Q83B43 (KDSA_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	CBU_1675

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP-Rule MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP-Rule MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP-Rule MF_00056
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00056.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP-Rule MF_00056		PRO_0000187121

Sequences

	Sequence		Length	Mass (Da)
	Q83B43 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 56B00DBDEAC19389	FASTA	280	30,507
	10 20 30 40 50 60 MLMQIADFEI GLNNPLFLIA GPCVIESEAL VMDVAGELKS ITQQLDMPFI FKASFDKANR 70 80 90 100 110 120 SSHLSYRGPG IEKGLTILEK VKKTLEVPII TDVHEDTPLQ EVAAVVDVLQ TPAFLCRQSN 130 140 150 160 170 180 FIRSVAACGK PVNIKKGQFL APWEMKQVVA KAWATGNKKI MVCERGYSFG YNNLISDMRA 190 200 210 220 230 240 LAILRETACP VIFDATHSVQ LPGGHGTNSG GQREFVPVLA RAATAAGIAG IFMETHPDPD 250 260 270 280 RALSDGPNSW PLAKMQPLLE TLKELDKVVK NAGFLEQSSE

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO91171.1.
RefSeq	NP_820657.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83B43.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1675.
Proteomic databases
PRIDE	Q83B43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO91171; AAO91171; CBU_1675.
GeneID	1209586.
KEGG	cbu:CBU_1675.
PATRIC	17932091. VBICoxBur82552_1664.
Phylogenomic databases
eggNOG	COG2877.
HOGENOM	HOG000023021.
KO	K01627.
OMA	CAKLNIP.
ProtClustDB	PRK05198.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1646-MONOMER.
UniPathway	UPA00030. UPA00357; UER00474.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00056. KDO8P_synth.
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
PANTHER	PTHR21057:SF2. PTHR21057:SF2. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_COXBU

Accession

Primary (citable) accession number: Q83B43

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program