Reviewed, UniProtKB/Swiss-Prot Q83B06 (GCST_COXBU)

Names and origin

Protein names

Recommended name:
    Aminomethyltransferase
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	CBU_1716

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity. HAMAP MF_00259
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Aminomethyltransferase HAMAP MF_00259		PRO_0000122553

Sequences

	Sequence		Length	Mass (Da)
	Q83B06-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 794607389EB80863	FASTA	363	40,294
	10 20 30 40 50 60 MARHSPLYEE HAKAKAHFVD FAGWEMPLHY GSQIEEHHQV RQRAGIFDVS HMGVIDLEGE 70 80 90 100 110 120 EATAFLRYLL ANDVAKLSDV GRALYTCMLN PQGGVIDDLI VYRVAPTGYR LVVNAATRDK 130 140 150 160 170 180 DIAWIKEKGA GYKVSISERP EMCILAVQGP QAIAAAKSIF DEALYAQLEA LKPFHFISSP 190 200 210 220 230 240 TRDLQIARTG YTGEDGLEII VPASRATDLW ARFVHQGVKP CGLGARDTLR LEAGLNLYGT 250 260 270 280 290 300 DMDETTSPLI SNLSWTVSWN DADRNFIGRR ALEKQLDENV KERLIGLVME EPGILRNHQK 310 320 330 340 350 360 VWLTEDGEGI ITSGGFSPTL GHAIALARVP VGEVEKATVE RRGKKIPVKI IKPPFVRRGK KTF

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO91211.2. Different initiation.
RefSeq	NP_820697.2.
3D structure databases
SMR	Q83B06. Positions 4-362.
ModBase	Search...
Genome annotation databases
GeneID	1209627.
GenomeReviews	Gene locus CBU_1716 in contig AE016828_GR.
KEGG	cbu:CBU_1716.
NMPDR	fig|227377.1.peg.1641.
TIGR	CBU_1716.
Phylogenomic databases
HOGENOM	HBG299834.
OMA	KALYGGM.
PhylomeDB	Q83B06.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_1716-MONOMER.
BRENDA	2.1.2.10. 256353.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. [Graphical view]
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_COXBU

Accession

Primary (citable) accession number: Q83B06

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)