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Protein

CTP synthase

Gene

pyrG

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.

Miscellaneous

CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.

Enzyme regulationi

Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. CTP synthase (pyrG)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13Allosteric inhibitor CTP; alternate1
Binding sitei13UTP; alternate1
Metal bindingi71Magnesium1
Binding sitei71ATP1
Metal bindingi139Magnesium1
Binding sitei222Allosteric inhibitor CTP; alternate1
Binding sitei222UTP; alternate1
Binding sitei351L-glutamine; via carbonyl oxygen1
Active sitei378Nucleophile; for glutamine hydrolysis1
Binding sitei402L-glutamine1
Binding sitei469L-glutamine; via amide nitrogen1
Active sitei5141
Active sitei5161

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 19ATP6
Nucleotide bindingi146 – 148Allosteric inhibitor CTP3
Nucleotide bindingi186 – 191Allosteric inhibitor CTP; alternate6
Nucleotide bindingi186 – 191UTP; alternate6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPyrimidine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP synthase (EC:6.3.4.2)
Alternative name(s):
Cytidine 5'-triphosphate synthase
Cytidine triphosphate synthetase
Short name:
CTP synthetase
Short name:
CTPS
UTP--ammonia ligase
Gene namesi
Name:pyrG
Ordered Locus Names:CBU_1682
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002661011 – 555CTP synthaseAdd BLAST555

Proteomic databases

PRIDEiQ83B36.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi227377.CBU_1682.

Structurei

3D structure databases

ProteinModelPortaliQ83B36.
SMRiQ83B36.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini290 – 541Glutamine amidotransferase type-1Add BLAST252

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 265Amidoligase domainAdd BLAST265
Regioni379 – 382L-glutamine binding4

Sequence similaritiesi

Belongs to the CTP synthase family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C8D. Bacteria.
COG0504. LUCA.
HOGENOMiHOG000077515.
KOiK01937.
OMAiEFNNAYR.

Family and domain databases

CDDicd01746. GATase1_CTP_Synthase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR033828. GATase1_CTP_Synthase.
IPR027417. P-loop_NTPase.
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiView protein in Pfam
PF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q83B36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRYIFITGG VVSSLGKGIT SASLGAILEA QGLTVTLLKL DPYINVDPGT
60 70 80 90 100
MSPFQHGEVF VTEDGAETDL DLGHYERFVN ATMTRKNNFT TGRVYADVIR
110 120 130 140 150
KERRGDYLGG TIQVIPHITD EIKAKIREGA DGADVALVEV GGTVGDIESL
160 170 180 190 200
PFLEAIRQMR IELGDQQTLF IHLTLVPYVA VAGEIKTKPT QHSVKELRSI
210 220 230 240 250
GIQPDILVCR SEQPLPDAER AKIALFTNVP EPSVISLSDV KSIYEIPLIL
260 270 280 290 300
RDQGLGNRVC EKLNIKATAA DLDDWKKVVQ AQKNPRHTVT VAVVGKYVDL
310 320 330 340 350
EDSYKSLSEA LIHAGIHTQT RVVIEYIDSE AIELHGTELL KKVDAILVPG
360 370 380 390 400
GFGSRGIEGK ILAAQYAREN GIPYFGICLG MQIAIIEFAR DKAQMENANS
410 420 430 440 450
TEFDPKTPFP VVALVSEWMA KEGIIEKRKW GDDLGGTMRL GGQPCRLKID
460 470 480 490 500
SLARRLYGED RVIERHRHRY EVNNDLIGEL EKKGLVISGR SIDDRLVEMI
510 520 530 540 550
ELADHPWFVG CQFHPEFTST PRKGHPLFIG FIKAGLAAKE AKKAVLAAPS

QEKTD
Length:555
Mass (Da):61,224
Last modified:June 1, 2003 - v1
Checksum:i2BA610F28796742C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA. Translation: AAO91178.1.
RefSeqiNP_820664.1. NC_002971.3.
WP_010958369.1. NC_002971.4.

Genome annotation databases

EnsemblBacteriaiAAO91178; AAO91178; CBU_1682.
GeneIDi1209593.
KEGGicbu:CBU_1682.
PATRICifig|227377.7.peg.1671.

Similar proteinsi

Entry informationi

Entry nameiPYRG_COXBU
AccessioniPrimary (citable) accession number: Q83B36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 1, 2003
Last modified: June 7, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Coxiella burnetii
    Coxiella burnetii (strain RSA 493): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families