 190 200 210 220 230 240 LAILRETACP VIFDATHSVQ LPGGHGTNSG GQREFVPVLA RAATAAGIAG IFMETHPDPD 250 260 270 280 RALSDGPNSW PLAKMQPLLE TLKELDKVVK NAGFLEQSSE

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO91171.1.
RefSeq	NP_820657.1.
3D structure databases
HSSP	HSSP built from PDB template 1D9E based on UniProtKB P17579.
ModBase	Search...
Genome annotation databases
GeneID	1209586.
GenomeReviews	Gene locus CBU_1675 in contig AE016828_GR.
KEGG	cbu:CBU_1675.
NMPDR	fig|227377.1.peg.1601.
TIGR	CBU_1675.
Phylogenomic databases
HOGENOM	Q83B43.
OMA	VYKTSFD.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_1675-MON.
BRENDA	2.5.1.55. 256353.
Family and domain databases
HAMAP	MF_00056. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_COXBU

Accession

Primary (citable) accession number: Q83B43

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)