Reviewed, UniProtKB/Swiss-Prot Q83AQ1 (KPRS_COXBU)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:	prs
Synonyms:	prsA
Ordered Locus Names:	CBU_1830

Organism

Coxiella burnetii [Complete proteome] [HAMAP]

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Caution	Tyr-143 is present instead of the conserved Asp which is expected to be an magnesium-binding residue.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141132
Regions
	Region	217 – 230	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	132	1	Magnesium Potential
	Metal binding	134	1	Magnesium Potential
	Metal binding	147	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q83AQ1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 50018B873C8597CE	FASTA	319	35,221
	10 20 30 40 50 60 MPNVDDIRIF HGSANPSLAE NVAKELNTTI GNALISRFSD GEIRFEIEEN VRGRDIYLIQ 70 80 90 100 110 120 STGHPTNEHV MELILMGDAF RRASAASITA VVPYFGYARQ DRRVRSSRVP ISAKVVADMM 130 140 150 160 170 180 QKVGFSRLIT VDLHADQIQG FFYMPVDNIY ASITALEEYR LLDKLETPMI VSPDVGGVVR 190 200 210 220 230 240 ARAIAKRLND SDLAIIDKRR PAPNQAEVMN VIGNVQNRHC VIVDDIVDTA GTLCHAASAL 250 260 270 280 290 300 KEKGALTVSS YCTHPVLSGN AVKNIMDSDI DELIVTDTIP LHEEAAKCRK ITQISLSRLI 310 AETISRINQK ESVSSMFLD

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016828 Genomic DNA. Translation: AAO91323.1.
RefSeq	NP_820809.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1209742.
GenomeReviews	Gene locus CBU_1830 in contig AE016828_GR.
KEGG	cbu:CBU_1830.
NMPDR	fig|227377.1.peg.1753.
TIGR	CBU_1830.
Phylogenomic databases
HOGENOM	Q83AQ1.
OMA	NVAEIMN.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_1830-MON.
BRENDA	2.7.6.1. 256353.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_COXBU

Accession

Primary (citable) accession number: Q83AQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)