Q83AK6 (DEF2_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide deformylase 2
Short name=PDF 2
EC=3.5.1.88

Alternative name(s):
Polypeptide deformylase 2

Gene names

Name:	def2
Ordered Locus Names:	CBU_1879

Organism

Coxiella burnetii

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity. HAMAP MF_00163
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro peptide deformylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Peptide deformylase 2 HAMAP MF_00163		PRO_0000082777
Sites
	Active site	150	1	By similarity
	Metal binding	101	1	Iron By similarity
	Metal binding	149	1	Iron By similarity
	Metal binding	153	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q83AK6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 079A74F285750B25	FASTA	209	23,979
	10 20 30 40 50 60 MKIVTTDSEN KAVLYKVASE VEIPLTKTTK EKIEAMRIFY KSFQGKAGFA VPQVGLSERI 70 80 90 100 110 120 ILVEQHLFDT TMAEETDEPT ILVNPSWRPI SDKKEWDIEG CLSVPGKVGV VERYVHVELT 130 140 150 160 170 180 AWLYHSDTEA LSKIKREYHR EYSSVLWQHE IDHLEGKIYV DKAKLLLNEK DFYSFRQQLI 190 200 ESGKIQSGMA LFDLGPLIYD IVVKGEIPS

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO91370.1.
RefSeq	NP_820856.1. NC_002971.3.
3D structure databases
ProteinModelPortal	Q83AK6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1209792.
GenomeReviews	Gene locus CBU_1879 in contig AE016828_GR.
KEGG	cbu:CBU_1879.
NMPDR	fig|227377.1.peg.1800.
PATRIC	17932489. VBICoxBur82552_1861.
TIGR	CBU_1879.
Phylogenomic databases
HOGENOM	HBG432800.
OMA	REYHREY.
ProtClustDB	CLSK915093.
Enzyme and pathway databases
BioCyc	CBUR227377:CBU_1879-MONOMER.
Family and domain databases
HAMAP	MF_00163. Pep_deformylase. [Tree]
InterPro	IPR000181. Fmet_deformylase. IPR023635. Peptide_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KO	K01462.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
SUPFAM	SSF56420. Fmet_deformylase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF2_COXBU

Accession

Primary (citable) accession number: Q83AK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program