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Protein

Erythronate-4-phosphate dehydrogenase

Gene

pdxB

Organism
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the oxidation of erythronate-4-phosphate to 3-hydroxy-2-oxo-4-phosphonooxybutanoate.UniRule annotation

Catalytic activityi

4-phospho-D-erythronate + NAD+ = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + NADH.UniRule annotation

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Erythronate-4-phosphate dehydrogenase (pdxB)
  3. Phosphoserine aminotransferase (serC)
  4. 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA)
  5. Pyridoxine 5'-phosphate synthase (pdxJ)
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei46SubstrateUniRule annotation1
Binding sitei67SubstrateUniRule annotation1
Binding sitei147NADUniRule annotation1
Binding sitei175NAD; via carbonyl oxygenUniRule annotation1
Active sitei208UniRule annotation1
Binding sitei228NADUniRule annotation1
Active sitei233UniRule annotation1
Active sitei250Proton donorUniRule annotation1
Binding sitei253NAD; via amide nitrogenUniRule annotation1
Binding sitei254SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • pyridoxine biosynthetic process Source: TIGR

Keywordsi

Molecular functionOxidoreductase
Biological processPyridoxine biosynthesis
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00244; UER00310

Names & Taxonomyi

Protein namesi
Recommended name:
Erythronate-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.290UniRule annotation)
Gene namesi
Name:pdxBUniRule annotation
Ordered Locus Names:CBU_1812
OrganismiCoxiella burnetii (strain RSA 493 / Nine Mile phase I)
Taxonomic identifieri227377 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesCoxiellaceaeCoxiella
Proteomesi
  • UP000002671 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000759741 – 366Erythronate-4-phosphate dehydrogenaseAdd BLAST366

Proteomic databases

PRIDEiQ83AR8

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi227377.CBU_1812

Structurei

3D structure databases

ProteinModelPortaliQ83AR8
SMRiQ83AR8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CJ0 Bacteria
COG0111 LUCA
HOGENOMiHOG000234432
KOiK03473
OMAiSAPGCNA

Family and domain databases

CDDicd12158 ErythrP_dh, 1 hit
Gene3Di3.30.1370.170, 1 hit
HAMAPiMF_01825 PdxB, 1 hit
InterProiView protein in InterPro
IPR006139 D-isomer_2_OHA_DH_cat_dom
IPR029753 D-isomer_DH_CS
IPR006140 D-isomer_DH_NAD-bd
IPR020921 Erythronate-4-P_DHase
IPR024531 Erythronate-4-P_DHase_dimer
IPR036291 NAD(P)-bd_dom_sf
IPR038251 PdxB_dimer_sf
PANTHERiPTHR42938:SF3 PTHR42938:SF3, 1 hit
PfamiView protein in Pfam
PF00389 2-Hacid_dh, 1 hit
PF02826 2-Hacid_dh_C, 1 hit
PF11890 DUF3410, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00671 D_2_HYDROXYACID_DH_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q83AR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKILADDRI PFVSELFGDF GELILKPGAH IQNRDLLAVN ALLTRSITSV
60 70 80 90 100
DSALLEGTAV EFVGSATAGF DHIDSTWLKK QSIHWAYAPG ANATAVAEYV
110 120 130 140 150
LHCVAYLHKK NLLPRKSATA AIIGVGHVGC VVSDRLRKIG FTVFHNDPPR
160 170 180 190 200
AQLEKDFISV PLASLANVDL VCLHTPLVKT GNFPTYHLID NRFLKMLKPG
210 220 230 240 250
SVLLNAGRGA VIDNNALLQC DHVITCLDVW ENEPTVNLQL LEKTTIATPH
260 270 280 290 300
IAGYSKQAKL RATLMIYDAF LKYFHLSDTR RFSELQQLQE TMTLNIQDGR
310 320 330 340 350
NAEDILLTLF DPGRESQRMR ETLAENPDQF EYLRRHFPLR NEFSAIQLTP
360
TPSALLRKEL DDWGFK
Length:366
Mass (Da):40,969
Last modified:April 13, 2004 - v2
Checksum:iFC5F34670DCF8C1F
GO

Sequence cautioni

The sequence AAO91305 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016828 Genomic DNA Translation: AAO91305.1 Different initiation.
RefSeqiNP_820791.1, NC_002971.3

Genome annotation databases

EnsemblBacteriaiAAO91305; AAO91305; CBU_1812
GeneIDi1209723
KEGGicbu:CBU_1812
PATRICifig|227377.7.peg.1798

Similar proteinsi

Entry informationi

Entry nameiPDXB_COXBU
AccessioniPrimary (citable) accession number: Q83AR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: March 28, 2018
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health